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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Last seen: 7 months, 28 days
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**Votes Needed** AGAR TRANSFER POLL!!!**Votes Needed**
#17294521 - 11/27/12 11:30 PM (12 years, 1 month ago) |
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Just curious to see how people do things.
Edited by PussyFart (11/29/12 01:40 PM)
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,285
Loc: N38.93829W119.98108
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Re: AGAR TRANSFER POLL!!! [Re: PussyFart]
#17295187 - 11/28/12 02:27 AM (12 years, 1 month ago) |
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There are reasons for placing the tranfers in certain ways. If It is a transfer from a dirt plate, I place the mycelium facing down just in case to help keep the bad stuff trapped. This is usally done with antibiotic agar. I cut in both squares and triangles depending on if I like the growth or not. Not that it changes the over all outcome but it does change the rate of the initial new growth. I dont sterilize between transfers off the same dish. I mainly flip the over just because of the way I stab them. The mycelium doesnt only grow on top of the agar. Its in all of it
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987
Teh=The
I need to proofread
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PussyFart
Retired Cultivation Extrodinaire



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Re: AGAR TRANSFER POLL!!! [Re: tahoe]
#17297960 - 11/28/12 03:37 PM (12 years, 1 month ago) |
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I don't sterilize between transfers from the same dish either.
But I do have a couple dishes where the wedge was placed facing down over a week ago and they show no growth period.
This is what made me start this poll.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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PocketRevolution
Stranger
Registered: 12/22/09
Posts: 270
Last seen: 7 months, 27 days
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Re: AGAR TRANSFER POLL!!! [Re: PussyFart]
#17298126 - 11/28/12 04:01 PM (12 years, 1 month ago) |
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I chose "flame sterilize" to mean "use a new pre-sterilized tool"
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Quote:
PocketRevolution said: I chose "flame sterilize" to mean "use a new pre-sterilized tool"
If u do that between each dish, you must be made of money.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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Revolutiontcm
dniM-retsaM



Registered: 04/05/12
Posts: 949
Loc: MD
Last seen: 4 months, 11 days
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Lol, i voted nice poll...
Too funny
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11111
Stranger

Registered: 12/16/11
Posts: 120
Last seen: 7 years, 3 days
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goto start doing triangles... less cuts.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: AGAR TRANSFER POLL!!! [Re: 11111]
#17303238 - 11/29/12 01:40 PM (12 years, 1 month ago) |
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Come on people, I need your votes! 90 views and 16 votes is unacceptable!
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/29/12 01:41 PM)
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Destitute
floss daily
Registered: 11/28/12
Posts: 4,027
Loc: where
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Re: AGAR TRANSFER POLL!!! [Re: PussyFart]
#17303280 - 11/29/12 01:47 PM (12 years, 1 month ago) |
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Sorry I don't do agar... yet!!
-------------------- [quote]Prisoner#1 said:
I've done your mother's crack[/quote]
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: AGAR TRANSFER POLL!!! [Re: Destitute]
#17303383 - 11/29/12 02:06 PM (12 years, 1 month ago) |
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I, normally, place the transfer facing up or on its side. It just depends on how the cut is. I don't cut in squares or triangles. I cut in rectangles.  I don't flame between transfer from the same dish. I get free sterile blades from another shroomery member, so I normally just use a new sterile blade between dishes.
& sorry, it wasn't a decent poll, because no STAL option was available.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Quote:
TranscendingLife said: & sorry, it wasn't a decent poll, because no STAL option was available.
Yea, WTF does that mean anyways?...could never figure that out.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: AGAR TRANSFER POLL!!! [Re: PussyFart]
#17303427 - 11/29/12 02:15 PM (12 years, 1 month ago) |
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It's an old OTD/Pubber. I'm not sure exactly how he managed to get into every poll, but it's not a legit poll on here unless there's the option to STAL.
Found it: https://www.shroomery.org/forums/showflat.php/Number/11362626#11362626
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Quote:
TranscendingLife said: It's an old OTD/Pubber. I'm not sure exactly how he managed to get into every poll, but it's not a legit poll on here unless there's the option to STAL.
Found it: https://www.shroomery.org/forums/showflat.php/Number/11362626#11362626
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: AGAR TRANSFER POLL!!! [Re: PussyFart]
#17303472 - 11/29/12 02:22 PM (12 years, 1 month ago) |
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it is what is is... Now some agar pictures. Pin on agar (after transfer):

Agar wedge colonizing rye grains:
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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Zarotti
Stranger

Registered: 05/12/12
Posts: 314
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What is the fastest way of colonizing your grain jars ?
Is this the right order :
fastest
1.g2g 2.agar piece ... spore syringe spore print ...
slowest
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BjJiggles
Gym seeker



Registered: 03/13/12
Posts: 2,737
Loc:
Last seen: 3 months, 7 days
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Re: AGAR TRANSFER POLL!!! [Re: Zarotti]
#17303886 - 11/29/12 03:31 PM (12 years, 1 month ago) |
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Well G2G doesn't really fit into the group... You would use a spore syringe or and agar piece"wedge" to inoculate your grains, then when your master jars are fully colonized you g2g those out... You can't really start w g2g or compare speeds with agar wedges...
That being said, g2g is by far the fastest way to expand your myc... I usually inoculate with an agar wedge then g2g the master jars out when they are fully colonized.... I've never done spore syringe straight to grain, since I like agar, and your forgot LC to grains, which is probably the fastest, but sometimes LC's can get you in trouble if your not careful....
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Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: AGAR TRANSFER POLL!!! [Re: BjJiggles]
#17304598 - 11/29/12 05:41 PM (12 years, 1 month ago) |
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I actually ordered my 1st petri and parafilm this week. I'll vote latter.
I just want to track this thread because i have an ass-load of questions.
-------------------- The Basics
A little civility goes a long way
The Noob Forum
The Hammock Hangers' Forum
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 2 years, 3 months
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Instead of triangles I cut medial triambic icosahedron's, face up, I flame sterilize between every cut. Awesome poll.
-------------------- ¯\_(ツ)_/¯
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PussyFart
Retired Cultivation Extrodinaire



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Posts: 22,502
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Last seen: 7 months, 28 days
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Re: AGAR TRANSFER POLL!!! [Re: Tmethyl]
#17304759 - 11/29/12 06:08 PM (12 years, 1 month ago) |
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Quote:
Tmethyl said: Instead of triangles I cut medial triambic icosahedron's, face up
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 2 years, 3 months
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Re: AGAR TRANSFER POLL!!! [Re: PussyFart]
#17304773 - 11/29/12 06:10 PM (12 years, 1 month ago) |
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-------------------- ¯\_(ツ)_/¯
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