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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 11 months
other ways to rehydrate spores....
    #1726270 - 07/17/03 11:08 PM (14 years, 1 month ago)

Is there some simpler way to accomplish this easy-sounding task?

I have no intention of sending spores anywhere or moving them much more than a couple feet, so would it be possible for me to simply boil some water to steralize it, let cool, add spores, wait a day or however long it takes for them to rehydrate and pour on substrate?


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InvisibleGestaltAssault
Sam Kohui (c)

Registered: 05/25/03
Posts: 420
Loc: Here-->[x]
Re: other ways to rehydrate spores.... [Re: Ravinoff]
    #1726282 - 07/17/03 11:14 PM (14 years, 1 month ago)

pour on substrate? what happened to the syringe??


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Anonymous

Re: other ways to rehydrate spores.... [Re: Ravinoff]
    #1726283 - 07/17/03 11:14 PM (14 years, 1 month ago)

Ummm. no........ read the faqs and focus on a syringe tek.


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 11 months
Re: other ways to rehydrate spores.... [Re: ]
    #1728950 - 07/18/03 11:52 PM (14 years, 1 month ago)

i meant like put the spores in a bowl of steralized water, i have no syringes...

someone please answer, i think pitdawg has no idea what he's talking about


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Offlineshakta
Infidel
Registered: 06/03/03
Posts: 2,633
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Re: other ways to rehydrate spores.... [Re: Ravinoff]
    #1729018 - 07/19/03 12:24 AM (14 years, 1 month ago)

Quote:

Ravinoff said:
i meant like put the spores in a bowl of steralized water, i have no syringes...

someone please answer, i think pitdawg has no idea what he's talking about




Sounds more like you don't to me. I would make a syringe as well.


Edited by shakta (07/19/03 12:24 AM)


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 11 months
Re: other ways to rehydrate spores.... [Re: shakta]
    #1729070 - 07/19/03 12:51 AM (14 years, 1 month ago)

maybe if you had a tiny bit of intelligence, you would realize that the entire point of making a syringe is for storage and/or transportation

it is steralized water with spores in it, exactly what i want to do!!

would someone with some experience(and a little bit of intelligence) please respond...

(although i could be very wrong[which i doubt], and in which case, i appolgize in advance)


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OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 12 years, 8 months
Re: other ways to rehydrate spores.... [Re: Ravinoff]
    #1729084 - 07/19/03 12:57 AM (14 years, 1 month ago)

The only way to pour it on substrate would require a whole lot of open air exposure after the sterlization which is a big risk of contaminats. Also if using PF tek u would have problems getting the water through the vermiculite shield layer (which is, I think, an important thing to have present). Maybe with grain and tyvek in a flowhood...but is it worth it?

Hence the syringe is for sterlity as well as storage or shipping.


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 11 months
Re: other ways to rehydrate spores.... [Re: TaoinShrrom]
    #1729206 - 07/19/03 02:03 AM (14 years, 1 month ago)

im going to use a jar with the cap having 1 hole with some polyfill in it...
http://www.fungifun.org/polyfill/

what if i were to put a second hole in it, tape it up untill im ready to put the spores in, then remove the tape, funnel the spores in, and close it in like 5 seconds? would that still be a big risk to contamination?

thanks for the help


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: other ways to rehydrate spores.... [Re: Ravinoff]
    #1729219 - 07/19/03 02:10 AM (14 years, 1 month ago)

there are other ways, but they are NOT simpler or easier .

Agar

Whole Grains

Start here and read, read, read !!


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OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 12 years, 8 months
Re: other ways to rehydrate spores.... [Re: deanofmean]
    #1729235 - 07/19/03 02:19 AM (14 years, 1 month ago)

If you limit the amount of exposure both the substrate and the spore water recieve to open air it could work. You would HAVE to use grains for this I think. I just don't think it would work well with BRF cakes or anything. This would probably require about the same amount of sterility as a g2g tranfer. You might want to try to make a positive pressure glove box. I know there is a photo guide around somewhere. I will look. But the design is simple, just take a tupper ware container. Cut holes on the side large enough for gloves, tape them up, then cut a hole on the other side, place a hepa filter on the outside and a computer fan on the inside to suck air into the container (hence positive airflow). Lysol well, seal everything up and do the pouring in that, I think you would have success.


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OfflineRavinoff
LethargicSnorting Duck

Registered: 07/15/03
Posts: 686
Last seen: 13 years, 11 months
Re: other ways to rehydrate spores.... [Re: TaoinShrrom]
    #1729809 - 07/19/03 10:38 AM (14 years, 1 month ago)

thanks for all the info!

one last question, is normal popcorn that i buy from a store(the kind that comes in a small bag and you put it in microwave to pop it) good enough?


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OfflineTaoinShrrom
The Action inInaction

Registered: 07/03/03
Posts: 98
Last seen: 12 years, 8 months
Re: other ways to rehydrate spores.... [Re: Ravinoff]
    #1729834 - 07/19/03 11:28 AM (14 years, 1 month ago)

Here is the photo guide for the glove box:
http://www.theomc.com/forums/index.cfm?CFID=2569816&CFTOKEN=33040801&fuseaction=read&forum=30&id=381

I just use the 79cent bag of yellow popcorn from walmart. One bag is enough for about 2 quart jars. I wouldn't use the microwavable popcorn simply because you dont get nearly as much for your money and I think it has a tendancy to be heavly salted (not good).


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Offlinedickmcplentie
Traveler
Registered: 06/27/03
Posts: 75
Loc: Orions Belt at the moment
Last seen: 14 years, 22 days
Re: other ways to rehydrate spores.... [Re: TaoinShrrom]
    #1729844 - 07/19/03 11:44 AM (14 years, 1 month ago)

That sounds like it would work if you could get a moderate level of sterility but wouldn't it be not only much more difficult, but a total waste of spores?

I bought appropriate syringes from the feed store for ~$1 a piece including needles. I have no experience so you didn't ask me but I think this is the standard because it is the best, easiest, most efficient way.

The glovebox is a good idea anyway, I've found out the hard way that I will be needing the extra level of protection from contams.


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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, HEPA Filter, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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