Home | Community | Message Board



Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

Jump to first unread post. Pages: 1
OfflineJesus_v2
newbie
Registered: 07/18/03
Posts: 45
Last seen: 12 years, 6 months
PF TEK question about Sterilization
    #1729309 - 07/19/03 03:17 AM (13 years, 7 months ago)

Does Boiling for Sterilization REALLY work, or should I invest in getting a pressure cooker, this is my first time attempting this, I want to make sure I do it right, The Simple Cubensis Growing Tek states that
Quote:

This method works to some degree, and I have heard of people having a 100% success rate using it. More common, however, is that some jars will get contaminated with bacteria, since many bacteria can withstand normal boiling temperatures. I have also heard of people getting 100% contamination using this method.



! So if someone couild perhaps share their own experiences and maybe help me out that would be much appreciated!!

Thanks alot! :mushroom2: 

Is "fractional sterilization" or tyndallization, the method I may need?


Edited by Jesus_v2 (07/19/03 03:19 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,122
Loc: my room
Last seen: 1 month, 13 days
Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1729323 - 07/19/03 03:26 AM (13 years, 7 months ago)

Boiling for 90 minutes usually works, if it doesn?t, look into the other methods.
Or you could inverst in a PC right away, then you can also do whole grains.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAudi0
have ur cake andbeat it 2
Female User Gallery

Registered: 06/27/02
Posts: 10,334
Loc: Dirty South !
Last seen: 5 years, 7 months
Re: PF TEK question about Sterilization [Re: Anno]
    #1729349 - 07/19/03 03:37 AM (13 years, 7 months ago)

im not saying this is 100% contam proof, but i never had one yet.

boil on high for an hour. let cool over night. boil again for an hour and a half the next day.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJesus_v2
newbie
Registered: 07/18/03
Posts: 45
Last seen: 12 years, 6 months
Re: PF TEK question about Sterilization [Re: Audi0]
    #1729356 - 07/19/03 03:43 AM (13 years, 7 months ago)

Thanks d00d's!

Im going to try Audi0's meth cuz its basically the same thing as "fractional sterilization" or tyndallization, so either i will do it just as he states, or maybe add the third boil as in "fractional sterilization". Thanks again though dude

Also whats the best first timer spores to use?
I was thinking of going for Mexican Cube, but I want something cool and pretty, and trippy. Any advice, hints, or tips on that would be greatly appreciated as well!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinephotovorix
retard
Registered: 03/31/03
Posts: 211
Loc: US
Last seen: 12 years, 1 month
Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1729399 - 07/19/03 04:13 AM (13 years, 7 months ago)

HEh.....  does it really work. YES or no one would use it :wink:  I steam for 60 minutes, it works fine, all contams I've had were from poor after sterilization habbits (i.e. needle touching counter, etc)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAudi0
have ur cake andbeat it 2
Female User Gallery

Registered: 06/27/02
Posts: 10,334
Loc: Dirty South !
Last seen: 5 years, 7 months
Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1729404 - 07/19/03 04:17 AM (13 years, 7 months ago)

i like equador. and yeah make sure you sterilize the needle before inooculating and all that good stuff. best of luck.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefactory81
enthusiast

Folding@home Statistics
Registered: 08/22/02
Posts: 314
Loc: Ibiza, Spain
Last seen: 6 years, 11 months
Re: PF TEK question about Sterilization [Re: Audi0]
    #1729749 - 07/19/03 08:57 AM (13 years, 7 months ago)

PF Tek is fine with no PC, given you practice and learn the steralization techniques taught on the forums (lysol, disinefectant wipes, flowhood, tyvek / polyfill, bleach water, verm layer on top, etc), you shouldn't have to big of a issue with contams, and if you do, you shold be able to track it down before you move foward in your mycology learning experience. For grains you need to do pressure cooker, people say it is inevitable you will not be able to get contams to die at 212 F, Pressure cookers heat @ 12 or 15 PSI I forgot, around 250 degrees. So at one of them 2 PSI, is where it will obliterate any contams.
Oh ya, dont forget to clean your needle after every 2 jars.....


Edited by factory81 (07/19/03 08:58 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemikey
Stranger

Registered: 06/04/03
Posts: 548
Re: PF TEK question about Sterilization [Re: factory81]
    #1729780 - 07/19/03 09:53 AM (13 years, 7 months ago)

yeah i heard that works very good


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleTxTec
Texas home andheart breaker...

Registered: 12/19/02
Posts: 1,328
Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1730249 - 07/19/03 04:17 PM (13 years, 7 months ago)

Quote:



Im going to try Audi0's meth
I was thinking of going for Mexican Cube, but I want something cool and pretty, and trippy.


I tried AudiO`s meth once and i couldnt sleep for days... :smirk:


--------------------
I felt a warm warm breeze that melted metal and steele


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblegeorge castanzaM
Lord Of The Idiots!
 User Gallery Arcade Champion: BurgerTime, Space Invaders
Registered: 10/21/02
Posts: 7,291
Trusted Cultivator
Re: PF TEK question about Sterilization [Re: TxTec]
    #1730308 - 07/19/03 04:45 PM (13 years, 7 months ago)

u can also oven bake your brf/verm although the moister content is a little trickier that way

says kramer


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Two PF Tek Questions Enneagram 2,685 14 03/17/13 01:47 PM
by Acero
* PF Tek question forevadazin 1,327 11 11/04/02 05:06 PM
by Knives_Out
* A quick PF-Tek question.. darkbuster 609 4 07/05/06 03:13 AM
by creamcorn
* PF tek question phunkjunky311 1,001 14 07/23/04 01:34 PM
by Seppuko
* pf tek questions sinsemillia 763 8 04/29/04 02:35 AM
by Anno
* Some more "PF Tek" questions... ShroomNoob03 2,583 4 01/18/12 05:17 PM
by Dominar
* Simple PF-Tek question mycoresearch 571 3 06/28/04 06:15 PM
by mycoresearch
* Pf-tek question FuNgUsMaN420 1,243 17 12/18/02 08:59 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
732 topic views. 36 members, 88 guests and 20 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
The Spore Depot
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.069 seconds spending 0.005 seconds on 14 queries.