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Offline11111
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Cubensis Mono Culture? or close? (pics)
    #17277269 - 11/25/12 03:21 AM (11 years, 5 months ago)

Wasabi
Im in the search for an awesome "mono" culture. I started with MS > Rye grain > 1 grain on agar. Pictured is the third transfer for all plates. I know that I must fruit potential "mono's" to see which is best, but i have run into 3 different growth patterns.

First, I have about 5 of 20 plates which are showing alot of Rizo growth still. But to me, looks like healthy material worth transferring one more time...


Then I have almost 10 of 20 plates which are super boring to me. In that they are kinda weak in growth, cottony (not mold like but weak mycelium), BUT they are perfect circles. I was gunna disregard all dishes that looked like this, but some one save them if it is "mono" potential.


Then these are the ones that I think are "mono" cultures. The reason i think this is because they have grown pretty circular, even creating rings of growth which to me looks bad ass! but also in a sense that they could be strong producers? Can some one reassure me? I am doing one more transfer with these tomorrow to new dishes and then hopefully test fruit after that... 
(no, not a pube and yes, pictures will show how much more cleanly one must be. ruined the pic for me!)

hope to show the results of this first ever run with agar soon! super awesome how much knowledge one can gain on this site. (btw im not just posting pics, im a noob and want to make sure i am on the right path towards strong species)  :bouncysmoke:

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Offlinecloudpersona
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Re: Cubensis Mono Culture? or close? (pics) [Re: 11111]
    #17279427 - 11/25/12 02:52 PM (11 years, 5 months ago)

Looks mono to me.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
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Invisibleuncle_rico
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Re: Cubensis Mono Culture? or close? (pics) [Re: 11111]
    #17279588 - 11/25/12 03:25 PM (11 years, 5 months ago)

Quote:

11111 said:






I'd take the very 'leading edge' of the rhizo sector at 11:30.  just a little bit.

See if that will grow into a perfect rhizo circle.

Looks nice.  good luck  MAN


--------------------

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Offline11111
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Re: Cubensis Mono Culture? or close? (pics) [Re: uncle_rico]
    #17279678 - 11/25/12 03:43 PM (11 years, 5 months ago)

Quote:

cloudpersona said:
Looks mono to me.



Quote:

uncle_rico said:
Quote:

11111 said:






I'd take the very 'leading edge' of the rhizo sector at 11:30.  just a little bit.

See if that will grow into a perfect rhizo circle.

Looks nice.  good luck  MAN




right on yall! ill get these to grain soon. No one commented about the weak looking circular mycelium. worth even fruiting out to test or just leave um? Thanks!

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Offlinecloudpersona
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Re: Cubensis Mono Culture? or close? (pics) [Re: 11111]
    #17279721 - 11/25/12 03:52 PM (11 years, 5 months ago)

Rhizo generally means stronger, and more aggressive myc. The weak looking myc probably is weak.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
– Terence McKenna
“If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.”
-Terence McKenna

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Offlinejacobensis
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Re: Cubensis Mono Culture? or close? (pics) [Re: cloudpersona]
    #17280489 - 11/25/12 05:41 PM (11 years, 5 months ago)

Ive done this befor myself and can tell you that the weaker looking plates you have can develope into strong monos. id take the leading strongest edge of each of those and transfer- you`ve came this far. with the strong mono`s you have, you`ll definitely get a good isolated type/substrain from them, of course you`re going to eventually fruit those, thats where you want to make plenty of prints- if they are strong spore producers, and have good potency. those are the next factors you have to work out. its exciting to see someone doing agar work! thumbs up to you my friend!!!!


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Invisibleuncle_rico
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Re: Cubensis Mono Culture? or close? (pics) [Re: 11111]
    #17280788 - 11/25/12 06:23 PM (11 years, 5 months ago)

http://www.shroomery.org/forums/thumbs/12-42/060112032-thumb_attachment.jpg

Some of the best performing cultures I have worked with (ripped through grain spawn and bulk substrates and fruited like mad) have not been totally rhizomorphic.

The above image is from Professor P's 'no pour agar tek' .. it is PE .. and I'll bet it is a big performer .. and it looks similar to your mono cultures.

So ... expand them on grain and do some tubs and check them out !

good luck, my friend.


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Offline11111
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Re: Cubensis Mono Culture? or close? (pics) [Re: uncle_rico]
    #17281324 - 11/25/12 08:00 PM (11 years, 5 months ago)

Quote:

jacobensis said:
Ive done this befor myself and can tell you that the weaker looking plates you have can develope into strong monos. id take the leading strongest edge of each of those and transfer- you`ve came this far. with the strong mono`s you have, you`ll definitely get a good isolated type/substrain from them, of course you`re going to eventually fruit those, thats where you want to make plenty of prints- if they are strong spore producers, and have good potency. those are the next factors you have to work out. its exciting to see someone doing agar work! thumbs up to you my friend!!!!




Thanks! and thank you for the tips! much appreciated, and hope to show the results soon!
Quote:

uncle_rico said:
http://www.shroomery.org/forums/thumbs/12-42/060112032-thumb_attachment.jpg

Some of the best performing cultures I have worked with (ripped through grain spawn and bulk substrates and fruited like mad) have not been totally rhizomorphic.

The above image is from Professor P's 'no pour agar tek' .. it is PE .. and I'll bet it is a big performer .. and it looks similar to your mono cultures.

So ... expand them on grain and do some tubs and check them out !

good luck, my friend.



Nice! thanks for the input, i needed it. I will save a few of the weaker ones for transfers then. I think PE is my next species for sure...

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OfflinePrimalSoup
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Re: Cubensis Mono Culture? or close? (pics) [Re: 11111]
    #17312171 - 11/30/12 10:05 PM (11 years, 5 months ago)

First row of plates are still sectoring, keep on isolating.  I'm with uncle_rico on this.

What you've got in the rest of the pics is tomentose - yeah, it's all circular and so on but if you want rhyzo (most people do as it seems to colonize and fruit better) go with the a-list above.  I've fruited cottony myc when I couldn't get anything else from certain spores, though. :thumbup:

Here's a pic of what's closer to a monoculture for me:

:peace:PS


--------------------

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Offline11111
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Re: Cubensis Mono Culture? or close? (pics) [Re: PrimalSoup]
    #17312718 - 12/01/12 12:38 AM (11 years, 5 months ago)

Quote:

PrimalSoup said:
First row of plates are still sectoring, keep on isolating.  I'm with uncle_rico on this.

What you've got in the rest of the pics is tomentose - yeah, it's all circular and so on but if you want rhyzo (most people do as it seems to colonize and fruit better) go with the a-list above.  I've fruited cottony myc when I couldn't get anything else from certain spores, though. :thumbup:

Here's a pic of what's closer to a monoculture for me:

:peace:PS




hell yeah thanks for the reply and that def looks legit! ill post a couple pics in a few days of those transfers i did that day. hopefully they will look like that...

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