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mashedglasses
Stranger


Registered: 04/06/11
Posts: 43
Last seen: 10 years, 8 months
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Contaminated Jar
#17307318 - 11/30/12 05:41 AM (12 years, 1 month ago) |
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http://imgur.com/a/kBwlr
Is my jar contaminated? It hasn't shown any change on this patch in 2 weeks (10-12 days I believe). I am guessing it is a bacterial contaminate but I am not as knowledgable as you folks.
Also is this jar unusable for anything if it is bacteria? Or could I take a grain and plop it on agar later on down the line?
Cheers for looking
Edited by mashedglasses (11/30/12 05:57 AM)
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JAB92
Stranger


Registered: 09/03/12
Posts: 304
Last seen: 3 years, 10 months
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i dont see any bacteria. i would take a pumped up bike tire and break up those grains let the mycillum grow %100 after that.
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DJEMBE
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creekfreek
Certified phunologist



Registered: 01/26/08
Posts: 4,818
Loc: Right about here
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Re: Contaminated Jar [Re: JAB92]
#17307559 - 11/30/12 07:17 AM (12 years, 1 month ago) |
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Im seeing what could be wet rot in these pics..... look at the creamy looking liquid around the uncolonized grains. Here is a good thread for seeing what good and bad look like. http://www.shroomery.org/forums/showflat.php/Number/17231150
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creekfreek
Certified phunologist



Registered: 01/26/08
Posts: 4,818
Loc: Right about here
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http://www.shroomery.org/5276/What-are-common-contaminants-of-the-mushroom-culture Here is a good contam page... notice the WETROT/sour spot pics... tho not as bad as these examples, your jars look like this.
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mashedglasses
Stranger


Registered: 04/06/11
Posts: 43
Last seen: 10 years, 8 months
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I believe so creekfreek. Would a shake do nothing beneficial for the mycelium to win the fight then?
Am I better off dumping the jar and sterilizing it in a PC?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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A shake is going to weaken your myc and the bacteria will flourish. Just shake it, open it, dump it, then wash with warm soapy water. You'll be PC'ing it when it's filled with your next batch of grains anyhow
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creekfreek
Certified phunologist



Registered: 01/26/08
Posts: 4,818
Loc: Right about here
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Yeah just dump that shit and try again... Its happened to me many times, I follow the WBS teks and I still wind up with some jars like this.
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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+1 to contaminates.
The myc seemed to do well with the rest of the jar, so no reason this patch should go unconquered, lest it be conquered already by bacteria of some sort.
Break up and dumpster tek.
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mashedglasses
Stranger


Registered: 04/06/11
Posts: 43
Last seen: 10 years, 8 months
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Re: Contaminated Jar [Re: Thadeous]
#17308469 - 11/30/12 11:27 AM (12 years, 1 month ago) |
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Cheers guys. I will dump and reuse it.
I didn't heat the water during the soaking of the grains before leaving it overnight to soak. I used cold tap water instead so that may have been a factor if the endospores need warm water during the soak to germinate.
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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That shouldn't matter, endospores are killed at temperatures and pressures reached during proper pressure cooking, there is no need for them to germinate.
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mashedglasses
Stranger


Registered: 04/06/11
Posts: 43
Last seen: 10 years, 8 months
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Re: Contaminated Jar [Re: Thadeous]
#17321353 - 12/02/12 03:12 PM (12 years, 1 month ago) |
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Oh I thought I read that the soak was to germinate temperature resistant endospores before frying them in the presure cookers heat. So soaking is to only hydrate the grains as all common endospores aren't resistant to the temperatures of a 15 PSI pressure cook for ~90 minutes?
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