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InvisibleTranscendingLife
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Re: Beware of bad LC?? [Re: trip352]
    #17288137 - 11/27/12 12:03 AM (12 years, 1 month ago)

Quote:

trip352 said:
What could be a cause of slow colonization? Possibly I dried the grains out to much before pressure cooking??




Moisture content, GE filters, etc.
The idea is to be a scientist.
Start messing with variables & use what works.

Quote:

trip352 said:
The growth looks great though... Lol




So does mold to the untrained eye.
The way your jars look 100% colonized on the top & 80% on the bottom are true signs of contamination.
Let those jars sit for 2 more days & see how they look.


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Offlinetrip352
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Re: Beware of bad LC?? [Re: TranscendingLife]
    #17288437 - 11/27/12 02:01 AM (12 years, 1 month ago)

Lol I meant in my other jars that I used a fresh spore syring on ....

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Offlinetrip352
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Re: Beware of bad LC?? [Re: trip352]
    #17322309 - 12/02/12 06:18 PM (12 years, 1 month ago)




Been a few days and I see zero change in the jars... Should I spawn and hope orrrrrr?  Also if you spawn to 50/50 horse/coir... Would it be best to pasteurize even if working with a 1:1 spawn ratio?

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OfflinePussyFart
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Re: Beware of bad LC?? [Re: trip352]
    #17322360 - 12/02/12 06:27 PM (12 years, 1 month ago)

Quote:

trip352 said:
Also if you spawn to 50/50 horse/coir... Would it be best to pasteurize even if working with a 1:1 spawn ratio?



Bulk substrates ALWAYS get pasteurized, the spawn ratio has noting to do with it. For manure you need to do proper pasteurization.

Also, adding some vermiculite in your substrate mix might help a little with moisture retention, not to mention it has nutrients the mycellium crave as well.

Edited by PussyFart (12/02/12 06:28 PM)

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Offlinetrip352
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Re: Beware of bad LC?? [Re: PussyFart]
    #17323098 - 12/02/12 08:25 PM (12 years, 1 month ago)

Vermiculite has nutes??? Whaaaa?

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OfflinePussyFart
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Re: Beware of bad LC?? [Re: trip352]
    #17323185 - 12/02/12 08:39 PM (12 years, 1 month ago)

Quote:

trip352 said:
Vermiculite has nutes??? Whaaaa?





Yup...


Quote:

RogerRabbit said:
What the hell is this crap I read above, saying there's no nutrients in vermiculite?

Why do you suppose the brf tek is 2/3 verm?  Because fungi has need of minerals, that's why.  I want to rip someone's ears off when I read verm has no 'nutes'.  There's lots of material that will hold as much water as vermiculite, such as peatmoss, sand, coir, cardboard, shredded newspapers, and about ten thousand others,  but verm is what we use because it has many of the minerals that mushrooms want, as does gypsum.

However, brf cakes are already 2/3 verm so find another nutrient source if you want to move on from there. 
RR



http://www.shroomery.org/forums/showflat.php/Number/14107914#14107914

Quote:

RogerRabbit said:
Actually, verm does have nutrients for contaminants as well as mushroom mycelium.  Fungi digest minerals.
RR



http://www.shroomery.org/forums/showflat.php/Number/13665989#13665989

Quote:

TranscendingLife said:
Also, coir has nutrients, so does verm (trace minerals), which is why they're not proper casing materials by themselves.




http://www.shroomery.org/forums/showflat.php/Number/17275917#17275917

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Offlinetrip352
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Re: Beware of bad LC?? [Re: PussyFart]
    #17323241 - 12/02/12 08:48 PM (12 years, 1 month ago)

Lol true shit I remember the minerals thing but I dunno guess I'm just thinking bout these jars to much....

Would you toss it or try to make a tray?

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InvisibleTranscendingLife
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Re: Beware of bad LC?? [Re: trip352]
    #17323248 - 12/02/12 08:49 PM (12 years, 1 month ago)

Quote:

trip352 said:






All of those jars are contaminated.
Look at the bottoms vs the tops.


--------------------
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"One must accept the probability of failure to experience the elation of success." - TranscendingLife
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"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
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Re: Beware of bad LC?? [Re: TranscendingLife]
    #17323307 - 12/02/12 08:58 PM (12 years, 1 month ago)

That's what I was saying but some one told me to wait a few more days and see

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Re: Beware of bad LC?? [Re: trip352]
    #17323339 - 12/02/12 09:02 PM (12 years, 1 month ago)

Yea. & whoever told you to spawn those is wrong.

I remember saying wait a few more days & see.
& now, it's clear. Those are definitely contaminated.
Ditch them before they start to spore.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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InvisibleRahz
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Re: Beware of bad LC?? [Re: trip352]
    #17323351 - 12/02/12 09:05 PM (12 years, 1 month ago)

TL is right. Those uncolonized layers at the bottom are unusual and highly suspect. I wouldn't spawn them, not inside at least.


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Re: Beware of bad LC?? [Re: Rahz]
    #17323423 - 12/02/12 09:15 PM (12 years, 1 month ago)

Lol dump in progress... So here's another question cause its from the same LC and the pf cakes didn't all contaminate....least not visually... I've seen a couple start pinning and fruit body formation with out full colonization.... That's a first for me... I mean I know it could be from contamination but the pf cakes look fine to me

Also... Is there any known way to make a GH for cakes... Every time I ask or search I get told GHs need bulk but I've heard whispers that Ultra sonics are needed for cakes and that's why people swear by them

Edited by trip352 (12/02/12 09:22 PM)

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InvisibleRahz
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Re: Beware of bad LC?? [Re: trip352]
    #17323552 - 12/02/12 09:33 PM (12 years, 1 month ago)

Hard to tell. If it's not from the LC then it's from somewhere else, it's just a matter of going back over every step in the process. If everything else looks good then you start to suspect the culture. If the cakes experienced similar problems that could lean the odds toward a bad culture.


--------------------
rahz

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Re: Beware of bad LC?? [Re: Rahz]
    #17323868 - 12/02/12 10:36 PM (12 years, 1 month ago)

Well it was all GLC.... Possibly the syringe didn't stay steril? I did the boil water in and out a few times tek

Edited by trip352 (12/02/12 10:37 PM)

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InvisibleFrankHorrigan
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Re: Beware of bad LC?? [Re: trip352]
    #17324075 - 12/02/12 11:24 PM (12 years, 1 month ago)

Possibly the syringe, or it happened when aspirating the LC from the jar...shit, with LC, there's no telling when the contam entered.


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Re: Beware of bad LC?? [Re: FrankHorrigan]
    #17324359 - 12/03/12 12:24 AM (12 years, 1 month ago)

I thought about that... How dose the pressure of over 120cc +/- effect the pressure with a Filter disk? 3/8th hole

Edited by trip352 (12/03/12 12:25 AM)

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