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trip352
the not so new guy



Registered: 03/23/11
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Beware of bad LC??
#17284897 - 11/26/12 02:19 PM (12 years, 1 month ago) |
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I think the LC was bad with bacteria and I didn't really "test" it before making the spawn.... What you guys think
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Beware of bad LC?? [Re: trip352]
#17284903 - 11/26/12 02:20 PM (12 years, 1 month ago) |
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Maybe I'm missing something but those look ok  Can you get a close up of the sections that are suspect?
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trip352
the not so new guy



Registered: 03/23/11
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Well the cakes have been doing that on and off ever since I stared useing GLC from MS
Almost a yellowing starting from the top... Mycillia his it but stops dead in its tracks.... And it goes kinda deep it seems
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trip352
the not so new guy



Registered: 03/23/11
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Re: Beware of bad LC?? [Re: trip352]
#17284999 - 11/26/12 02:39 PM (12 years, 1 month ago) |
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That's a bit closer... There's just not any rizo. Development ... I haven't done the smell test yet though
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
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Re: Beware of bad LC?? [Re: trip352]
#17285020 - 11/26/12 02:45 PM (12 years, 1 month ago) |
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Try & break one up & smell it. Just because you don't see rhizomorphic growth, doesn't mean they're contaminated. If those jars looked 100%, then the bottoms started "decolonizing" it's a contam, for sure. Some sort of Bacteria & or mold.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Quote:
If those jars looked 100%, then the bottoms started "decolonizing" it's a contam, for sure.
I've never seen that happen personally. What are the usual causes? Bacteria or mould? Can both contaminants cause this to happen?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
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Initially, I thought it was bacteria, but I let a few jars sit longer & pink/lipstick mold popped up.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Interesting. I've seen pictures of this phenomenon before, but never first hand. I was just curious, thank you.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
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Yea, I had it in around 20 jars...bad GLC to LC.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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I just g2g now. LC's gave me too much of a head ache. 20 jars here to bacteria, 40 jars here to bacteria. These were AFTER tests as well.
Oh well, everyone will gravitate towards what they prefer and what works for them.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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I haven't made an LC or GLC in over a year. G2Gs =
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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What about the yellowing in the cake?
And thanks TL i just finished your Glove box tek... So now that I'm not on open air inoculations ill be trying agar work
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twistedty
Forcefully Retired



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Quote:
TranscendingLife said: I haven't made an LC or GLC in over a year. G2Gs = 
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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Re: Beware of bad LC?? [Re: twistedty]
#17285918 - 11/26/12 05:17 PM (12 years, 1 month ago) |
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With a g2g... How long is full colonization estimated? Is a month way to long?
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twistedty
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Re: Beware of bad LC?? [Re: trip352]
#17285962 - 11/26/12 05:22 PM (12 years, 1 month ago) |
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Quote:
trip352 said: With a g2g... How long is full colonization estimated? Is a month way to long?
yes g2g boasts quickest colonization IME. a month is way to long IMO
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PussyFart
Retired Cultivation Extrodinaire



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Re: Beware of bad LC?? [Re: twistedty]
#17285982 - 11/26/12 05:23 PM (12 years, 1 month ago) |
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I can turn one fully colonized quart into 100 in a little more than 3 weeks time...maybe 4, depending on the culture.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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TranscendingLife
I Don't Need a Life to Live



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Posts: 21,627
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Re: Beware of bad LC?? [Re: trip352]
#17287539 - 11/26/12 10:09 PM (12 years, 1 month ago) |
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Quote:
trip352 said: With a g2g... How long is full colonization estimated? Is a month way to long?
2 weeks is my average for a non-isolate culture. It also depends on how many jars you G2G to. If you're doing 1 quart to 5, they'll colonize in 1/2 the time of 1 to 10. Normally, I do 1 jar to 8. 7 jars for spawning to bulk & 1 jar to G2G. Sometimes, it's 6 then 2.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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It takes my g2g receiving quart jars 14 days give or take to colonize.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,285
Loc: N38.93829W119.98108
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Those jars do look like bad. You will probably be able to fruit them but expect low yeilds.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987
Teh=The
I need to proofread
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trip352
the not so new guy



Registered: 03/23/11
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Last seen: 8 years, 9 months
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What could be a cause of slow colonization? Possibly I dried the grains out to much before pressure cooking?? The growth looks great though... Lol
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TranscendingLife
I Don't Need a Life to Live



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Re: Beware of bad LC?? [Re: trip352]
#17288137 - 11/27/12 12:03 AM (12 years, 1 month ago) |
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Quote:
trip352 said: What could be a cause of slow colonization? Possibly I dried the grains out to much before pressure cooking??
Moisture content, GE filters, etc. The idea is to be a scientist. Start messing with variables & use what works.
Quote:
trip352 said: The growth looks great though... Lol
So does mold to the untrained eye. The way your jars look 100% colonized on the top & 80% on the bottom are true signs of contamination. Let those jars sit for 2 more days & see how they look.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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Lol I meant in my other jars that I used a fresh spore syring on ....
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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Re: Beware of bad LC?? [Re: trip352]
#17322309 - 12/02/12 06:18 PM (12 years, 1 month ago) |
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Been a few days and I see zero change in the jars... Should I spawn and hope orrrrrr? Also if you spawn to 50/50 horse/coir... Would it be best to pasteurize even if working with a 1:1 spawn ratio?
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PussyFart
Retired Cultivation Extrodinaire



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Re: Beware of bad LC?? [Re: trip352]
#17322360 - 12/02/12 06:27 PM (12 years, 1 month ago) |
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Quote:
trip352 said: Also if you spawn to 50/50 horse/coir... Would it be best to pasteurize even if working with a 1:1 spawn ratio?
Bulk substrates ALWAYS get pasteurized, the spawn ratio has noting to do with it. For manure you need to do proper pasteurization.
Also, adding some vermiculite in your substrate mix might help a little with moisture retention, not to mention it has nutrients the mycellium crave as well.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (12/02/12 06:28 PM)
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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Re: Beware of bad LC?? [Re: PussyFart]
#17323098 - 12/02/12 08:25 PM (12 years, 1 month ago) |
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Vermiculite has nutes??? Whaaaa?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Re: Beware of bad LC?? [Re: trip352]
#17323185 - 12/02/12 08:39 PM (12 years, 1 month ago) |
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Quote:
trip352 said: Vermiculite has nutes??? Whaaaa?
Yup...
Quote:
RogerRabbit said: What the hell is this crap I read above, saying there's no nutrients in vermiculite?
Why do you suppose the brf tek is 2/3 verm? Because fungi has need of minerals, that's why. I want to rip someone's ears off when I read verm has no 'nutes'. There's lots of material that will hold as much water as vermiculite, such as peatmoss, sand, coir, cardboard, shredded newspapers, and about ten thousand others, but verm is what we use because it has many of the minerals that mushrooms want, as does gypsum.
However, brf cakes are already 2/3 verm so find another nutrient source if you want to move on from there. RR
http://www.shroomery.org/forums/showflat.php/Number/14107914#14107914
Quote:
RogerRabbit said: Actually, verm does have nutrients for contaminants as well as mushroom mycelium. Fungi digest minerals. RR
http://www.shroomery.org/forums/showflat.php/Number/13665989#13665989
Quote:
TranscendingLife said: Also, coir has nutrients, so does verm (trace minerals), which is why they're not proper casing materials by themselves.
http://www.shroomery.org/forums/showflat.php/Number/17275917#17275917
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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Re: Beware of bad LC?? [Re: PussyFart]
#17323241 - 12/02/12 08:48 PM (12 years, 1 month ago) |
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Lol true shit I remember the minerals thing but I dunno guess I'm just thinking bout these jars to much....
Would you toss it or try to make a tray?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Beware of bad LC?? [Re: trip352]
#17323248 - 12/02/12 08:49 PM (12 years, 1 month ago) |
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Quote:
trip352 said:

All of those jars are contaminated. Look at the bottoms vs the tops.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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That's what I was saying but some one told me to wait a few more days and see
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Beware of bad LC?? [Re: trip352]
#17323339 - 12/02/12 09:02 PM (12 years, 1 month ago) |
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Yea. & whoever told you to spawn those is wrong.
I remember saying wait a few more days & see. & now, it's clear. Those are definitely contaminated. Ditch them before they start to spore.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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Rahz
Alive Again



Registered: 11/10/05
Posts: 10,315
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Re: Beware of bad LC?? [Re: trip352]
#17323351 - 12/02/12 09:05 PM (12 years, 1 month ago) |
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TL is right. Those uncolonized layers at the bottom are unusual and highly suspect. I wouldn't spawn them, not inside at least.
-------------------- rahz
comfort pleasure power love truth awareness peace I am I feel I do I love I speak I see I know
"If you knew how quickly people forget the dead... you would stop living to impress people." — Christopher Walken
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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Re: Beware of bad LC?? [Re: Rahz]
#17323423 - 12/02/12 09:15 PM (12 years, 1 month ago) |
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Lol dump in progress... So here's another question cause its from the same LC and the pf cakes didn't all contaminate....least not visually... I've seen a couple start pinning and fruit body formation with out full colonization.... That's a first for me... I mean I know it could be from contamination but the pf cakes look fine to me
Also... Is there any known way to make a GH for cakes... Every time I ask or search I get told GHs need bulk but I've heard whispers that Ultra sonics are needed for cakes and that's why people swear by them
Edited by trip352 (12/02/12 09:22 PM)
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Rahz
Alive Again



Registered: 11/10/05
Posts: 10,315
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Re: Beware of bad LC?? [Re: trip352]
#17323552 - 12/02/12 09:33 PM (12 years, 1 month ago) |
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Hard to tell. If it's not from the LC then it's from somewhere else, it's just a matter of going back over every step in the process. If everything else looks good then you start to suspect the culture. If the cakes experienced similar problems that could lean the odds toward a bad culture.
-------------------- rahz
comfort pleasure power love truth awareness peace I am I feel I do I love I speak I see I know
"If you knew how quickly people forget the dead... you would stop living to impress people." — Christopher Walken
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 8 years, 9 months
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Re: Beware of bad LC?? [Re: Rahz]
#17323868 - 12/02/12 10:36 PM (12 years, 1 month ago) |
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Well it was all GLC.... Possibly the syringe didn't stay steril? I did the boil water in and out a few times tek
Edited by trip352 (12/02/12 10:37 PM)
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Beware of bad LC?? [Re: trip352]
#17324075 - 12/02/12 11:24 PM (12 years, 1 month ago) |
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Possibly the syringe, or it happened when aspirating the LC from the jar...shit, with LC, there's no telling when the contam entered.
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trip352
the not so new guy



Registered: 03/23/11
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I thought about that... How dose the pressure of over 120cc +/- effect the pressure with a Filter disk? 3/8th hole
Edited by trip352 (12/03/12 12:25 AM)
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