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OfflineTheUnknownPoet
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Malt with Yeast
    #17274822 - 11/24/12 05:31 PM (11 years, 5 months ago)

The only place I can find light malt extract is in beer brew kits.
However, these all have yest mixed in them.

Could I still make an agar solution from this?

I would expect to find light malt extract and agar agara powered/flakes at a bulk barn, but no such luck. Nobody knows what this stuff even is here.

Will malt extract from a beer kit work for an agar mix?

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OfflineJeff
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Re: Malt with Yeast [Re: TheUnknownPoet]
    #17274838 - 11/24/12 05:36 PM (11 years, 5 months ago)



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Myco-tek

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OfflineTmethylM
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Re: Malt with Yeast [Re: Jeff]
    #17274851 - 11/24/12 05:39 PM (11 years, 5 months ago)

Yeast is sometimes used in liquid cultures and agar, it's a nutritional source for mycelium, but you have to be sure that the yeast is killed in the sterilization process or it will contaminate very rapidly.


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¯\_(ツ)_/¯

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OfflineJeff
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Re: Malt with Yeast [Re: Tmethyl]
    #17274944 - 11/24/12 05:58 PM (11 years, 5 months ago)

It is but it for the most part is deactivated.yeast
I would not recommend messing around with live yeast unless it had been thoroughly pc'd.


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Myco-tek

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OfflineOICU812
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Re: Malt with Yeast [Re: Jeff]
    #17275656 - 11/24/12 08:37 PM (11 years, 5 months ago)

Here is an online source for Extra Light Dried Malt Extract without yeast at an economical price.


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"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson

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OfflineTheUnknownPoet
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Re: Malt with Yeast [Re: Jeff]
    #17318834 - 12/02/12 03:10 AM (11 years, 5 months ago)

Quote:

Jeff said:
It is but it for the most part is deactivated.yeast
I would not recommend messing around with live yeast unless it had been thoroughly pc'd.




I think I'll just follow a normal tek only with the yeast mixed in and agar agar, then PC it.

Will the dead yest actually help my mycelim grow?

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OfflineTmethylM
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Re: Malt with Yeast [Re: TheUnknownPoet]
    #17318920 - 12/02/12 03:51 AM (11 years, 5 months ago)

Quote:

TheUnknownPoet said:
Quote:

Jeff said:
It is but it for the most part is deactivated.yeast
I would not recommend messing around with live yeast unless it had been thoroughly pc'd.




I think I'll just follow a normal tek only with the yeast mixed in and agar agar, then PC it.

Will the dead yest actually help my mycelim grow?



Yes dead yiest is nutrtonal


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¯\_(ツ)_/¯

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InvisibleZeet
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Re: Malt with Yeast [Re: Tmethyl]
    #17320181 - 12/02/12 11:42 AM (11 years, 5 months ago)

Yeast and agar agar is sufficient for agar plate work?

Last time I made agar I used honey, dried potato flakes and agar agar.
Mycelium was very slow growing on it..

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OfflineJeff
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Re: Malt with Yeast [Re: Zeet]
    #17320690 - 12/02/12 01:21 PM (11 years, 5 months ago)

No it is mixed in at usually 2 grams per liter in a PDA or MEA or for that matter any other recipe.  It is just a bit of added nutrition.


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Myco-tek

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InvisibleZeet
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Re: Malt with Yeast [Re: Jeff]
    #17321142 - 12/02/12 02:37 PM (11 years, 5 months ago)

Ah ok.. Shame! I got excited then!
Is Barley malt extract ok for agar work?

Might get another batch of agar plates on the go.. It was very fun and I definitely felt like a top scientist!


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OfflineJeff
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Re: Malt with Yeast [Re: Zeet]
    #17321165 - 12/02/12 02:41 PM (11 years, 5 months ago)

Yup, that is generally the type of malt that is used.


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Myco-tek

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OfflineOICU812
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Re: Malt with Yeast [Re: Zeet]
    #17321930 - 12/02/12 04:57 PM (11 years, 5 months ago)

Quote:

Zeet said:
Ah ok.. Shame! I got excited then!
Is Barley malt extract ok for agar work?

Might get another batch of agar plates on the go.. It was very fun and I definitely felt like a top scientist!






Here is a link to FastFred's Media Cookbook that has over 50 media formulas.  Hope this helps a little!


--------------------
--------------
"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson

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