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Offlineflexorman
Stranger

Registered: 07/18/12
Posts: 57
Last seen: 11 years, 7 months
Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #17083252 - 10/23/12 02:20 AM (12 years, 2 months ago)

My agar got contamination(brown)but i let the mycelium grow and now mycelium is taking over the jar,can i use this agar or transfer it to new agar plate and wait to see if there is still contamination?

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Offlinedude1787
requires a pickle hat


Registered: 10/06/12
Posts: 378
Loc: Canada
Last seen: 12 years, 1 month
Re: I want to see pictures of your agar work. [Re: flexorman]
    #17083260 - 10/23/12 02:23 AM (12 years, 2 months ago)

Got any pictures?


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InvisibleExiledmonkies
Peaceful Warrior
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Registered: 12/04/11
Posts: 514
Re: I want to see pictures of your agar work. [Re: dude1787]
    #17083394 - 10/23/12 03:22 AM (12 years, 2 months ago)

Quote:

dude1787 said:
Just wondering what the genetically best stuff would look like, or if repeatedly isolating would somehow lead to getting a better strain.



Evaluating a strain is a lengthy process.

How fast your isolation recovers from the shock of inoculation.
How fast your mycelium grows.
How strongly the mycelial mat grows.
How long it takes from colonization to fruiting.
How many primordia form and grow into full fruits.
How much does primordia formation decrease on your 2nd, 3rd, and 4th flush and how many flushes do you get...etc...etc

Like I said make backups of your work because you could come across a strain you think is amazing then find out it is genetically unstable. (a couple grows down the line and the mutants appear or primordia formation because fewer and fewer)

Edited by Exiledmonkies (10/23/12 03:42 AM)

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Offlinedude1787
requires a pickle hat


Registered: 10/06/12
Posts: 378
Loc: Canada
Last seen: 12 years, 1 month
Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #17083411 - 10/23/12 03:32 AM (12 years, 2 months ago)

Awesome! thanks, seriously. I was thinking of doing a little isolating, Going from the first plate and then to a second and maybe a third and eventually cloning my fastest growing, strongest and largest fruits..  Thanks again man.


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InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: I want to see pictures of your agar work. [Re: dude1787]
    #17083487 - 10/23/12 04:32 AM (12 years, 2 months ago)

Quote:

dude1787 said:
I don't fully understand isolating yet so I'm doing my homework on it but I have a pretty good idea how it works. Any advice would be appreciated.




THIS thread should be helpful.


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Quick WBS Prep

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OfflineFonzee
Pretzel
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Registered: 07/25/11
Posts: 512
Loc: Israel
Last seen: 1 year, 1 month
Re: I want to see pictures of your agar work. [Re: FooMan]
    #17103323 - 10/26/12 03:27 AM (12 years, 2 months ago)

A mispoured agar plate contaminated

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OfflineQueen of Kings
Get on with the Fascination
Female User Gallery


Registered: 12/11/11
Posts: 1,201
Loc: Pacific Northwest, almost
Last seen: 8 years, 6 days
Re: I want to see pictures of your agar work. [Re: Fonzee]
    #17103668 - 10/26/12 07:11 AM (12 years, 2 months ago)

Agar is where it's at. 

I am on a third transfer of this Penis Envy plate and really like the sectoring I'm getting.  In fact I've just taken a couple spots to a 4th transfer. 


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: I want to see pictures of your agar work. [Re: Queen of Kings]
    #17140941 - 11/01/12 08:02 AM (12 years, 2 months ago)

Beautiful pics!
Here are some of my own:


Mazatapec, 3rd transfer. Isolate? I'm not good at being able to spot them yet.


Penis Envy, 3rd transfer. Isolate?  Unsure again.


Z-Strain, 3rd transfer, isolate.


Z-strain, 3rd transfer, looking all sorts of crazy.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflineKingCloyster
Forest Mushroom 🍄
Male User Gallery


Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 2 years, 5 days
Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #19005877 - 10/20/13 08:48 PM (11 years, 2 months ago)

Quote:

Exiledmonkies said:
chanterelle isolates-


Shiitake isolates-





Bacteria contam from my first ever agar clone attempt-


Will get more up ASAP. Any questions on procedures just give me a shout. :smile:




Im gunna shout at ya real quick... that last pic of the bacterial contam that you have, the milky one.  Well i get that everytime i try agar.  sometimes white, sometimes pink. what is this caused by? Ive tried 10 attempts with petri dishes. all contamed.  Idk what I should do. I cant get a single mycelium specimen... Always contamed


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:sporedrop::sporedrop::mushdance::mushdance::sporedrop::sporedrop:

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