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SmokinErb
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100% Contam rate. Need advice on filter material.
#17200476 - 11/11/12 10:48 AM (12 years, 2 months ago) |
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Okay so a couple weeks ago, RR told me that tyvek envelopes are a "piss poor" filter material. So I cut up a tyvek lab suit to use as a filter.
-PC'd jars for 2 hours @ 15 psi.
-Inoculated about 25 jars, had another 10 that I hadn't inoculated yet. 100% contam rate.
I PC'd 'em again yesterday to kill everything and just disposed of them today. I'll be preparing more jars soon, and need a material that actually works. I'll probably go back to the envelopes for now, simply because I am on a budget and for the most part they've worked well for me.
Now, I know there are different grades of Tyvek, and that the envelope grade maybe "piss poor" but I don't know what makes a good quality tyvek for this hobby. Would something like this be better? http://menards.com/main/p-1868530-c-5824.htm
Any suggestions are welcome. Except for synthetic disks. $15 for a dozen isn't going to cut it until I run into some kind of money (I have roughly 200 jars)
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Vire
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200497 - 11/11/12 10:55 AM (12 years, 2 months ago) |
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Maybe double or even triple layer your Tyvek?
There are a lot of things that could cause contamination other than your filter media.
Can you tell us a bit more about your sterilizing procedures?
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toxetel
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Registered: 08/13/12
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200501 - 11/11/12 10:56 AM (12 years, 2 months ago) |
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Lab suit tyvek isn't a perfect filter, but you should be able to make it work with a more than 0% success rate. Recall that the original PF Tek doesn't even call for a filter beyond the dry verm layer. I would look at the rest of your process. Your sterile technique may need some work. Or the jars might need a better location to colonize. Or your inoculant may be contaminated.
Where did the contamination start in each jar? Are these BRF jars or grain jars? What kind of contam was it?
Synthetic filter discs are, in RR's words, the gold standard. If you buy large discs and cut small circles out of them to use to cover holes drilled in lids you can use one SFD for something like 8 lids.
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FunnyFungi
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Registered: 10/09/12
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Re: 100% Contam rate. Need advice on filter material. [Re: Vire]
#17200506 - 11/11/12 10:58 AM (12 years, 2 months ago) |
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are you using grain?
If so, are you prepping it correctly?
And for filters, i use a felt sheet bought at walmart.
Trace as many quarter sized sections you need to cover your GE hole on both sides.
30 jars= 60 quarter sized pieces of felt.
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TheApprentice
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Re: 100% Contam rate. Need advice on filter material. [Re: toxetel]
#17200510 - 11/11/12 10:58 AM (12 years, 2 months ago) |
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where did u get your syringe?
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Rahz
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200552 - 11/11/12 11:08 AM (12 years, 2 months ago) |
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Quote:
Okay so a couple weeks ago, RR told me that tyvek envelopes are a "piss poor" filter material. So I cut up a tyvek lab suit to use as a filter.

Tyvek has always performed well for me. If you have un-inoculated jars that went bad it's either the filter or the grain prep. Tell us how you prepared the grain prior to PCing and how you fixed the filters.
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shroom_bee
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200556 - 11/11/12 11:09 AM (12 years, 2 months ago) |
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I've never contamed a jar that I can attribute via lid.
I used to use tyvek rubber banded when I ran out of rings in the old evil PF Tek days. I since then RTV glued tyvek patches (1/4 of a dime, lots of rtv), and still use those lids (2+ years old).
And I used both postal and moon suit tyvek. No issues with either.
I've since moved to filter disks, but, against RR's advice (yes, I TRY to follow it, but hey, I got a bunch of lids already made, and they work), I cut them up and glue a 1/4 dime size piece in.
This was done before I read that it severely limits the GE since you don't get the whole surface/side area, even if you only have a little hole on one side. Also, you need to wash them, and NEVER use them when damp, so I put them in the dehydrator before filling jars and using them.
On the other hand, my typical jar time for rye or corn is 10 days, so I really don't think it affects me.
Not 1 contam due to noc up or shake out in the open for 10 days or so. A LOT of jars.
So, I'd REALLY look to something other than the tyvek for your issue.
Edited by shroom_bee (11/11/12 11:15 AM)
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Pinkus_Trip



Registered: 02/23/11
Posts: 492
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200597 - 11/11/12 11:21 AM (12 years, 2 months ago) |
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I have had the same thing happen with the tyvek filter. I got 10 jars of green mold on my popcorn and wbs but the same syringes worked 100% on my brf is polyfill any better than tyvek?
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SmokinErb
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Re: 100% Contam rate. Need advice on filter material. [Re: shroom_bee]
#17200607 - 11/11/12 11:23 AM (12 years, 2 months ago) |
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Whoa, lots of replies. Okay let me see how much I can clear up here.
These are milo grain jars. I prepare them by soaking for 24 hours, and then steeping to bring the moisture content to the proper level. Drain and spread over a screen until dry enough to jar. Usually an hour or two.
I then PC for 2 hours at 15 psi.
All of these jars were done G2G in my lab (PVC frame wrapped in plastic, HEPA intake). I average about 5-10% contam rate doing g2g in open air in my lab. The other jars were never inoculated, and just sat around in the incubation room.
On inoculated jars, contam appeared in various places of the grains, as they were shaken.
On uninoculated jars, all of the contam growth begins at the very top of the grains. This is why I'm led to believe that it was my filter.
Contam is cobweb/trich and some brown/yellow mold. Starts with a B, can't quite recall the name. At the moment.
Didn't use a syringe for these, but the syringe I used to inoculate the jars I did g2g with I made myself. Cloned a PE mushroom in LC, and tested the LC on "Agar" (BRF + water).
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total
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200614 - 11/11/12 11:24 AM (12 years, 2 months ago) |
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<--- Read under my name... Tyvek envelopes ftw...
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SmokinErb
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200616 - 11/11/12 11:25 AM (12 years, 2 months ago) |
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Okay. So I wanted to confirm that my coveralls were good material.
They're not even tyvek. It's "Tygard" material, I assumed it was an "off brand" tyvek. I was wrong.
I'm still wondering though, 100% contam rate? Well, 59/60 jars contaminated. I have ONE uninoculated jar that hasn't contaminated yet.
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SmokinErb
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200631 - 11/11/12 11:27 AM (12 years, 2 months ago) |
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For reference, do not use these coveralls as filter material. They can be found at Mendards, and are not worth shit.
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Pinkus_Trip



Registered: 02/23/11
Posts: 492
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb]
#17200668 - 11/11/12 11:37 AM (12 years, 2 months ago) |
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Quote:
SmokinErb said:

For reference, do not use these coveralls as filter material. They can be found at Mendards, and are not worth shit.
lol thats the same one i used i got it at wal-mart I was thanking it was the same thing
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SmokinErb
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Re: 100% Contam rate. Need advice on filter material. [Re: Pinkus_Trip]
#17200679 - 11/11/12 11:40 AM (12 years, 2 months ago) |
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Did you have god awful contam rates with it? Be great to hear from someone else that they had the same problem. Would at least eliminate that its my sterile procedure as a cause.
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Pinkus_Trip



Registered: 02/23/11
Posts: 492
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Re: 100% Contam rate. Need advice on filter material. [Re: Pinkus_Trip]
#17200687 - 11/11/12 11:42 AM (12 years, 2 months ago) |
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Quote:
Pinkus_Trip said: I have had the same thing happen with the tyvek filter. I got 10 jars of green mold on my popcorn and wbs but the same syringes worked 100% on my brf is polyfill any better than tyvek?
Yep all 10 jars= green mold
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Fischer
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Re: 100% Contam rate. Need advice on filter material. [Re: Pinkus_Trip]
#17301423 - 11/29/12 05:15 AM (12 years, 1 month ago) |
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Just buy some PTFE membrane filters... how's $35 per 100 discs sound?
http://www.aliexpress.com/item/47mm-PTFE-Membrane-Disc-Filters/291286021.html
Think of the time/substrate cost of contam'd jars and ask if you find it worth it to at least try it on some of your jars and see if it's the contamination vector you're having trouble with?
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RogerRabbit
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Re: 100% Contam rate. Need advice on filter material. [Re: Fischer]
#17301500 - 11/29/12 06:09 AM (12 years, 1 month ago) |
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Those aren't suitable. They react with alcohol and allow water/fluids to pass. We use thicker filters for air. Just grab synthetic filter disks. I've never paid over $40 per 100. RR
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c0ri


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Re: 100% Contam rate. Need advice on filter material. [Re: RogerRabbit]
#17301632 - 11/29/12 07:07 AM (12 years, 1 month ago) |
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I am using those exact coveralls for some g2g jars of Hautala currently and have a 0% contam rate. Seem to work fine for me. Not to mention they're colonizing quicker than my jars of maztapec that I did the same day, but used SFD's with those.
40$ for 100 discs is a great price! Where do you buy yours at RR? I paid around 40$ for 20 SFD's through myco supply, but was gouged on shipping at like 19.50$.
Edited by c0ri (11/29/12 07:10 AM)
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: 100% Contam rate. Need advice on filter material. [Re: c0ri]
#17301729 - 11/29/12 07:42 AM (12 years, 1 month ago) |
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Tygard is not tyvek.
I get my SFDs from the mad season. The 125mm are $1.75 each which is pricey as hell for each jar but I can cut 16 circles from each...comes to 11 cents per lid for a perfect sized filter.

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TheApprentice
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Re: 100% Contam rate. Need advice on filter material. [Re: FrankHorrigan]
#17301830 - 11/29/12 08:13 AM (12 years, 1 month ago) |
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Quote:
FrankHorrigan said: Tygard is not tyvek.
I get my SFDs from the mad season. The 125mm are $1.75 each which is pricey as hell for each jar but I can cut 16 circles from each...comes to 11 cents per lid for a perfect sized filter.
 

ive been considering moving to the plastic lids. i literally only do g2g's... can u find locally, or just online? my mailbox has issues
--------------------
RR Videos -Best $9 Ever Spent *
No Pour AGAR Tek * Easy COIR Trays! *
Pink Oysters on Newspaper TEK
"Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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skippydude
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Re: 100% Contam rate. Need advice on filter material. [Re: SmokinErb] 1
#17301936 - 11/29/12 08:41 AM (12 years, 1 month ago) |
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QUOTE "my lab (PVC frame wrapped in plastic, HEPA intake)."
_____________________________________________________-------------------------
Really surprised none of you vets caught this!
Unless you are using a flow hood or SAB in this setup, it is only semi-contam free until you enter. After entry, no matter how clean you think you are, your lab is contaminated.
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twistedty
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Re: 100% Contam rate. Need advice on filter material. [Re: FrankHorrigan]
#17302256 - 11/29/12 10:03 AM (12 years, 1 month ago) |
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Quote:
FrankHorrigan said: Tygard is not tyvek.
I get my SFDs from the mad season. The 125mm are $1.75 each which is pricey as hell for each jar but I can cut 16 circles from each...comes to 11 cents per lid for a perfect sized filter.
 
thats what my lid box looks like also, i use exact same tek but with metal, and since i only do agar wedges or g2g i have a single 1/2" hole for teh GE
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: 100% Contam rate. Need advice on filter material. [Re: twistedty]
#17302260 - 11/29/12 10:04 AM (12 years, 1 month ago) |
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Quote:
twistedty said:
thats what my lid box looks like also, i use exact same tek but with metal, and since i only do agar wedges or g2g i have a single 1/2" hole for teh GE
I made those with inoc ports years ago, I only do agar and G2G as well. But the lids still work nice 
I use a 1/4" hole for GE, seems to work great for me. Any benefit you see from 1/2" hole vs 1/4"?
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twistedty
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Re: 100% Contam rate. Need advice on filter material. [Re: FrankHorrigan]
#17302287 - 11/29/12 10:09 AM (12 years, 1 month ago) |
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Quote:
FrankHorrigan said:
Quote:
twistedty said:
thats what my lid box looks like also, i use exact same tek but with metal, and since i only do agar wedges or g2g i have a single 1/2" hole for teh GE
I made those with inoc ports years ago, I only do agar and G2G as well. But the lids still work nice 
I use a 1/4" hole for GE, seems to work great for me. Any benefit you see from 1/2" hole vs 1/4"?
i have seen no difference, ive used both but when i made my lids i followed this video
he uses 3/4" and i thought that was to much. and at the time i was using 1/4" so i just met in the middle with 1/2"
thats my logic
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skippydude
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Re: 100% Contam rate. Need advice on filter material. [Re: twistedty]
#17302317 - 11/29/12 10:15 AM (12 years, 1 month ago) |
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Quote:
twistedty said:
Quote:
FrankHorrigan said: Tygard is not tyvek.
I get my SFDs from the mad season. The 125mm are $1.75 each which is pricey as hell for each jar but I can cut 16 circles from each...comes to 11 cents per lid for a perfect sized filter.
 
thats what my lid box looks like also, i use exact same tek but with metal, and since i only do agar wedges or g2g i have a single 1/2" hole for teh GE
How do you get that red RTV to stick? Most of the ones I did like that came apart in the PC. I have to use full size SFD between the lid and ring, with a couple holes in the lid.
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twistedty
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Re: 100% Contam rate. Need advice on filter material. [Re: skippydude]
#17302326 - 11/29/12 10:17 AM (12 years, 1 month ago) |
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Quote:
skippydude said:
Quote:
twistedty said:
Quote:
FrankHorrigan said: Tygard is not tyvek.
I get my SFDs from the mad season. The 125mm are $1.75 each which is pricey as hell for each jar but I can cut 16 circles from each...comes to 11 cents per lid for a perfect sized filter.
 
thats what my lid box looks like also, i use exact same tek but with metal, and since i only do agar wedges or g2g i have a single 1/2" hole for teh GE
How do you get that red RTV to stick? Most of the ones I did like that came apart in the PC. I have to use full size SFD between the lid and ring, with a couple holes in the lid.
grab some sandpaper and rough up the plastic where your rtv or silicone is gonna be
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: 100% Contam rate. Need advice on filter material. [Re: skippydude]
#17302335 - 11/29/12 10:19 AM (12 years, 1 month ago) |
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Quote:
skippydude said:
How do you get that red RTV to stick? Most of the ones I did like that came apart in the PC. I have to use full size SFD between the lid and ring, with a couple holes in the lid.
Sandpaper the plastic around the hole first. For the inoc ports on those lids you see there, I use a shitload of RTV. Enough to fill the hole and have it bulging on both sides. then i smooth it out and let it cure for a full 24 hours.
If it's not fully cured and you PC it, it will generally fall apart. Same if you use just a little. During inoc, you don't want the needle to take the RTV port out with it either, which I've had happen when I used to little RTV.
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Fischer
Teonanacatalyst


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Posts: 565
Loc: Downunder
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Re: 100% Contam rate. Need advice on filter material. [Re: RogerRabbit]
#17306467 - 11/29/12 11:52 PM (12 years, 1 month ago) |
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Quote:
RogerRabbit said: Those aren't suitable. They react with alcohol and allow water/fluids to pass. We use thicker filters for air. Just grab synthetic filter disks. I've never paid over $40 per 100. RR
Eh? I might have missed something, but aren't what I posted PTFE (ie synthetic) micron filter discs?
I've never had contamination using them, they shouldn't react with ethanol in mycology applications and the ones I use are .45µm (0.45 micron) pore size, which IIRC is smaller than most/any mold spores and will also not allow bacteria to pass?
They will allow some liquids to pass under pressure (or when wetted with an alcohol, which I didn't realise), but the only liquid that would be relevant here would be water, which will not pass without wetting or pressure and even then will not allow any contaminants that would concern us to pass.
What do you mean by "synthetic filter discs", if not PTFE micron membranes?
Edited by Fischer (11/30/12 12:00 AM)
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shroom_bee
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Re: 100% Contam rate. Need advice on filter material. [Re: FrankHorrigan]
#17307154 - 11/30/12 04:41 AM (12 years, 1 month ago) |
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Quote:
FrankHorrigan said: During inoc, you don't want the needle to take the RTV port out with it either, which I've had happen when I used to little RTV.
Oh, yeah, f'ing HATE that. Also, I hate plastic lids. No matter how rough the sandpaper, or how much I use, the edges of the RTV peel up sooner or later. That shit it not meant to stick together.
I did the same thing as you on the filters, cut them into quarter size circles, and RTVed the hell out of them. Plastic peeled sooner or later, metal, on the other hand, lasted until it rusted away.
Edited by shroom_bee (11/30/12 04:44 AM)
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skippydude
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Re: 100% Contam rate. Need advice on filter material. [Re: shroom_bee]
#17307779 - 11/30/12 08:35 AM (12 years, 1 month ago) |
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You will not know if it is the filter. Until you do your G2G transfers in a sterile manner. Doing open air transfers is likely the problem. 
I have used band aids with 100% success. When done in the SAB. IMPO it is not your filter.
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Hioxify



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Re: 100% Contam rate. Need advice on filter material. [Re: c0ri]
#17308882 - 11/30/12 12:44 PM (12 years, 1 month ago) |
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I'm in the same predicament.I will most likely buy them from mycosupply unless I find another source. What are the red dots for, syringe puncture?
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TheCyndicate
Conglomerate



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Re: 100% Contam rate. Need advice on filter material. [Re: FrankHorrigan]
#17308976 - 11/30/12 01:04 PM (12 years, 1 month ago) |
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Quote:
FrankHorrigan said:
Quote:
skippydude said:
How do you get that red RTV to stick? Most of the ones I did like that came apart in the PC. I have to use full size SFD between the lid and ring, with a couple holes in the lid.
Sandpaper the plastic around the hole first. For the inoc ports on those lids you see there, I use a shitload of RTV. Enough to fill the hole and have it bulging on both sides. then i smooth it out and let it cure for a full 24 hours.
If it's not fully cured and you PC it, it will generally fall apart. Same if you use just a little. During inoc, you don't want the needle to take the RTV port out with it either, which I've had happen when I used to little RTV.
In my experience, When making lids with RTV you want to prepare your spawn and get ready to PC your jar's . Right before you load your jars in to your PC, apply the RTV where there is enough on both sides of the lid , put on jar , place band on , Foil and load in to PC. The intense heat of the PC will automatically cure the RVT , The higher the temp the faster it cures . IMO RTV sucks it only last like five rounds in the pressure cooker .
Cyn
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: 100% Contam rate. Need advice on filter material. [Re: TheCyndicate]
#17309264 - 11/30/12 01:54 PM (12 years, 1 month ago) |
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I've been using RTV to seal my filters to my plastic lids for over a year...haven't had to re-seal more than two or three of the hundred or so I've made. I let em cure for at least 24 before PCing. I do a lot of work too...they've been through countless PC cycles. I don't even sandpaper the lids first, to be honest. Maybe the RTV fairy just loves me more
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shroom_bee
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Re: 100% Contam rate. Need advice on filter material. [Re: TheCyndicate]
#17309273 - 11/30/12 01:54 PM (12 years, 1 month ago) |
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Quote:
TheCyndicate said:
In my experience, When making lids with RTV you want to prepare your spawn and get ready to PC your jar's . Right before you load your jars in to your PC, apply the RTV where there is enough on both sides of the lid , put on jar , place band on , Foil and load in to PC. The intense heat of the PC will automatically cure the RVT , The higher the temp the faster it cures . IMO RTV sucks it only last like five rounds in the pressure cooker .
Cyn
It sucks because you cured it that way. Give it a full 24 hour cure, NO MOISTURE OR PRESSURE, use metal lids, and you will find out the lids rusts from a couple of years of use (20-30 PC runs) before the RTV comes off.
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skippydude
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Re: 100% Contam rate. Need advice on filter material. [Re: shroom_bee]
#17309378 - 11/30/12 02:12 PM (12 years, 1 month ago) |
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Keep the RTV it has cost me one too many LCs.
Full size SFD for me or micro pore tape with heavy duty injection port for grain jugs.
And LC jugs like this
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TheCyndicate
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Re: 100% Contam rate. Need advice on filter material. [Re: shroom_bee]
#17309473 - 11/30/12 02:28 PM (12 years, 1 month ago) |
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Quote:
shroom_bee said:
Quote:
TheCyndicate said:
In my experience, When making lids with RTV you want to prepare your spawn and get ready to PC your jar's . Right before you load your jars in to your PC, apply the RTV where there is enough on both sides of the lid , put on jar , place band on , Foil and load in to PC. The intense heat of the PC will automatically cure the RVT , The higher the temp the faster it cures . IMO RTV sucks it only last like five rounds in the pressure cooker .
Cyn
It sucks because you cured it that way. Give it a full 24 hour cure, NO MOISTURE OR PRESSURE, use metal lids, and you will find out the lids rusts from a couple of years of use (20-30 PC runs) before the RTV comes off.
Hmmmm no is sucks because it's not made to be used as an inoculation port. What's this years it takes to rust altered lids. Once you alter and drill a hole in a metal lid and PC them 2-3 times they rust. I noticed this after my first pf-tek. I have given RTV 24 hours to cure before ..... and it still sucks . But hey some of my lids still have some RTV ports on them . Done use them anymore ... but they around here somewhere.
Cyn
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skippydude
Myco-curious



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Re: 100% Contam rate. Need advice on filter material. [Re: TheCyndicate]
#17309504 - 11/30/12 02:34 PM (12 years, 1 month ago) |
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PaperTiger
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Re: 100% Contam rate. Need advice on filter material. [Re: skippydude]
#17309730 - 11/30/12 03:15 PM (12 years, 1 month ago) |
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Skippy, I like your LC lids. How do the cultures perform? I'm assuming the syringe filter doubles as the gas exchange membrane as well? Any differences in growth, compared to lids with synthetic filter discs and a quarter inch hole? I've never worked with those species, not sure if GE requirements be much different. So when the time comes, you just glove box/flow hood them and quickly install the syringe? How are the contamination rates?
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skippydude
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Re: 100% Contam rate. Need advice on filter material. [Re: PaperTiger]
#17309801 - 11/30/12 03:26 PM (12 years, 1 month ago) |
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Quote:
PaperTiger said: Skippy, I like your LC lids. How do the cultures perform? I'm assuming the syringe filter doubles as the gas exchange membrane as well? Any differences in growth, compared to lids with synthetic filter discs and a quarter inch hole? I've never worked with those species, not sure if GE requirements be much different. So when the time comes, you just glove box/flow hood them and quickly install the syringe? How are the contamination rates?
For inoculation and drawing cultures I poke a new hole to avoid contamination.
Cultures perform well(have not lost 1 to faulty RTV)
Don't know if it is necessary but have been running 10-20cc air through the whatman weekly for extra oxygen.
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shroom_bee
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Re: 100% Contam rate. Need advice on filter material. [Re: TheCyndicate]
#17309986 - 11/30/12 03:58 PM (12 years, 1 month ago) |
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Quote:
TheCyndicate said: What's this years it takes to rust altered lids. Once you alter and drill a hole in a metal lid and PC them 2-3 times they rust. I noticed this after my first pf-tek.
Oh, yes, agreed, they will start to rust in a month or 2. But not at the drill points, those are covered with RTV (that seems to stick around for me). My rims need to be replaced far more often than my lids (6 months or so). But even the lids usable for years. As long as the myc doesn't mind the rust, neither do I. Until it goes to the edge and screws up the seal.
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TheCyndicate
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Re: 100% Contam rate. Need advice on filter material. [Re: shroom_bee]
#17310207 - 11/30/12 04:37 PM (12 years, 1 month ago) |
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Quote:
shroom_bee said:
Quote:
TheCyndicate said: What's this years it takes to rust altered lids. Once you alter and drill a hole in a metal lid and PC them 2-3 times they rust. I noticed this after my first pf-tek.
Oh, yes, agreed, they will start to rust in a month or 2. But not at the drill points, those are covered with RTV (that seems to stick around for me). My rims need to be replaced far more often than my lids (6 months or so). But even the lids usable for years. As long as the myc doesn't mind the rust, neither do I. Until it goes to the edge and screws up the seal.
Si , all I use for my grain jars is one hole about the size of a nail with Polly stuffed in them.
Cyn
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twistedty
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Re: 100% Contam rate. Need advice on filter material. [Re: TheCyndicate]
#17310212 - 11/30/12 04:38 PM (12 years, 1 month ago) |
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i use a 1/2 inch, with sfd
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skippydude
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Re: 100% Contam rate. Need advice on filter material. [Re: twistedty]
#17310521 - 11/30/12 05:30 PM (12 years, 1 month ago) |
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I use 1/2" hole with a full size SFD over the top of it
 Here is 2 SFD & 1 with a Band Aid. Both worked
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Kalypto
Psychonaut


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Re: 100% Contam rate. Need advice on filter material. [Re: skippydude]
#17310559 - 11/30/12 05:38 PM (12 years, 1 month ago) |
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that happened to me it was my inoculant
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