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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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OfflineJesus_v2
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Registered: 07/18/03
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PF TEK question about Sterilization
    #1729309 - 07/19/03 03:17 AM (14 years, 1 month ago)

Does Boiling for Sterilization REALLY work, or should I invest in getting a pressure cooker, this is my first time attempting this, I want to make sure I do it right, The Simple Cubensis Growing Tek states that
Quote:

This method works to some degree, and I have heard of people having a 100% success rate using it. More common, however, is that some jars will get contaminated with bacteria, since many bacteria can withstand normal boiling temperatures. I have also heard of people getting 100% contamination using this method.



! So if someone couild perhaps share their own experiences and maybe help me out that would be much appreciated!!

Thanks alot! :mushroom2: 

Is "fractional sterilization" or tyndallization, the method I may need?


Edited by Jesus_v2 (07/19/03 03:19 AM)


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OfflineAnnoA
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Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1729323 - 07/19/03 03:26 AM (14 years, 1 month ago)

Boiling for 90 minutes usually works, if it doesn?t, look into the other methods.
Or you could inverst in a PC right away, then you can also do whole grains.


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OfflineAudi0
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Re: PF TEK question about Sterilization [Re: Anno]
    #1729349 - 07/19/03 03:37 AM (14 years, 1 month ago)

im not saying this is 100% contam proof, but i never had one yet.

boil on high for an hour. let cool over night. boil again for an hour and a half the next day.


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OfflineJesus_v2
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Re: PF TEK question about Sterilization [Re: Audi0]
    #1729356 - 07/19/03 03:43 AM (14 years, 1 month ago)

Thanks d00d's!

Im going to try Audi0's meth cuz its basically the same thing as "fractional sterilization" or tyndallization, so either i will do it just as he states, or maybe add the third boil as in "fractional sterilization". Thanks again though dude

Also whats the best first timer spores to use?
I was thinking of going for Mexican Cube, but I want something cool and pretty, and trippy. Any advice, hints, or tips on that would be greatly appreciated as well!


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Offlinephotovorix
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Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1729399 - 07/19/03 04:13 AM (14 years, 1 month ago)

HEh.....  does it really work. YES or no one would use it :wink:  I steam for 60 minutes, it works fine, all contams I've had were from poor after sterilization habbits (i.e. needle touching counter, etc)


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OfflineAudi0
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Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1729404 - 07/19/03 04:17 AM (14 years, 1 month ago)

i like equador. and yeah make sure you sterilize the needle before inooculating and all that good stuff. best of luck.


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Offlinefactory81
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Re: PF TEK question about Sterilization [Re: Audi0]
    #1729749 - 07/19/03 08:57 AM (14 years, 1 month ago)

PF Tek is fine with no PC, given you practice and learn the steralization techniques taught on the forums (lysol, disinefectant wipes, flowhood, tyvek / polyfill, bleach water, verm layer on top, etc), you shouldn't have to big of a issue with contams, and if you do, you shold be able to track it down before you move foward in your mycology learning experience. For grains you need to do pressure cooker, people say it is inevitable you will not be able to get contams to die at 212 F, Pressure cookers heat @ 12 or 15 PSI I forgot, around 250 degrees. So at one of them 2 PSI, is where it will obliterate any contams.
Oh ya, dont forget to clean your needle after every 2 jars.....


Edited by factory81 (07/19/03 08:58 AM)


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Invisiblemikey
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Re: PF TEK question about Sterilization [Re: factory81]
    #1729780 - 07/19/03 09:53 AM (14 years, 1 month ago)

yeah i heard that works very good


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InvisibleTxTec
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Re: PF TEK question about Sterilization [Re: Jesus_v2]
    #1730249 - 07/19/03 04:17 PM (14 years, 1 month ago)

Quote:



Im going to try Audi0's meth
I was thinking of going for Mexican Cube, but I want something cool and pretty, and trippy.


I tried AudiO`s meth once and i couldnt sleep for days... :smirk:


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I felt a warm warm breeze that melted metal and steele


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Invisiblegeorge castanzaM
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Re: PF TEK question about Sterilization [Re: TxTec]
    #1730308 - 07/19/03 04:45 PM (14 years, 1 month ago)

u can also oven bake your brf/verm although the moister content is a little trickier that way

says kramer


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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