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OfflineBertnewbblaster
Fungi Fanatic

Registered: 10/25/12
Posts: 8
Last seen: 12 years, 16 days
Second times a charm!
    #17270147 - 11/23/12 05:45 PM (12 years, 1 month ago)

I started a grow of Cambodian Cubes about 4 weeks ago that failed miserably to contamination. Failing so bad the first time really makes you respect the sterilization aspect of the process. Today a friend and I started our second attempt. My outlook is positive this time as we didn't cut any corners and I can't think of any weakness in our defense against contaminants. Wish me luck! Hopefully I'll have some Cambodians and Golden Teachers for Shroomsday! :mushroom2:


--------------------
~ Bert

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OfflineKalypto
Psychonaut
Male

Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 11 months
Re: Second times a charm! [Re: Bertnewbblaster]
    #17270160 - 11/23/12 05:48 PM (12 years, 1 month ago)

My first two attmepts failed due to bad inoculate , if all your jars fail its a possibility, one way you can confirm this is to not inoculate a jar and see if it contaminates with your inoculated jar.

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OfflineBertnewbblaster
Fungi Fanatic

Registered: 10/25/12
Posts: 8
Last seen: 12 years, 16 days
Re: Second times a charm! [Re: Kalypto]
    #17273538 - 11/24/12 12:21 PM (12 years, 1 month ago)

My first attempt I used a syringe from a company out of Vancouver but this time I am trying a syringe from that company plus two from another source. We did 28 jars total and they are different styles of 1/2 pint jars to easily tell them apart.

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OfflineKalypto
Psychonaut
Male

Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 11 months
Re: Second times a charm! [Re: Bertnewbblaster]
    #17274602 - 11/24/12 04:32 PM (12 years, 1 month ago)

Kool , i recommend sporeworks <3

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OfflineBertnewbblaster
Fungi Fanatic

Registered: 10/25/12
Posts: 8
Last seen: 12 years, 16 days
Re: Second times a charm! [Re: Kalypto]
    #17311829 - 11/30/12 09:01 PM (12 years, 1 month ago)

Update

7 jars are contaminated
11 have no growth
10 are looking good

The 7 contaminated jars have been removed from the incubator. These jars are from one of the two syringes I received from a company I wasn't sure about because of other reviews on here but decided to try anyway because of the cheap prices. The 10 jars that are showing good mycelium growth are from a separate syringe also purchased from this company. Both were golden teacher cubes.

The 11 with no growth came from the Vancouver company and are Cambodian Cubes. This is a reputable company but the syringe was almost a year old.

I'm glad we used different sources. I am confident in our procedure. we heated the needles between jars so I'm sure the contams are from a bad syringe. This is a learning experience and I can't wait to be able to grab some of my own spore prints so I don't have to take chances with syringes made poorly.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Second times a charm! [Re: Bertnewbblaster]
    #17312246 - 11/30/12 10:17 PM (12 years, 1 month ago)

Quote:

This is a learning experience and I can't wait to be able to grab some of my own spore prints so I don't have to take chances with syringes made poorly.




Use a sponsor and this will be a non-issue.

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OfflineTrippy_Smurf
Sketchy Mother Fucker
Male User Gallery


Folding@home Statistics
Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage Flag
Last seen: 1 month, 10 days
Re: Second times a charm! [Re: Kalypto]
    #17312305 - 11/30/12 10:28 PM (12 years, 1 month ago)

Quote:

Kalypto said:
Kool , i recommend *********** <3




Please don't recommend that sponsors' products be used for illegal activities, and remove their name from that post.  They sell them for microscopy use only.  If you choose to use them illegally, that's all on you.


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