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Offlinebig_balls
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Registered: 11/14/12
Posts: 125
Last seen: 12 years, 21 days
Re: glc [Re: thelanzii]
    #17306791 - 11/30/12 01:18 AM (12 years, 1 month ago)

Well with TS as well as a lot of other condescending people in the shroomery community making condescending assholish remarks I got offended. I am here because everytime I google something, this site pops up. If you can't answer a simple question with a straightforward answer and without bashing another person in a community like this? You're not a good person. You're a condescending asshole.
Now maybe I read that in the wrong tone of voice. Maybe psychoactive drugs have destroyed your ego making it impossible to communicate what you are saying without being condescending.
Either way I've been nothing but polite here. And yes this website has helped me but at the same time I've either had to deal with an asshole being condecwnding or someone treating this website like Nazi Germany in regards to certain forum rules..
Either way, thank you for your help. We are all adults here. Don't cry now.

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OfflineTrippy_Smurf
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Re: glc [Re: thelanzii]
    #17306796 - 11/30/12 01:20 AM (12 years, 1 month ago)

Quote:

Nemmies said:
whats the point in taking spore prints?



Spore prints can be made into 20-50 spore syringes.


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OfflinePussyFart
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Re: glc [Re: thelanzii]
    #17306810 - 11/30/12 01:26 AM (12 years, 1 month ago)

Quote:

Nemmies said:
whats the point in taking spore prints?



To collect the spores, to grow more mushrooms.

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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,435
Re: glc [Re: PussyFart]
    #17306829 - 11/30/12 01:33 AM (12 years, 1 month ago)

...

Edited by thelanzii (08/25/14 07:23 PM)

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OfflinePussyFart
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Re: glc [Re: thelanzii]
    #17306912 - 11/30/12 02:03 AM (12 years, 1 month ago)

Quote:

Nemmies said:
Quote:

Notahacker420 said:
Quote:

Nemmies said:
whats the point in taking spore prints?



To collect the spores, to grow more mushrooms.



so living culture spores out perform spore syringes from spore prints? also how long does a living culture last?



Living culture spores?

No, spore syringes are made from the spores that were collected via spore print.

Then you use this syringe to inoculate a substrate and let the spores germinate.

When they germinate, one spore mates with another spore to create a set of genetics.

These genetics express themselves as white mycellium, or a living culture if u will.

If you have a culture on agar it could last years in cold storage.

Spore prints can last way longer than cultures years if stored right.

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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,435
Re: glc [Re: PussyFart]
    #17306920 - 11/30/12 02:11 AM (12 years, 1 month ago)

...

Edited by thelanzii (08/25/14 07:23 PM)

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OfflinePussyFart
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Re: glc [Re: thelanzii]
    #17306972 - 11/30/12 02:36 AM (12 years, 1 month ago)

Quote:

Nemmies said:
so is a liquid culture syringe from spores the same thing as injected liquid into mycellium and then sucking it back up?



No...

When you have a fully colonized grain jar, it is easy to tell if the mycellium is clean or contaminated, and you make a GLC from it using that method, and you can be almost certain that its clean, granted the syringe and the water used were sterile.

But when u make an LC made from spores, test jars must be shot up and grown out to ensure its a clean culture.

LCs from spores are sketchy. No spore syringe/print is ever 100% clean.

This is because the cap from which the spores came was more than likely fruited in the open air.

You cannot know 100% if an LC is good or not by visual inspection.

Test jars must be shot up and grown out.

For best results, make LCs from a fully colonized grain jar(GLC) or from an agar wedge.

Or skip LC entirely and just G2G(Grain to Grain) transfers to expand your mycellium.

Edited by PussyFart (11/30/12 02:37 AM)

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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,435
Re: glc [Re: PussyFart]
    #17306997 - 11/30/12 03:00 AM (12 years, 1 month ago)

...

Edited by thelanzii (08/25/14 07:24 PM)

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Offlineshroom_bee
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Registered: 06/05/11
Posts: 293
Last seen: 7 months, 3 days
Re: glc [Re: thelanzii]
    #17307004 - 11/30/12 03:05 AM (12 years, 1 month ago)

Quote:

Nemmies said:
whats the point in taking spore prints?




Long term storage (myc goes bad).

Trading.  Prints are legal, all other stages of growth are not.

New genetics.  Sooner or later even of your myc doesn't contam, it will get "old" (every cell division introduces the possibility for mutation and degradation), and slow down. So even the perfect myc run needs to be replaced eventually.

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Offlineshroom_bee
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Registered: 06/05/11
Posts: 293
Last seen: 7 months, 3 days
Re: glc [Re: PussyFart]
    #17307035 - 11/30/12 03:27 AM (12 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

Nemmies said:
so is a liquid culture syringe from spores the same thing as injected liquid into mycellium and then sucking it back up?



No...

When you have a fully colonized grain jar, it is easy to tell if the mycellium is clean or contaminated, and you make a GLC from it using that method, and you can be almost certain that its clean, granted the syringe and the water used were sterile.

But when u make an LC made from spores, test jars must be shot up and grown out to ensure its a clean culture.

LCs from spores are sketchy. No spore syringe/print is ever 100% clean.

This is because the cap from which the spores came was more than likely fruited in the open air.

You cannot know 100% if an LC is good or not by visual inspection.

Test jars must be shot up and grown out.

For best results, make LCs from a fully colonized grain jar(GLC) or from an agar wedge.

Or skip LC entirely and just G2G(Grain to Grain) transfers to expand your mycellium.




Not arguing your logic, I prefer to use GLC, just:

From 3 vendors I received 24 needles over several orders.

In the 1st pass, 12 of those were used to noc up a variety of PF jars.
Out of those, 1 needle produced ALL contamed jars.  But it easily could have been my procedure back then.  The rest grew cleanly.

A year later, as I got more educated, I moved to straight rye jars. A dozen needles, all nocced directly, and then used for g2g. No contams.

A year later, I got more and more educated and used 4 needles to noc up 8 karo LC jars. All growth seems to revolve around the black spore groupings. From those, I noc rye and/or corn directly, and 8 LME/DEX as well.  Grew happily in both, no contams.  From the LME/DEX, I nocced up a BUNCH of corn quart jars.  More than 20, less than 200.

Not one contam from any of them with the exception of a bad PC run (too short, OLD OLD grain, my fault).  If I'm going to contam, it's gonna be tric in a tub. Which happens about 20% of the time. Still working on my clean room design.

In that same time frame I did a bunch of GLC.  It all worked fine, and it is certainly easier to have a few extra grain jars in a run to then use as opposed to take care of the LCs.  I do NOT lay them out after using them, I toss them.  That extra water sliming up the jar gives me the willies at that point.

I also did a bunch of clones->LC in the process, but I'll ignore that for now.

On the other hand, GLC means using the next generation again and again.  This means the myc is getting older very fast, as compared to 2 needles away from my original Spores->Karo LC-> Malt/Dex LC->grain jar. So as long as the original karo jars stays good (it stalled nicely), I have the ability to generate a fresh multispore jars or malt/dex LC very fast.

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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,435
Re: glc [Re: big_balls]
    #17307076 - 11/30/12 03:53 AM (12 years, 1 month ago)

..

Edited by thelanzii (08/25/14 07:24 PM)

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Offlineshroom_bee
Stranger
Registered: 06/05/11
Posts: 293
Last seen: 7 months, 3 days
Re: glc [Re: big_balls]
    #17307096 - 11/30/12 04:06 AM (12 years, 1 month ago)

Quote:

big_balls said:
Well with TS as well as a lot of other condescending people in the shroomery community making condescending assholish remarks I got offended. I am here because everytime I google something, this site pops up. If you can't answer a simple question with a straightforward answer and without bashing another person in a community like this? You're not a good person. You're a condescending asshole.
Now maybe I read that in the wrong tone of voice. Maybe psychoactive drugs have destroyed your ego making it impossible to communicate what you are saying without being condescending.
Either way I've been nothing but polite here. And yes this website has helped me but at the same time I've either had to deal with an asshole being condecwnding or someone treating this website like Nazi Germany in regards to certain forum rules..
Either way, thank you for your help. We are all adults here. Don't cry now.




Sounds like a Monty Python skit.  Your offense has offended me. I've been offended, I must strike back!

Speaking of rules:  Godwin.
http://en.wikipedia.org/wiki/Godwin's_law
You lose the internet game.
Thank you for playing.

Note:  I could work on my condescension levels, but something tells me they would be wasted on you.

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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: glc [Re: shroom_bee]
    #17307098 - 11/30/12 04:11 AM (12 years, 1 month ago)

Quote:

shroom_bee said:
On the other hand, GLC means using the next generation again and again.




What do u mean by this?

A generation is from spore to fruit, now if u take a tissue sample from this fruit and colonize more jars and then fruit those, that would be generation 2.(to my knowledge)

A grain to grain transfer is not considered a generation.

Edited by PussyFart (11/30/12 04:12 AM)

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Offlineshroom_bee
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Registered: 06/05/11
Posts: 293
Last seen: 7 months, 3 days
Re: glc [Re: PussyFart]
    #17307142 - 11/30/12 04:33 AM (12 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

shroom_bee said:
On the other hand, GLC means using the next generation again and again.




What do u mean by this?

A generation is from spore to fruit, now if u take a tissue sample from this fruit and colonize more jars and then fruit those, that would be generation 2.(to my knowledge)

A grain to grain transfer is not considered a generation.




Maybe my terminology is off. However long myc is alive, if it is in active growth, it is aging. Aging is due to accumulated mutations triggered in each cell division.  Aging can be mitigated by giving myc different things to eat, so each transfer might have a bit of different mix of nutrients, but the bottom line is it gets old, sooner or later, and senescence kicks in.

This is indicated by slow growth and poor fruiting at that point.

I termed (possibly incorrectly) this as multiple generations out, ie: for each transfer into a new medium, new generation is triggered.  But it is certainly not a fruiting to spore generation.

So, regardless of the terminology (feel free to give me the correct words for the description), as myc gets older, and is 'x' levels (not generations, then what) from the original multispore base, it gets old and occasionally needs to be renewed from something a bit closer to the original spore base.

Or am I myth repeating?

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