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MoGrow
Lysergic Funeral Procession


Registered: 11/16/12
Posts: 1,356
Loc: Da Sunshine State
Last seen: 2 years, 2 months
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Bulk Supplies...
#17313275 - 12/01/12 06:10 AM (12 years, 1 month ago) |
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I just ordered most everything i need for my adventures in bulk, can i get some feedback on these items to assure there the right ones before my order is past the cancellation time. Please and thank you shroomerites.
In addition to this i also ordered 20x 100mm Petri Dishes for isolation.
Thanks in advance for your taking the time to double check my supply list for me.
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Oeric McKenna said:
"My innoculant is heavily contaminated with mushroom mycelium"
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
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Re: Bulk Supplies... [Re: MoGrow]
#17313285 - 12/01/12 06:14 AM (12 years, 1 month ago) |
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your gonna need alot more petris than that, and i would invest in some sfds sometime in the future also
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MoGrow
Lysergic Funeral Procession


Registered: 11/16/12
Posts: 1,356
Loc: Da Sunshine State
Last seen: 2 years, 2 months
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Re: Bulk Supplies... [Re: twistedty]
#17313297 - 12/01/12 06:17 AM (12 years, 1 month ago) |
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How many Petris would you suggest.. keep in mind this is my first attempt at isolation / bulk grows so im sure there will be marginal errors on my part.
-------------------- Trades
Current Contest!
My current mono (pics)
Ghetto Bulk Dehydrator Tek
How I Made My Alcohol Lamp
Oeric McKenna said:
"My innoculant is heavily contaminated with mushroom mycelium"
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
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Re: Bulk Supplies... [Re: MoGrow]
#17313307 - 12/01/12 06:21 AM (12 years, 1 month ago) |
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Trippy_Smurf
Sketchy Mother Fucker




Registered: 02/14/11
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Loc: Smurf Villiage
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Re: Bulk Supplies... [Re: twistedty]
#17313534 - 12/01/12 07:40 AM (12 years, 1 month ago) |
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-------------------- SECURITY: READ THIS!
Chef: Kids, what did I tell you about drugs?
Kids: There's a time and a place for everything, and it's called college.
How to be a good shroomie How to grow mushrooms A collection of good links (may be outdated) How things should look How to pass a drug test
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MoGrow
Lysergic Funeral Procession


Registered: 11/16/12
Posts: 1,356
Loc: Da Sunshine State
Last seen: 2 years, 2 months
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Re: Bulk Supplies... [Re: twistedty]
#17313577 - 12/01/12 07:54 AM (12 years, 1 month ago) |
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Quote:
twistedty said: its always cheaper to buy in bulk, and if you're new its gonna take a few runs to get agar down pat.
a case of 500
http://www.ebay.com/itm/Sterile-Polystyrene-Petri-Dish-Plate-100x15-mm-500-cs-/120925999897?pt=LH_DefaultDomain_0&hash=item1c27c05319
Ordered and thx for the info.
-------------------- Trades
Current Contest!
My current mono (pics)
Ghetto Bulk Dehydrator Tek
How I Made My Alcohol Lamp
Oeric McKenna said:
"My innoculant is heavily contaminated with mushroom mycelium"
Edited by MoGrow (12/01/12 07:55 AM)
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
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Re: Bulk Supplies... [Re: MoGrow]
#17313621 - 12/01/12 08:09 AM (12 years, 1 month ago) |
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Quote:
MoGrow said:
Quote:
twistedty said: its always cheaper to buy in bulk, and if you're new its gonna take a few runs to get agar down pat.
a case of 500
http://www.ebay.com/itm/Sterile-Polystyrene-Petri-Dish-Plate-100x15-mm-500-cs-/120925999897?pt=LH_DefaultDomain_0&hash=item1c27c05319
Ordered and thx for the info.
np bro, if you get tired of mixing and making agar, you can also order PDA from mycosupply, its just add water.
agar is the way to go, once you get it you wont use another syringe again
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Loc: Seattle
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Re: Bulk Supplies... [Re: MoGrow]
#17313630 - 12/01/12 08:12 AM (12 years, 1 month ago) |
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Don't use high temp RTV as it doesn't cure well at the low temps we use and tends to peel off. If you insist on using silicone on your lids, use the regular silicone, not the red high temp stuff. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Z O M G
Stranger Danger



Registered: 06/16/12
Posts: 730
Loc: new york.
Last seen: 10 years, 1 month
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Quote:
RogerRabbit said: Don't use high temp RTV as it doesn't cure well at the low temps we use and tends to peel off. If you insist on using silicone on your lids, use the regular silicone, not the red high temp stuff. RR
well that just goes against everything i thought i knew.
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Zarotti
Stranger

Registered: 05/12/12
Posts: 314
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Quote:
RogerRabbit said: Don't use high temp RTV as it doesn't cure well at the low temps we use and tends to peel off. If you insist on using silicone on your lids, use the regular silicone, not the red high temp stuff. RR
I used normal silicone for my jars only had one run so far at 15 psi for 90 minutes and nothing melted away - 2nd run it started crumbling...
Edited by Zarotti (12/02/12 03:33 AM)
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brbphone


Registered: 10/26/12
Posts: 76
Last seen: 11 years, 9 months
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Re: Bulk Supplies... [Re: Zarotti]
#17314261 - 12/01/12 11:15 AM (12 years, 1 month ago) |
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Quote:
Zarotti said:
Why do most members recommend the use of the expensive heatresistant silicone ?
People like to over think everything.
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OmegaMachine
Stranger

Registered: 10/02/12
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Last seen: 10 years, 10 months
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Re: Bulk Supplies... [Re: Zarotti]
#17314266 - 12/01/12 11:16 AM (12 years, 1 month ago) |
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The tube might have been a dollar more for the high temp. I figured it was going into the PC so why not. Haven't had any peeling issues yet, although the jars are still pretty new.
-------------------- -Grain to Grain - Will never use another method as long as this is an option.
-Coir - Cheap/Effective/EASY
-Monotubs - So hands free Edward Scissorhands could do this shit.
-SAB - See Coir.
Earthlings
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skippydude
Myco-curious



Registered: 09/08/12
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Loc: "Alice's Wonderland"
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skippydude
Myco-curious



Registered: 09/08/12
Posts: 1,827
Loc: "Alice's Wonderland"
Last seen: 10 years, 1 month
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Spending all this money on agar supplies,do you have a flow hood?
It will save you more money in the long run in dishes than any Ebay sale!
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MoGrow
Lysergic Funeral Procession


Registered: 11/16/12
Posts: 1,356
Loc: Da Sunshine State
Last seen: 2 years, 2 months
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i guess it would have helped if i had mentioned that The space suit is to be cut up.. its my source for tyvek i was going to use this lid tek i came across here on the shroomery for grain lids.. i cant find the exact tek (I'm on my laptop at work and my shits bookmarked at home) but this one is very similar.
http://www.shroomery.org/forums/showflat.php/Number/8364158#8364158
Damion5050's coir tek is what im using for my first bulk.
Quote:
RogerRabbit said: Don't use high temp RTV as it doesn't cure well at the low temps we use and tends to peel off. If you insist on using silicone on your lids, use the regular silicone, not the red high temp stuff. RR
I made a LC lid using regular 100% silicone and its doing fine its been cooked 2x now. But I've never used a PC before and figured the lid tek was calling for HT RTV because of the PC. Thats awesome save me 9$ or w/e it is because I've a whole tube of reg at the house -1 lid. Thanks for the heads up RR 
Quote:
skippydude said: Spending all this money on agar supplies,do you have a flow hood?
It will save you more money in the long run in dishes than any Ebay sale!
No i dont have a FH i pretty much bought the agar and petries to play with in a SAB. Ill be making MS syringes from harvest prints, as well as cloning from fruit bodies. If my SAB agar project fails no biggie. It will give me plenty of practice, but i think i can be sterile enough to make it work.
-------------------- Trades
Current Contest!
My current mono (pics)
Ghetto Bulk Dehydrator Tek
How I Made My Alcohol Lamp
Oeric McKenna said:
"My innoculant is heavily contaminated with mushroom mycelium"
Edited by MoGrow (12/01/12 02:36 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: Bulk Supplies... [Re: MoGrow]
#17315079 - 12/01/12 02:35 PM (12 years, 1 month ago) |
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Quote:
MoGrow said: No i dont have a FH i pretty much bought the agar and petries to play with in a SAB. Ill be making MS syringes from harvest prints, as well as cloning from fruit bodies. If my SAB agar project fails no biggie. It will give me plenty of practice, but i think i can e sterile enough ton make it work.
A flow hood is not needed for agar work. A SAB will work just fine.
A still air box doesn't even have to be sanitary, all it has to do is provide a "still air" environment.
If the air is still, it's impossible for a contam to blow on your work.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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serotonin storm
Don't be scared, homie!


Registered: 07/15/12
Posts: 1,178
Loc: Holland
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Re: Bulk Supplies... [Re: MoGrow]
#17315097 - 12/01/12 02:39 PM (12 years, 1 month ago) |
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You'll definitely need more than 20 dishes. It can take up to 20 dishes for just one round of isolates. Considering you start from spores, you'll potentially have hundreds of sub strains on one dish, and you'll need to isolate more than just one of those because there is a chance that you isolate a non producing strain, in which case you've wasted your time. But if you isolate 4-6 sub strains from the starter dish your chances of getting a fruit producing strain is better
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Montock
Time = $


Registered: 08/27/09
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Last seen: 11 years, 9 months
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Re: Bulk Supplies... [Re: PussyFart]
#17315185 - 12/01/12 02:53 PM (12 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
MoGrow said: No i dont have a FH i pretty much bought the agar and petries to play with in a SAB. Ill be making MS syringes from harvest prints, as well as cloning from fruit bodies. If my SAB agar project fails no biggie. It will give me plenty of practice, but i think i can e sterile enough ton make it work.
A flow hood is not needed for agar work. A SAB will work just fine.
A still air box doesn't even have to be sanitary, all it has to do is provide a "still air" environment.
If the air is still, it's impossible for a contam to blow on your work.
qft , theyre actually better, the only thing you need to focus on in a sab is a contam falling down onto your work ( if your using the box properly)
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MoGrow
Lysergic Funeral Procession


Registered: 11/16/12
Posts: 1,356
Loc: Da Sunshine State
Last seen: 2 years, 2 months
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Quote:
serotonin storm said: You'll definitely need more than 20 dishes. It can take up to 20 dishes for just one round of isolates.
Quote:
MoGrow said:
Quote:
twistedty said: its always cheaper to buy in bulk, and if you're new its gonna take a few runs to get agar down pat.
a case of 500
http://www.ebay.com/itm/Sterile-Polystyrene-Petri-Dish-Plate-100x15-mm-500-cs-/120925999897?pt=LH_DefaultDomain_0&hash=item1c27c05319
Ordered and thx for the info.
Quote:
serotonin storm said: Considering you start from spores, you'll potentially have hundreds of sub strains on one dish, and you'll need to isolate more than just one of those because there is a chance that you isolate a non producing strain, in which case you've wasted your time. But if you isolate 4-6 sub strains from the starter dish your chances of getting a fruit producing strain is better
Thank u for clearing up why so many are needed i didnt know it took that many...for now tho ill be using them to clone ... as a way to get familiar with agar before i mess with isolating. I have 7 PFTEK's in a SGFC now and 10 more that will be ready to go in tuesday of this coming week. when i get fruits im gonna clone and print but im going to be doing G2G's like i say i just wanna get familiar with agar since I've never touched the stuff before.
Any info you feel like tossing my way im like a sponge. Thx for your responses, always helpful.
-------------------- Trades
Current Contest!
My current mono (pics)
Ghetto Bulk Dehydrator Tek
How I Made My Alcohol Lamp
Oeric McKenna said:
"My innoculant is heavily contaminated with mushroom mycelium"
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