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FeelFree


Registered: 06/27/12
Posts: 193
Last seen: 9 months, 12 days
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Last minute tips?
#17315054 - 12/01/12 02:31 PM (12 years, 1 month ago) |
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I will be growing shiitake mushrooms for my first grow.
Today I'm be blending down some organic brown rice into a flour. I will also be using this vermiculite.
I will boil my jars in a large pot with a lid for 90 minutes, because I do not own a pressure cooker. I will let them cool overnight, just to be safe.
These are the jars I am using  Kerr regular mouth mason jars.
The spores are on a print made by one of the sponsors. I'm going to scrape the spores into sterile water using a fresh sterilized blade. (I know that scraping the spores into the water doesn't have to the that sterile but what can it hurt )
Then with a pre-sterilized packaged syringe, I will draw up the suspension. I will heat up the tip of the needle, then put the cap back on.
With my spore syringe I will inject about a cc for each of the 4 inoculation holes (wiping with alcohol in between. Then I will put them in a safe place and wait.
Any advice is welcome.
Thanks.
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Destitute
floss daily

Registered: 11/28/12
Posts: 4,027
Loc: where
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Re: Last minute tips? [Re: FeelFree]
#17315243 - 12/01/12 03:04 PM (12 years, 1 month ago) |
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Sounds good but only need 1/4 cc per hole
-------------------- [quote]Prisoner#1 said:
I've done your mother's crack[/quote]
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: Last minute tips? [Re: Destitute]
#17315355 - 12/01/12 03:21 PM (12 years, 1 month ago) |
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It's recommended not to use a blade to scrape sores as it can damage them...instead make an inoculation loop out of a small paper clip.
You can stretch the hell out of those spores as they are very potent, only a few drops per hole will be fine, just remember to shake the syringe before each jar.
EDIT: Also, a descent print can make 20-30 spore syringes.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (12/01/12 03:23 PM)
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Last minute tips? [Re: Destitute]
#17315395 - 12/01/12 03:26 PM (12 years, 1 month ago) |
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Scraping the spores into the water has to be very sterile or else you will bring along many different forms of contamination for the ride.
You have not mentioned using a glove box while making your syringe, this is a necessity.
If you have any other questions fire away, also taking a look at this write up may answer some questions or clarify some procedures: https://www.shroomery.org/forums/showflat.php/Number/15223324/fpart/1
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FeelFree


Registered: 06/27/12
Posts: 193
Last seen: 9 months, 12 days
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The sawdust replacement for vermiculite seems like a good idea for my shiitake grow.
Edited by FeelFree (12/01/12 10:10 PM)
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Last minute tips? [Re: FeelFree]
#17317810 - 12/01/12 10:26 PM (12 years, 1 month ago) |
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In my experience you will benefit more from adding sawdust to your verm and brf as opposed to substituting the verm for sawdust.
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FeelFree


Registered: 06/27/12
Posts: 193
Last seen: 9 months, 12 days
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Do I need a PC for sawdust?
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Last minute tips? [Re: FeelFree]
#17320202 - 12/02/12 11:44 AM (12 years, 1 month ago) |
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Steam sterilization should be sufficient.
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