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Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 14 years, 10 months
6T WBS + 3 weeks = nothing
    #1708778 - 07/12/03 05:10 PM (14 years, 10 months ago)

afoaf followed 6T's WBS tek...

-soaked Hartz bird seed for 24 hours.
-drained WBS well using plastic collander
-filled quart jars 5/8 full
-used tyvek with lid rings and 2 coffee filters fastened with rubber bands
-PC'd jars @ 15 psi for 90 min
-let cool overnight and innoc'd 4 jars with PES Amazonian...half the jars with 1 cc and the other half with 2 cc's.
-incubated jars @ 85-86 degrees

afoaf has checked on the jars periodically and has not seen a sign of anything- mycelial growth or contams. It has been at least 3 weeks now and NOTHING...

any insight for afoaf?


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Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


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Offlineshirley knott
not my real name
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Registered: 11/11/02
Posts: 9,105
Loc: London Flag
Last seen: 1 year, 4 months
Trusted Cultivator
Re: 6T WBS + 3 weeks = nothing [Re: droogie75]
    #1708780 - 07/12/03 05:13 PM (14 years, 10 months ago)

if 3 weeks and no growth at all, could it be just an old or empty syringe?
any smell at all?


--------------------
buh


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OfflineJackal
Well Versed In Etiquette
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Registered: 10/16/02
Posts: 4,572
Last seen: 7 months, 26 days
Re: 6T WBS + 3 weeks = nothing [Re: droogie75]
    #1708785 - 07/12/03 05:14 PM (14 years, 10 months ago)

Something should have happened by now. What was the moisture content like after PCing? Where did the spores come from? Reputable source? It may also be an invisible contam.


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Invisiblegeorge castanzaM
Lord Of The Idiots!
I'm a teapot User Gallery Arcade Champion: BurgerTime, Space Invaders
Registered: 10/21/02
Posts: 7,542
Trusted Cultivator
Re: 6T WBS + 3 weeks = nothing [Re: Jackal]
    #1708986 - 07/12/03 06:18 PM (14 years, 10 months ago)

if none of your jars did shit it sounds like a bunk syringe to kramer



kramer also likes your posting style (lots of info)
shrooms for you


--------------------
Kramer Cakes


[url=[url=

   

:pm:


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Offlinehyper_dermic
stranger withcandy

Registered: 06/26/02
Posts: 736
Loc: the land of excess
Last seen: 13 years, 8 months
Re: 6T WBS + 3 weeks = nothing [Re: droogie75]
    #1709007 - 07/12/03 06:26 PM (14 years, 10 months ago)

yup they are absolutely correct....
theres only 2 ways this tek could have failed....

either your grain was too wet.. leading to a contam...
or the syringe was crappy...

[hyp]


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Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 14 years, 10 months
Re: 6T WBS + 3 weeks = nothing [Re: george castanza]
    #1709645 - 07/12/03 09:36 PM (14 years, 10 months ago)

thanks for the shrooms... :laugh:

 


--------------------
Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


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InvisibleAinasko
Oksania
Registered: 04/26/03
Posts: 694
Re: 6T WBS + 3 weeks = nothing [Re: droogie75]
    #1709667 - 07/12/03 09:45 PM (14 years, 10 months ago)

You mentioned that your jars were just Tyvek, lid rings and coffee filters. Was a lid with a few holes included or just the filters?

It's possible that your substrate dried before colonization began.


--------------------
Ainasko is my name backwards. I'm a girl!


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