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OfflineRogerRabbitM
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Re: Agar Issues [Re: Amanita virosa]
    #17313622 - 12/01/12 08:10 AM (12 years, 1 month ago)

Isolate more if you want single sectors.  You might even start over from scratch, using minimum spores this time.  When you have many thousands of strains on the same dish, it can take a lot of transfers.

Quote:

Amanita virosa said:
The first plate looks like two incompatible strains to me. If they were compatible they would have formed dikaryotic hyphae. But it is really hard to see in that shot. Is there a clear line without Myc where they come together?  In other words are they repelling each other?  If so they are incompatible strains. You can always just put a small wedge from say five different plates in each jar to increase likelihood of compatibility




Just to clarify something, strains already are dikaryotic.  Hyphae are monokaryotic.  If compatible mononucleate hyphae(monokaryotic mycelium) meet, they exchange DNA and become polynucleate (dikaryotic).  You can't have dikaryotic hyphae as that would be like a two wheeled monocycle.
RR


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OfflineFuckUp Eddy
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Re: Agar Issues [Re: RogerRabbit]
    #17313836 - 12/01/12 09:22 AM (12 years, 1 month ago)

So what do these seams mean??? I need to start over like you said. Cloning would probably eliminate this many transfers Im thinking?


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If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

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OfflineAmanita virosa
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Re: Agar Issues [Re: FuckUp Eddy]
    #17314856 - 12/01/12 01:39 PM (12 years, 1 month ago)

Quote:

RogerRabbit said:
Isolate more if you want single sectors.  You might even start over from scratch, using minimum spores this time.  When you have many thousands of strains on the same dish, it can take a lot of transfers.

Quote:

Amanita virosa said:
The first plate looks like two incompatible strains to me. If they were compatible they would have formed dikaryotic hyphae. But it is really hard to see in that shot. Is there a clear line without Myc where they come together?  In other words are they repelling each other?  If so they are incompatible strains. You can always just put a small wedge from say five different plates in each jar to increase likelihood of compatibility




Just to clarify something, strains already are dikaryotic.  Hyphae are monokaryotic.  If compatible mononucleate hyphae(monokaryotic mycelium) meet, they exchange DNA and become polynucleate (dikaryotic).  You can't have dikaryotic hyphae as that would be like a two wheeled monocycle.
RR



When i first looked at the pict, I was thinking that he may have had two monokaryotic hyphae, which each came from a single spore, on one plate and that they may have not been appropriate mating strains. I guess this is unlikely if you shoot multispore into agar then sub it out cause it only takes two appropriate spores to form a dikaryon and he would have likely shot 1000s onto the plate. I was basing it on some work I did with rhizopus where you start two plus strains (or two minus strains) on opposite sides of an agar plate and where they meet, they will never touch always maintaining a 2 mm clear zone. I.e.like strains repel each other. I realize that the mating system in basidiomycetes is more complex than plus and minus (zygomycota) but could the same thing not happen?

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OfflineFuckUp Eddy
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Re: Agar Issues [Re: Amanita virosa]
    #17315088 - 12/01/12 02:38 PM (12 years, 1 month ago)

I think the one on the left is mold though...compromising the whole dish. Lots of my agar to brf (to proof) has turned out to be mold. In the beginning it looked like the culture on the left. Frustrating. Only three of my seven cultures to brf turned out to be mycellium.


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If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

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OfflineFuckUp Eddy
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Registered: 10/29/12
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Re: Agar Issues [Re: FuckUp Eddy]
    #17321843 - 12/02/12 04:42 PM (12 years, 1 month ago)

Is this mold? This is from a clone, 2nd transfer...It doesn't look right to me. Just too whispy. Why are parts of the outside of the circle like that?



--------------------
If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

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Offline00funG.I.00
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Registered: 11/03/12
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Re: Agar Issues [Re: FuckUp Eddy]
    #17322968 - 12/02/12 08:04 PM (12 years, 1 month ago)

looks like mycellium that has been influenced by fluctuating temps to me...or maybe directional to the heatsource... look under a scope to be certain...

Edited by 00funG.I.00 (12/02/12 08:07 PM)

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OfflineFuckUp Eddy
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Registered: 10/29/12
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Re: Agar Issues [Re: 00funG.I.00]
    #17323023 - 12/02/12 08:12 PM (12 years, 1 month ago)

Dammit I don't have one. You are exactly right though with the fluctuating temps.

It's really hard to make transfers from something like this. Where the hell would I?


--------------------
If you havent played with this then you are missin out! http://www.albinoblacksheep.com/flash/zoomquilt

Also good video!!! http://www.youtube.com/watch?v=6O6x_m4zvFs

Extras: Filter Print Post Top
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