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THCybin
The Journey

Registered: 01/30/12
Posts: 129
Last seen: 11 years, 6 months
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Help me
#17307820 - 11/30/12 08:48 AM (12 years, 1 month ago) |
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Hello all those out in shroomery land, I need help identifying this. What a way to start off my first post but I've been watching and trying to learn for a while now, only to have this happen to me .
This is day 4

I'm guessing from reading that it's Aspergillus flavus but I'm not sure. I prepared my substrate with the brf / verm mix (some of it fell on the floor and I picked most of it up to put back in jar, pretty sure that isn't a cause but just trying to tell everything I did for an answer). I boiled my jars on the stove for 90 minutes for sterility, waited over night. I don't have a glove box so I sprayed my bathroom down with a bleach vinegar mix that I read about on here, waited for a while then went in to do inoculation. Now I forgot to flame sterilize between each jar after the second jar I believe. After all that was done I think this next part is what caused the browning on the substrate and the yellow contams, I sat the jars inside of a box and put them on top of my computer, I believe that it was way too hot. They were there for 3 days before I removed them because they started to form the extremely brown spots where I felt the mycellium had been trying to form but now I think I'm too late. Any advice?
It's been about 30 minutes since I made this post and the longer I wait the more I just want to dump it. I was wondering since the spot isn't too big is it ok to just dump the substrate outside, and bring it back in to sterilize? I don't have a PC but I was wondering since you can sterilize substrate with a regular stove pot for the brf cakes can you do the same for a jar that only had a small amount of contaminants in it? I really don't want to throw away this jar and the same yellow spots are in about 2 other jars.
Edited by THCybin (11/30/12 09:43 AM)
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THCybin
The Journey

Registered: 01/30/12
Posts: 129
Last seen: 11 years, 6 months
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Ok never mind the id it's obviously not mycelium and I don't want to take the chance of letting it go any longer. I just need to know since it's still early do I have to still throw away the whole jar or can I just dump it outside and try to sterilize it for use again? Once again I don't have a PC, so my only option is to steam sterilize on the stove, what should I do?
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Re: Help me [Re: THCybin] 1
#17309232 - 11/30/12 01:47 PM (12 years, 1 month ago) |
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You can just dump it outside although steaming the jars first would remove any chance of contaminating yourself with mold spores in the process.
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THCybin
The Journey

Registered: 01/30/12
Posts: 129
Last seen: 11 years, 6 months
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Re: Help me [Re: Kizzle]
#17309280 - 11/30/12 01:56 PM (12 years, 1 month ago) |
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Okay thanks for that, I also was wondering is it okay to reuse the jars after dumping the substrate if I steam them first? I already got rid of the first jar completely, dumped it outside and it's gone, although I didn't steam it first, I still have three more jars with the same yellow spots popping up. The development is even smaller than in the picture shown that's why I was wondering if reusing the jars would be a problem or not.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Re: Help me [Re: THCybin] 1
#17309521 - 11/30/12 02:36 PM (12 years, 1 month ago) |
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You can reuse the jars. Soap and water will remove any mold spores along with any remaining substrate.
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THCybin
The Journey

Registered: 01/30/12
Posts: 129
Last seen: 11 years, 6 months
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Re: Help me [Re: Kizzle]
#17309637 - 11/30/12 02:56 PM (12 years, 1 month ago) |
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Okay thank you very much for the help! I guess I disposed of one jar for no reason but better safe than sorry. I'll just steam, dump, clean and save them for later, once again thank you very much.
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zMan
zMan

Registered: 09/08/12
Posts: 2,191
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Re: Help me [Re: THCybin] 1
#17310059 - 11/30/12 04:11 PM (12 years, 1 month ago) |
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For the price of a clear storage box you can have a Still Air Box (SAB) and you will more then likely cut out the contams. The bathroom is dirty and no matter what you do it there when you open the door to walk in you are still inviting ALL the contams that were outside before.
-------------------- Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Help me [Re: zMan] 1
#17311018 - 11/30/12 06:45 PM (12 years, 1 month ago) |
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Don't inoculate in kitchens or bathrooms. They're both very dirty by default. Look in your sink drain of your bathroom, pull up the stopper. Do you see black mold? The tank in the back of your toilet that holds water, see any mold?
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zMan
zMan

Registered: 09/08/12
Posts: 2,191
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Quote:
TheEaglesGift said: Don't inoculate in kitchens or bathrooms. They're both very dirty by default. Look in your sink drain of your bathroom, pull up the stopper. Do you see black mold? The tank in the back of your toilet that holds water, see any mold?
I do it in the kitchen but I spray LOOOTS of lysol.
-------------------- Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST
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THCybin
The Journey

Registered: 01/30/12
Posts: 129
Last seen: 11 years, 6 months
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Re: Help me [Re: zMan]
#17311252 - 11/30/12 07:28 PM (12 years, 1 month ago) |
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Ah. I really didn't want to build a still air box, but I don't want to ever encounter another jar of contaminants again either. I've considered it in the past but decided to see if I could get away without using one first, and from my results and advice given I guess I'll just break down and try to build a still air box, thanks again guys.
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zMan
zMan

Registered: 09/08/12
Posts: 2,191
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Quote:
THCybin said: Ah. I really didn't want to build a still air box, but I don't want to ever encounter another jar of contaminants again either. I've considered it in the past but decided to see if I could get away without using one first, and from my results and advice given I guess I'll just break down and try to build a still air box, thanks again guys.
For the price of 1 plastic clear tote ($8 dollars at wal mart for a big one) I think it'll be WELLLLLLLLLLLLLLL worth it.
-------------------- Information for everyone: http://www.mushroomvideos.com
Are your grains or spawn clean? CLICK THIS CHECKLIST
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Help me [Re: zMan] 1
#17316917 - 12/01/12 07:47 PM (12 years, 1 month ago) |
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Don't be cheap. Make a still air box. What it all boils down to is how badly you want mushrooms.
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THCybin
The Journey

Registered: 01/30/12
Posts: 129
Last seen: 11 years, 6 months
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Well I bought the still air box, I was going to get it anyways but the last straw for it was today. The very last jar that I thought was contaminant free developed a small green spot. The spot isn't any bigger than a "0" it literally is probably as big as that zero in quotations, it's only showing in one inoculation point that's why I don't think the rest of the white is from green mold but obviously for me to have been staring at the little dot it has to be a contaminant, I really want to keep it because it looks like the mycelium will just cover the spot but I don't want to be sick later but once again thanks for everything.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Help me [Re: THCybin] 1
#17318432 - 12/02/12 12:24 AM (12 years, 1 month ago) |
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That cake will not make it. The mold will not be eaten or go dormant. Just cut your losses, sterilize the cake, and toss it.
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THCybin
The Journey

Registered: 01/30/12
Posts: 129
Last seen: 11 years, 6 months
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I plan on doing it tomorrow just thought I'd report in, I really was hoping for a successful start and to share my journey on the forums but looks like I'll be having to wait for the 2nd attempt. I still have enough spores for one more jar, tomorrow I will attempt to start the last of el z strain and use the still air box with extreme caution and hopefully I get it right this time, if it's successful I'll be sure to let everyone know, the least I can do for all the help I received.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Help me [Re: THCybin] 1
#17318524 - 12/02/12 12:53 AM (12 years, 1 month ago) |
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Stick with it and continue improving your techniques.
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