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Offlinetoneboy
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Will this tyvek suit work for a grain lid?
    #17301492 - 11/29/12 06:04 AM (12 years, 1 month ago)

I have access to this suit:

http://www.northernsafety.com/Product/4402/DuPont-Tyvek-Disposable-Lab-Coat-No-Pockets

Will this work for MYC projects specifically for a Grain in Quart Jar lid?

Any Links to a Grain to Quart Jar LID Tek?

Found One that has the tyvek and the band only. Doubtful about it since I think that would dry out my grains.

Thanks In Advance!

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Offlinetoneboy
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17305507 - 11/29/12 08:29 PM (12 years, 1 month ago)

Anyone?

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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17305517 - 11/29/12 08:31 PM (12 years, 1 month ago)

Yea, that should work...

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Offlineboneynerd
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Re: Will this tyvek suit work for a grain lid? [Re: PussyFart]
    #17306933 - 11/30/12 02:17 AM (12 years, 1 month ago)

Thats what I did just bought a tyvek painter suit from like lowes or home depot and cut it into the pieces you need.


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Invisibleserotonin storm
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Re: Will this tyvek suit work for a grain lid? [Re: boneynerd]
    #17306939 - 11/30/12 02:20 AM (12 years, 1 month ago)

If you did quarter sized filters, that coat would last 1000 jars


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Offlinecolax03
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Re: Will this tyvek suit work for a grain lid? [Re: serotonin storm]
    #17307351 - 11/30/12 05:56 AM (12 years, 1 month ago)

if youre in the US you can always just grab priority mail envelopes for free

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OfflinePussyFart
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Re: Will this tyvek suit work for a grain lid? [Re: colax03]
    #17307390 - 11/30/12 06:11 AM (12 years, 1 month ago)

Quote:

colax03 said:
if youre in the US you can always just grab priority mail envelopes for free



PO tyvek is not as good of filter material as paint suit tyvek.

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InvisibleFrankHorrigan
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Re: Will this tyvek suit work for a grain lid? [Re: PussyFart]
    #17307551 - 11/30/12 07:15 AM (12 years, 1 month ago)

If it says "DuPont Tyvek" it's good to go. If you're concerned, use two layers for your filter :thumbup:


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OfflineXFINITY
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Re: Will this tyvek suit work for a grain lid? [Re: FrankHorrigan]
    #17307969 - 11/30/12 09:40 AM (12 years, 1 month ago)

If you have free access to this type of Tyvek suit then go for it, if you are paying for them I would suggest using a more economical source.

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Offlinetabiyeh
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Re: Will this tyvek suit work for a grain lid? *DELETED* [Re: PussyFart]
    #17308068 - 11/30/12 10:10 AM (12 years, 1 month ago)

Post deleted by tabiyeh

Reason for deletion: Life is lowly anonymity.


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OfflineClever_You
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17308072 - 11/30/12 10:12 AM (12 years, 1 month ago)

i figure if a guy is gonna buy a tyvek coverall might as well order some filter disk....i got tyvek envlopes and tyvek coveralls and both seem to be working great so far...

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OfflineTrippy_Smurf
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Re: Will this tyvek suit work for a grain lid? [Re: tabiyeh]
    #17308087 - 11/30/12 10:14 AM (12 years, 1 month ago)

Tyvek is the name brand for a polyethylene-based material.  If it's actually tyvek, it's made by Dupont, and it's good.  However, there's many different manufacturers of the same type of material that are of lower quality.

Also, there are several different types of Dupont Tyvek, and only some are suitable for filters.  Painters suit tyvek is great.  House wrap is not.


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InvisibleFrankHorrigan
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Re: Will this tyvek suit work for a grain lid? [Re: Trippy_Smurf]
    #17308110 - 11/30/12 10:22 AM (12 years, 1 month ago)

Quote:

Trippy_Smurf said:
Tyvek is the name brand for a polyethylene-based material.  If it's actually tyvek, it's made by Dupont, and it's good.  However, there's many different manufacturers of the same type of material that are of lower quality.

Also, there are several different types of Dupont Tyvek, and only some are suitable for filters.  Painters suit tyvek is great.  House wrap is not.




:whathesaid:


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Offlinetoneboy
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Re: Will this tyvek suit work for a grain lid? [Re: FrankHorrigan]
    #17310406 - 11/30/12 05:11 PM (12 years, 1 month ago)

Theres a surplus shop here that sells it for two dollars so Im good with the cost. Im not from the US though.

Saw a tek wherein they cut the tyvek in round pieces and put it on top of a 3/8" inch hole on top of the grain lids and glued it down with silicone.

If I do this do I still double up the tyvek?
Would that hole size be okay?

Any proven tyvek with injection port grain lid teks out there?

Thanks

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Offlinetoneboy
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17318559 - 12/02/12 01:05 AM (12 years, 1 month ago)

Do i put the tyvek outside the grain lid or under a ge hole?
Thanks

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Offlinec0ri
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17318658 - 12/02/12 01:40 AM (12 years, 1 month ago)

Tyvek Envelopes > Polyfil > Tyvek Suits > Micropore Tape > SFD's

Atleast in my experience.

I've used all, and had pretty much the same results from the envelopes up to the suits. Tape worked great, and SFD's just own all.

In MY experience.

---

To answer your question, some people put the tyvek down and then the lid, some put the lid and then the tyvek. I prefer lid and then tyvek.

Edited by c0ri (12/02/12 01:42 AM)

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InvisibleFischer
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17319099 - 12/02/12 06:11 AM (12 years, 1 month ago)

Quote:

c0ri said:
Tyvek Envelopes > Polyfil > Tyvek Suits > Micropore Tape > SFD's




For clarification, your arrows are round the wrong way :wink:

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Offlinetoneboy
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17319713 - 12/02/12 10:08 AM (12 years, 1 month ago)

Quote:

c0ri said:
Tyvek Envelopes > Polyfil > Tyvek Suits > Micropore Tape > SFD's

Atleast in my experience.

I've used all, and had pretty much the same results from the envelopes up to the suits. Tape worked great, and SFD's just own all.

In MY experience.

---

To answer your question, some people put the tyvek down and then the lid, some put the lid and then the tyvek. I prefer lid and then tyvek.




If the tyvek covers the lid would you inject thru the tyvek?

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InvisibleFrankHorrigan
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17319809 - 12/02/12 10:30 AM (12 years, 1 month ago)

Quote:

toneboy said:
If the tyvek covers the lid would you inject thru the tyvek?




You would make a self-healing inoc port with RTV silicone. Then you could inoc through there and through the tyvek under it.


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


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OfflineGoldenArrow
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17319875 - 12/02/12 10:42 AM (12 years, 1 month ago)

Quote:

toneboy said:
Saw a tek wherein they cut the tyvek in round pieces and put it on top of a 3/8" inch hole on top of the grain lids and glued it down with silicone.

If I do this do I still double up the tyvek?
Would that hole size be okay?

Do i put the tyvek outside the grain lid or under a ge hole?





What I would do along those lines is do 4 or 5 smaller holes, and a circle of tyvek over the top. This way you can shake the jar and the tyvek won't get touched/wet.

What I actually do these days is very very tight polyfil through a hole in the lid, then a 'belt and braces' approach of putting a circle of tyvek siliconed over the whole lid once it's inoculated. This way I don't have to start peeling off old tyvek to change it every so many number of uses.

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OfflineGoldenArrow
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17319882 - 12/02/12 10:44 AM (12 years, 1 month ago)

Quote:

c0ri said:
Tyvek Envelopes > Polyfil > Tyvek Suits > Micropore Tape > SFD's




Very interesting that you rate micropore above tyvek, I've seen a lot of people stating it's totally useless! I've started using it for a half-assed GE port on my LCs (double layer) so am happy that's not the case :smile:

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Offlinec0ri
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Re: Will this tyvek suit work for a grain lid? [Re: GoldenArrow]
    #17324282 - 12/03/12 12:08 AM (12 years, 1 month ago)

I generally use sfd's but when i run out I use 2 layers of micropore tape. Not sure why people would say it sucks, cuz i've never had a single contam in a jar using micropore tape. The only contams ive had were using polyfil and using a tyvek paint suit.

Had better luck with envelopes than with the suits everyone says are better.

Just my experience.

Oh forgot to mention I typically see faster colonization times using 2 layers of micropore than I do with sfd's. Can't explain it.

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OfflinePussyFart
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17324294 - 12/03/12 12:10 AM (12 years, 1 month ago)

Quote:

c0ri said:
Oh forgot to mention I typically see faster colonization times using 2 layers of micropore than I do with sfd's. Can't explain it.




Has your experience been with MS or with isolated monocultures?

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InvisibleFrankHorrigan
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Re: Will this tyvek suit work for a grain lid? [Re: PussyFart]
    #17324302 - 12/03/12 12:12 AM (12 years, 1 month ago)

Quote:

Notahacker420 said:

Has your experience been with MS or with isolated monocultures?




Has yours? :crazy2:


--------------------

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You should take a look. :hehehe:


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AMU- Get an answer here -AMU

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Offlinec0ri
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Re: Will this tyvek suit work for a grain lid? [Re: FrankHorrigan]
    #17324312 - 12/03/12 12:15 AM (12 years, 1 month ago)

with both actually. ms I typically just put 1 layer for the pc and then noc with the syringe and place another over the first. with agar i just use it as a filter and it works just fine.

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InvisibleFrankHorrigan
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17324342 - 12/03/12 12:20 AM (12 years, 1 month ago)

Quote:

c0ri said:
with both actually. ms I typically just put 1 layer for the pc and then noc with the syringe and place another over the first. with agar i just use it as a filter and it works just fine.




...this fucking guy... :facepalm3:

I'm on to you.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Edited by FrankHorrigan (12/03/12 12:48 AM)

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OfflinePussyFart
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Re: Will this tyvek suit work for a grain lid? [Re: FrankHorrigan]
    #17324539 - 12/03/12 01:06 AM (12 years, 1 month ago)

Quote:

FrankHorrigan said:
Quote:

Notahacker420 said:

Has your experience been with MS or with isolated monocultures?




Has yours? :crazy2:



Lately....:bigyesnod:....isolates!

Edited by PussyFart (12/03/12 01:06 AM)

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Offlinec0ri
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Re: Will this tyvek suit work for a grain lid? [Re: FrankHorrigan]
    #17324660 - 12/03/12 01:51 AM (12 years, 1 month ago)

Quote:

FrankHorrigan said:
Quote:

c0ri said:
with both actually. ms I typically just put 1 layer for the pc and then noc with the syringe and place another over the first. with agar i just use it as a filter and it works just fine.




...this fucking guy... :facepalm3:

I'm on to you.




What exactly are you onto there buddy?

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Offlinec0ri
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17324672 - 12/03/12 01:56 AM (12 years, 1 month ago)



You caught me! None of this is real!



Or this!



and Especially this!



Get off my :casket:

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OfflinePussyFart
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17324739 - 12/03/12 02:23 AM (12 years, 1 month ago)

Got any pics of your isolates on agar?

I'm a fan of rhizos!

Edited by PussyFart (12/03/12 02:23 AM)

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Offlinetoneboy
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Re: Will this tyvek suit work for a grain lid? [Re: PussyFart]
    #17325065 - 12/03/12 05:31 AM (12 years, 1 month ago)

Im planning on doing grains. Isn't the 4 hole GE for BRF jars?

If I cut up the tyvek in quarter sizes how can I "double up"?

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OfflinePussyFart
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17325114 - 12/03/12 06:00 AM (12 years, 1 month ago)

Quote:

toneboy said:
Im planning on doing grains. Isn't the 4 hole GE for BRF jars?

If I cut up the tyvek in quarter sizes how can I "double up"?



By placing a layer on top and the bottom of the lid.

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OfflineGoldenArrow
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Re: Will this tyvek suit work for a grain lid? [Re: toneboy]
    #17325145 - 12/03/12 06:15 AM (12 years, 1 month ago)

Quote:

toneboy said:
Im planning on doing grains. Isn't the 4 hole GE for BRF jars?




There are no rules, I'm not talking about 4 holes  at the edges a la pf tek, I'm talking about 4 or 5 virtually touching like:

x x
x x

In the middle of the lid, in order to stop wet grains from directly touching the tyvek.

And yep, top and bottom or just stick 2 layers together. As I said, that seems too messy for me so I've moved to polyfil directly on the lid and tyvek elastic banded over the top.

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InvisibleFrankHorrigan
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Re: Will this tyvek suit work for a grain lid? [Re: c0ri]
    #17325406 - 12/03/12 08:12 AM (12 years, 1 month ago)

Quote:

c0ri said:
with both actually. ms I typically just put 1 layer for the pc and then noc with the syringe and place another over the first. with agar i just use it as a filter and it works just fine.




Quote:

c0ri said:









:laugh2:
Nice agar work there cori...oh wait, all I see are a bunch of shitty looking LCs :lol:
But if we're playing the show-off game, I've got you beat already:


Shame he won't see it without his puppet accounts, he's banned :lol:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Edited by FrankHorrigan (12/03/12 08:36 AM)

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InvisibleFrankHorrigan
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Re: Will this tyvek suit work for a grain lid? [Re: PussyFart]
    #17325458 - 12/03/12 08:25 AM (12 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

FrankHorrigan said:
Quote:

Notahacker420 said:

Has your experience been with MS or with isolated monocultures?




Has yours? :crazy2:



Lately....:bigyesnod:....isolates!




Me too, I just knocked up 24 master jars with PE isolates I've been working on for a couple months.  Average of six transfers to monoculture.
Agar is great.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflineTrippy_Smurf
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Re: Will this tyvek suit work for a grain lid? [Re: FrankHorrigan]
    #17325617 - 12/03/12 09:12 AM (12 years, 1 month ago)

Quote:

FrankHorrigan said:






Nice set up.  Looks like my guest bedroom.  Wait... ARE YOU IN MY HOUSE?


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