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tabiyeh



Registered: 10/01/12
Posts: 62
Last seen: 11 years, 3 months
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First agar attempt, no growth 5 days later *DELETED*
#17308086 - 11/30/12 10:14 AM (12 years, 1 month ago) |
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Post deleted by tabiyehReason for deletion: Life is lowly anonymity.
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tabiyeh



Registered: 10/01/12
Posts: 62
Last seen: 11 years, 3 months
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Re: First agar attempt, no growth 5 days later *DELETED* [Re: tabiyeh]
#17309909 - 11/30/12 03:45 PM (12 years, 1 month ago) |
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Post deleted by tabiyehReason for deletion: Life is lowly anonymity.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
#17310155 - 11/30/12 04:27 PM (12 years, 1 month ago) |
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4. Do not bump your own thread for at least 24 hours. It's not fair to the other posters to have your thread constantly bumped to the top. Violators will have their thread deleted.
You don't need a certain depth for growing a culture on agar, the mycellium will grow on a 2d plane, or flat on the surface, even if it's very thin.
If you are planning on storing the culture for some time it's better to make the agar thicker, to retain as much moisture as possible for storage.
I have no idea why its not growing...how many days has it been exactly...several days is kinda vague..
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
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tabiyeh



Registered: 10/01/12
Posts: 62
Last seen: 11 years, 3 months
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Re: First agar attempt, no growth 5 days later *DELETED* [Re: PussyFart]
#17310611 - 11/30/12 05:46 PM (12 years, 1 month ago) |
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Post deleted by tabiyehReason for deletion: Life is lowly anonymity.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
#17310726 - 11/30/12 06:01 PM (12 years, 1 month ago) |
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set it to 70 or 75.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
#17310924 - 11/30/12 06:30 PM (12 years, 1 month ago) |
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Quote:
tabiyeh said: Sorry, anyway it was 5 days ago. My only guess is that my room is cold but I've kept it in an incubator that's at 60 degrees.
Bump up to 70 degrees f.
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serotonin storm
Don't be scared, homie!


Registered: 07/15/12
Posts: 1,178
Loc: Holland
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Re: First agar attempt, no growth 5 days later [Re: TheEaglesGift]
#17310947 - 11/30/12 06:34 PM (12 years, 1 month ago) |
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5 days without germination should by no means be a cause for concern. Spores can take up to or possibly longer than 3 weeks to germinate
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skippydude
Myco-curious



Registered: 09/08/12
Posts: 1,827
Loc: "Alice's Wonderland"
Last seen: 10 years, 1 month
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Re: First agar attempt, no growth 5 days later [Re: TheEaglesGift]
#17310961 - 11/30/12 06:37 PM (12 years, 1 month ago) |
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Did a shitake LC once and a Shitake clone another time.
They took a solid 15 days to show anything.
If there isn't anything growing, means there ain't no contams yet either
Spores take longer than LC on agar as well.
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tabiyeh



Registered: 10/01/12
Posts: 62
Last seen: 11 years, 3 months
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Re: First agar attempt, no growth 5 days later *DELETED* [Re: skippydude]
#17339308 - 12/05/12 04:02 PM (12 years, 1 month ago) |
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Post deleted by tabiyehReason for deletion: Life is lowly anonymity.
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Specops2594
Shroomer



Registered: 10/02/12
Posts: 458
Loc: PNW, Oregon
Last seen: 4 years, 7 months
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Re: First agar attempt, no growth 5 days later [Re: tabiyeh]
#17339476 - 12/05/12 04:30 PM (12 years, 1 month ago) |
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The rate of mycelial expansion is exponentially proportional to temperature to a point. 60 is VERY cold for cubensis. If 70-75 is optimal temperature, I can't imagine how long 60 would take.
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skippydude
Myco-curious



Registered: 09/08/12
Posts: 1,827
Loc: "Alice's Wonderland"
Last seen: 10 years, 1 month
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Re: First agar attempt, no growth 5 days later [Re: Specops2594]
#17339540 - 12/05/12 04:44 PM (12 years, 1 month ago) |
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