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Offlinearraguy
Registered: 05/13/11
Posts: 451
Last seen: 10 years, 1 month
Using H202 for making spore syringes
    #17329114 - 12/03/12 08:27 PM (12 years, 1 month ago)

I wanted to make this post because A LOT of people told me that this was stupid to do and it wouldnt work.

I watched this video
and i decided I would try it out.

So i got a spore print, printed it on tin foil. (the foil was in the pc with some of my jars, so it was pretty clean. I foiled the tin foil up and left it in a bag for a week or two. When I inoculating jars I decided to make that print into a spore syringe. I got some H202 3% i am pretty sure, just the normal stuff you use for cuts. (i sterilized my syringe as well in my pc) I got some H202 in my syringe, squirted it at the spore print which was in the jar, I did this a couple of times so the spores would get off the foil and be in the H202. I sucked it all up and inoculated a WBS jar. Maybe a week and a half later (not 100% sure about the time frame) I am seeing quite a bit of myc. NO contamination at all!

Note: this was my first time doing this, I will defiantly try to do this more and more times with the prints i collect and tell you how it goes. Also this was a little slower compared to my spore syringes I bought from a vendor because I read that H202 stuns the spore (not 100% about that either) But it turned out great!


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Offlinejjjcmzzt
That guy
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Registered: 06/17/12
Posts: 624
Loc: United States
Last seen: 3 years, 10 months
Re: Using H202 for making spore syringes [Re: arraguy]
    #17329163 - 12/03/12 08:35 PM (12 years, 1 month ago)

Sounds awesome! Im always having contam problems so when this grow finishes and i get a bunch of spore prints im definitely gonna try this, i have a lot of H2O2 so im set!


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Psychedelics i want to take: Mushrooms, Salvia (weak), Salvia (strong), Cannabis, LSA, Cactus, LSD, DMT, Bufotenine, 5-meo-DMT, 4-aco-DMT, Bufocin (theoretical as of now),  and a long long time from now, Datura (deliriant).

"LSD is a psychedelic substance which occasionally causes psychotic behavior in people that have never taken it."

DEATH METAL \m/

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Offlinearraguy
Registered: 05/13/11
Posts: 451
Last seen: 10 years, 1 month
Re: Using H202 for making spore syringes [Re: jjjcmzzt]
    #17329190 - 12/03/12 08:38 PM (12 years, 1 month ago)

Quote:

jjjcmzzt said:
Sounds awesome! Im always having contam problems so when this grow finishes and i get a bunch of spore prints im definitely gonna try this, i have a lot of H2O2 so im set!



Defiantly PM me and tell me how everything works, I am NOT saying this is going to work 100% of the time or anything like that. I have just tried this once so far and it turned out good, I was SHOCKED! Everyone I talked to said it would fail, said that I wouldn't see any colonization.


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InvisibleFooMan
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Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
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Re: Using H202 for making spore syringes [Re: jjjcmzzt]
    #17329227 - 12/03/12 08:43 PM (12 years, 1 month ago)

Peroxide can kill spores and stunt mycelium. Why would you want to use it on your prints? Try to make clean prints to get clean syringes. If it worked, more power to you, but I'd wait until you actually see fruits before deciding this was a total success.


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Offlinejjjcmzzt
That guy
Male
Registered: 06/17/12
Posts: 624
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Last seen: 3 years, 10 months
Re: Using H202 for making spore syringes [Re: arraguy]
    #17329243 - 12/03/12 08:46 PM (12 years, 1 month ago)

Quote:

arraguy said:
Quote:

jjjcmzzt said:
Sounds awesome! Im always having contam problems so when this grow finishes and i get a bunch of spore prints im definitely gonna try this, i have a lot of H2O2 so im set!



Defiantly PM me and tell me how everything works, I am NOT saying this is going to work 100% of the time or anything like that. I have just tried this once so far and it turned out good, I was SHOCKED! Everyone I talked to said it would fail, said that I wouldn't see any colonization.




Yea i think that it wouldnt work for long term storage, you would have to use the syringe ASAP, to make sure most of the spores are still viable. Either way, my grow bags are almost colonized, then its onto the fruiting chamber and THEN i can try this. Im only gonna use a couple jars to start though, and go from there.


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Psychedelics i want to take: Mushrooms, Salvia (weak), Salvia (strong), Cannabis, LSA, Cactus, LSD, DMT, Bufotenine, 5-meo-DMT, 4-aco-DMT, Bufocin (theoretical as of now),  and a long long time from now, Datura (deliriant).

"LSD is a psychedelic substance which occasionally causes psychotic behavior in people that have never taken it."

DEATH METAL \m/

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Offlinearraguy
Registered: 05/13/11
Posts: 451
Last seen: 10 years, 1 month
Re: Using H202 for making spore syringes [Re: FooMan]
    #17329275 - 12/03/12 08:49 PM (12 years, 1 month ago)

Quote:

FooMan said:
Peroxide can kill spores and stunt mycelium. Why would you want to use it on your prints? Try to make clean prints to get clean syringes. If it worked, more power to you, but I'd wait until you actually see fruits before deciding this was a total success.



Yup thats what everyone told me, That H202 KILLS spores, but I was thinking because its only about 3% or so, it didnt effect the spore too much. I will defiantly wait until fully colonize and try this again and again. I will keep this forum updated!


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InvisibleFooMan
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Re: Using H202 for making spore syringes [Re: arraguy]
    #17329353 - 12/03/12 09:01 PM (12 years, 1 month ago)

3% is the normal strength. Peroxide weakens over time too. previously opened bottles that have been sitting around won't be as effective as fresh, unopened bottles. Again, if this worked for you, great. But I sure as hell wouldn't recommend it as a reliable method for making spore syringes, just like the oven tek was a bad idea for creating a sterile work environment even though some people swore by it. I'm all for experimenting, but peroxide definitely kills spores & germs. The fact some of your spores may have survived doesn't make this a good idea.


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Offlinearraguy
Registered: 05/13/11
Posts: 451
Last seen: 10 years, 1 month
Re: Using H202 for making spore syringes [Re: FooMan]
    #17329430 - 12/03/12 09:13 PM (12 years, 1 month ago)

Quote:

FooMan said:
3% is the normal strength. Peroxide weakens over time too. previously opened bottles that have been sitting around won't be as effective as fresh, unopened bottles. Again, if this worked for you, great. But I sure as hell wouldn't recommend it as a reliable method for making spore syringes, just like the oven tek was a bad idea for creating a sterile work environment even though some people swore by it. I'm all for experimenting, but peroxide definitely kills spores & germs. The fact some of your spores may have survived doesn't make this a good idea.



Please read everything I said again if you think i said this was a good idea... I never said such a thing. But my Peroxide bottle was newly bought from the store and unopened.
All i said was that this worked perfectly my first time. I will be trying this over and over again and I will note the success rate. Maybe and maybe then I will swear or not swear by this. Who knows... I havnt seen anyone else giving this a shot... I have read other people asking about this and I was just noting for people what happened for me, THATS ALL!


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Edited by arraguy (12/03/12 09:14 PM)

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