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big_balls
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glc
#17305348 - 11/29/12 07:58 PM (12 years, 1 month ago) |
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So my glc has this like "clear goo layer" Around the grains. Its 4 days in. Is this my myc. I knocked up another lc with spores and so far nothing. I can see some super tiny red dots floating around in both. What's this..
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PussyFart
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GLC is the way to go vs. LCs from spores. LCs from spores suck.
Pics would help.
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A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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big_balls
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I have a pic. But It won't let me upload it to here..
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Beefy1
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Quote:
big_balls said: So my glc has this like "clear goo layer" Around the grains.
Um, your GLC has grain in it?
Should just be water with myc in it.
Goop around the grains sounds ike bacteria. That would happen if you just added water to a jar of grain and left it to sit.
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big_balls
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I'll poop on you.
Edited by big_balls (12/01/12 06:02 AM)
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big_balls
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You can very slightly see clear growth around the grain. I'm hoping it myc cuz I cant Afford another syringe since 101 fucked me. What am I supposed to. Dunk the colonized grain in the lc and pull it out? Lol
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Beefy1
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What procedure did you follow for making that?
Could be mushroom myc. could be mold.
Only way to find out would be to knock up a jar with it and try to grow it out.
It's most likely contaminated.
To make a GLC
Start with a Healthy Fully Colonized jar of grain.
Inject sterile water into jar of grain.
Shake.
Suck water/myc mixture back into syringe.
Use like any other syringe of LC. No waiting.
Let your grain jar re-colonize. Use it as spawn
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PussyFart
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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big_balls
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Yeah. I didn't like the thought of shooting water into my colonized jar. Also I didn't wanna shake the jar again. I was birthing it. The. Next day 1tsp honey to every 200ml water PCd for 20 minutes. Did grain transfer in a still air box.
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PussyFart
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You cannot know 100% if an LC is good or not by visual inspection.
Test jars must be shot up and grown out.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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big_balls
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Yeah I know this I just was wondering if you have seen this before.
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Beefy1
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Quote:
big_balls said: Yeah. I didn't like the thought of shooting water into my colonized jar. Also I didn't wanna shake the jar again. I was birthing it. The. Next day 1tsp honey to every 200ml water PCd for 20 minutes. Did grain transfer in a still air box.
Alright, I see what you did now. You just made an LC and inoculated it with a piece of grain. If your grain was clean and healthy to begin with you could still be good. Give it a few more days to thicken up then make some test jars with it.
GLC generally refers to just Myc suspended in water. No sugar.
Contams love sugar water.
I've never actually seen a person just drop a piece of popcorn in an LC.
Only one way to find out if it worked.
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big_balls
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Yeah the grains were fine. And my method was sterile. I have a spare jar of grains so I'll knock that up in a few days. I'm. Confused on the meaning of glc then. You're. Talking about shooting sterile. Water. Into. The grain. And sucking it back up right. Sorry for. All the. ......... my phones gaaaaay
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PussyFart
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Yes, shoot it in a fully colonized grain jar...shake it up...suck it out
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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Trippy_Smurf
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Quote:
Notahacker420 said: Yes, shoot it in a fully colonized grain jar...shake it up...suck it out

Seriously? You opened a sterile jar (thus making it unsterile), and put it in an LC, which you had to open, thus making it also unsterile? And you did all this because you didn't want to do what several experienced growers with lots of success said to do?
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Kids: There's a time and a place for everything, and it's called college.
How to be a good shroomie How to grow mushrooms A collection of good links (may be outdated) How things should look How to pass a drug test
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big_balls
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Yeah sorry but compared to my method that sounds like a stupid fucking idea.
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PussyFart
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Quote:
big_balls said: Yeah sorry but compared to my method that sounds like a stupid fucking idea.
That's the attitude to have!
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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Trippy_Smurf
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Quote:
big_balls said: Yeah sorry but compared to my method that sounds like a stupid fucking idea.
Then why are you here?
-------------------- SECURITY: READ THIS!
Chef: Kids, what did I tell you about drugs?
Kids: There's a time and a place for everything, and it's called college.
How to be a good shroomie How to grow mushrooms A collection of good links (may be outdated) How things should look How to pass a drug test
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Beefy1
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Quote:
Notahacker420 said: Yes, shoot it in a fully colonized grain jar...shake it up...suck it out
GLC on this site refers to this.
Use a big syringe. you knock myc off of the colonized grains into the water, end up with all the LC you need.
What OP made is just called LC around here. Many people do not like it.
They feel that a jar of sugar water that you have to add something to and then wait a week for it to grow is just asking for a problem.
It seems much better to start off with clean water that already has all of the myc you need in it. No waiting. Less chance of contamination.
Good luck whatever you want to do OP.
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thelanzii

Registered: 11/13/12
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whats the point in taking spore prints?
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big_balls
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Well with TS as well as a lot of other condescending people in the shroomery community making condescending assholish remarks I got offended. I am here because everytime I google something, this site pops up. If you can't answer a simple question with a straightforward answer and without bashing another person in a community like this? You're not a good person. You're a condescending asshole. Now maybe I read that in the wrong tone of voice. Maybe psychoactive drugs have destroyed your ego making it impossible to communicate what you are saying without being condescending. Either way I've been nothing but polite here. And yes this website has helped me but at the same time I've either had to deal with an asshole being condecwnding or someone treating this website like Nazi Germany in regards to certain forum rules.. Either way, thank you for your help. We are all adults here. Don't cry now.
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Trippy_Smurf
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Quote:
Nemmies said: whats the point in taking spore prints?
Spore prints can be made into 20-50 spore syringes.
-------------------- SECURITY: READ THIS!
Chef: Kids, what did I tell you about drugs?
Kids: There's a time and a place for everything, and it's called college.
How to be a good shroomie How to grow mushrooms A collection of good links (may be outdated) How things should look How to pass a drug test
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PussyFart
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Quote:
Nemmies said: whats the point in taking spore prints?
To collect the spores, to grow more mushrooms.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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thelanzii

Registered: 11/13/12
Posts: 5,435
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...
Edited by thelanzii (08/25/14 07:23 PM)
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PussyFart
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Quote:
Nemmies said:
Quote:
Notahacker420 said:
Quote:
Nemmies said: whats the point in taking spore prints?
To collect the spores, to grow more mushrooms.
so living culture spores out perform spore syringes from spore prints? also how long does a living culture last?
Living culture spores?
No, spore syringes are made from the spores that were collected via spore print.
Then you use this syringe to inoculate a substrate and let the spores germinate.
When they germinate, one spore mates with another spore to create a set of genetics.
These genetics express themselves as white mycellium, or a living culture if u will.
If you have a culture on agar it could last years in cold storage.
Spore prints can last way longer than cultures years if stored right.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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thelanzii

Registered: 11/13/12
Posts: 5,435
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...
Edited by thelanzii (08/25/14 07:23 PM)
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PussyFart
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Quote:
Nemmies said: so is a liquid culture syringe from spores the same thing as injected liquid into mycellium and then sucking it back up?
No...
When you have a fully colonized grain jar, it is easy to tell if the mycellium is clean or contaminated, and you make a GLC from it using that method, and you can be almost certain that its clean, granted the syringe and the water used were sterile.
But when u make an LC made from spores, test jars must be shot up and grown out to ensure its a clean culture.
LCs from spores are sketchy. No spore syringe/print is ever 100% clean.
This is because the cap from which the spores came was more than likely fruited in the open air.
You cannot know 100% if an LC is good or not by visual inspection.
Test jars must be shot up and grown out.
For best results, make LCs from a fully colonized grain jar(GLC) or from an agar wedge.
Or skip LC entirely and just G2G(Grain to Grain) transfers to expand your mycellium.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/12 02:37 AM)
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thelanzii

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...
Edited by thelanzii (08/25/14 07:24 PM)
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shroom_bee
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Quote:
Nemmies said: whats the point in taking spore prints?
Long term storage (myc goes bad).
Trading. Prints are legal, all other stages of growth are not.
New genetics. Sooner or later even of your myc doesn't contam, it will get "old" (every cell division introduces the possibility for mutation and degradation), and slow down. So even the perfect myc run needs to be replaced eventually.
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shroom_bee
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Quote:
Notahacker420 said:
Quote:
Nemmies said: so is a liquid culture syringe from spores the same thing as injected liquid into mycellium and then sucking it back up?
No...
When you have a fully colonized grain jar, it is easy to tell if the mycellium is clean or contaminated, and you make a GLC from it using that method, and you can be almost certain that its clean, granted the syringe and the water used were sterile.
But when u make an LC made from spores, test jars must be shot up and grown out to ensure its a clean culture.
LCs from spores are sketchy. No spore syringe/print is ever 100% clean.
This is because the cap from which the spores came was more than likely fruited in the open air.
You cannot know 100% if an LC is good or not by visual inspection.
Test jars must be shot up and grown out.
For best results, make LCs from a fully colonized grain jar(GLC) or from an agar wedge.
Or skip LC entirely and just G2G(Grain to Grain) transfers to expand your mycellium.
Not arguing your logic, I prefer to use GLC, just:
From 3 vendors I received 24 needles over several orders.
In the 1st pass, 12 of those were used to noc up a variety of PF jars. Out of those, 1 needle produced ALL contamed jars. But it easily could have been my procedure back then. The rest grew cleanly.
A year later, as I got more educated, I moved to straight rye jars. A dozen needles, all nocced directly, and then used for g2g. No contams.
A year later, I got more and more educated and used 4 needles to noc up 8 karo LC jars. All growth seems to revolve around the black spore groupings. From those, I noc rye and/or corn directly, and 8 LME/DEX as well. Grew happily in both, no contams. From the LME/DEX, I nocced up a BUNCH of corn quart jars. More than 20, less than 200.
Not one contam from any of them with the exception of a bad PC run (too short, OLD OLD grain, my fault). If I'm going to contam, it's gonna be tric in a tub. Which happens about 20% of the time. Still working on my clean room design.
In that same time frame I did a bunch of GLC. It all worked fine, and it is certainly easier to have a few extra grain jars in a run to then use as opposed to take care of the LCs. I do NOT lay them out after using them, I toss them. That extra water sliming up the jar gives me the willies at that point.
I also did a bunch of clones->LC in the process, but I'll ignore that for now.
On the other hand, GLC means using the next generation again and again. This means the myc is getting older very fast, as compared to 2 needles away from my original Spores->Karo LC-> Malt/Dex LC->grain jar. So as long as the original karo jars stays good (it stalled nicely), I have the ability to generate a fresh multispore jars or malt/dex LC very fast.
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thelanzii

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..
Edited by thelanzii (08/25/14 07:24 PM)
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shroom_bee
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Quote:
big_balls said: Well with TS as well as a lot of other condescending people in the shroomery community making condescending assholish remarks I got offended. I am here because everytime I google something, this site pops up. If you can't answer a simple question with a straightforward answer and without bashing another person in a community like this? You're not a good person. You're a condescending asshole. Now maybe I read that in the wrong tone of voice. Maybe psychoactive drugs have destroyed your ego making it impossible to communicate what you are saying without being condescending. Either way I've been nothing but polite here. And yes this website has helped me but at the same time I've either had to deal with an asshole being condecwnding or someone treating this website like Nazi Germany in regards to certain forum rules.. Either way, thank you for your help. We are all adults here. Don't cry now.
Sounds like a Monty Python skit. Your offense has offended me. I've been offended, I must strike back!
Speaking of rules: Godwin. http://en.wikipedia.org/wiki/Godwin's_law You lose the internet game. Thank you for playing.
Note: I could work on my condescension levels, but something tells me they would be wasted on you.
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PussyFart
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Quote:
shroom_bee said: On the other hand, GLC means using the next generation again and again.
What do u mean by this?
A generation is from spore to fruit, now if u take a tissue sample from this fruit and colonize more jars and then fruit those, that would be generation 2.(to my knowledge)
A grain to grain transfer is not considered a generation.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/12 04:12 AM)
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shroom_bee
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Quote:
Notahacker420 said:
Quote:
shroom_bee said: On the other hand, GLC means using the next generation again and again.
What do u mean by this?
A generation is from spore to fruit, now if u take a tissue sample from this fruit and colonize more jars and then fruit those, that would be generation 2.(to my knowledge)
A grain to grain transfer is not considered a generation.
Maybe my terminology is off. However long myc is alive, if it is in active growth, it is aging. Aging is due to accumulated mutations triggered in each cell division. Aging can be mitigated by giving myc different things to eat, so each transfer might have a bit of different mix of nutrients, but the bottom line is it gets old, sooner or later, and senescence kicks in.
This is indicated by slow growth and poor fruiting at that point.
I termed (possibly incorrectly) this as multiple generations out, ie: for each transfer into a new medium, new generation is triggered. But it is certainly not a fruiting to spore generation.
So, regardless of the terminology (feel free to give me the correct words for the description), as myc gets older, and is 'x' levels (not generations, then what) from the original multispore base, it gets old and occasionally needs to be renewed from something a bit closer to the original spore base.
Or am I myth repeating?
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