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Offlinetabiyeh
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Registered: 10/01/12
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First agar attempt, no growth 5 days later *DELETED*
    #17308086 - 11/30/12 10:14 AM (12 years, 1 month ago)

Post deleted by tabiyeh

Reason for deletion: Life is lowly anonymity.


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Offlinetabiyeh
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Re: First agar attempt, no growth 5 days later *DELETED* [Re: tabiyeh]
    #17309909 - 11/30/12 03:45 PM (12 years, 1 month ago)

Post deleted by tabiyeh

Reason for deletion: Life is lowly anonymity.


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OfflinePussyFart
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
    #17310155 - 11/30/12 04:27 PM (12 years, 1 month ago)

:rules: 4.  Do not bump your own thread for at least 24 hours.  It's not fair to the other posters to have your thread constantly bumped to the top.  Violators will have their thread deleted.

You don't need a certain depth for growing a culture on agar, the mycellium will grow on a 2d plane, or flat on the surface, even if it's very thin.

If you are planning on storing the culture for some time it's better to make the agar thicker, to retain as much moisture as possible for storage.

I have no idea why its not growing...how many days has it been exactly...several days is kinda vague..

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Offlinetabiyeh
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Re: First agar attempt, no growth 5 days later *DELETED* [Re: PussyFart]
    #17310611 - 11/30/12 05:46 PM (12 years, 1 month ago)

Post deleted by tabiyeh

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OfflinePussyFart
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
    #17310726 - 11/30/12 06:01 PM (12 years, 1 month ago)

set it to 70 or 75.

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InvisibleTheEaglesGift
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
    #17310924 - 11/30/12 06:30 PM (12 years, 1 month ago)

Quote:

tabiyeh said:
Sorry, anyway it was 5 days ago. My only guess is that my room is cold but I've kept it in an incubator that's at 60 degrees.




Bump up to 70 degrees f.

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Invisibleserotonin storm
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Registered: 07/15/12
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Re: First agar attempt, no growth 5 days later [Re: TheEaglesGift]
    #17310947 - 11/30/12 06:34 PM (12 years, 1 month ago)

5 days without germination should by no means be a cause for concern. Spores can take up to or possibly longer than 3 weeks to germinate


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Offlineskippydude
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Re: First agar attempt, no growth 5 days later [Re: TheEaglesGift]
    #17310961 - 11/30/12 06:37 PM (12 years, 1 month ago)

Did a shitake LC once and a Shitake clone another time.

They took a solid 15 days to show anything.

If there isn't anything growing, means there ain't no contams yet either:rockon:

Spores take longer than LC on agar as well.

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Offlinetabiyeh
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Re: First agar attempt, no growth 5 days later *DELETED* [Re: skippydude]
    #17339308 - 12/05/12 04:02 PM (12 years, 1 month ago)

Post deleted by tabiyeh

Reason for deletion: Life is lowly anonymity.


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OfflineSpecops2594
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Registered: 10/02/12
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
    #17339476 - 12/05/12 04:30 PM (12 years, 1 month ago)

The rate of mycelial expansion is exponentially proportional to temperature to a point. 60 is VERY cold for cubensis. If 70-75 is optimal temperature, I can't imagine how long 60 would take.


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Offlineskippydude
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Re: First agar attempt, no growth 5 days later [Re: Specops2594]
    #17339540 - 12/05/12 04:44 PM (12 years, 1 month ago)

:congrats:

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