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InvisibleFrankHorrigan
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Agar pictures thread, post your agar pics
    #17076882 - 10/22/12 12:04 AM (12 years, 2 months ago)

I'm working on refining my agar work and would like to see pictures of agar work that you have done too. 
I'm still learning about sectors, isolating, etc. so anything you have to tell me about the pictures would be helpful as well!
Cubensis, edibles, medicinals, contams, whatever!  I wish there were more pictures of agar work available is all.

Here are some of my own:


Mazatapec, 3rd transfer.


Penis Envy, 3rd transfer. Isolate?  Unsure.


Z-Strain, 3rd transfer, isolate.


Z-strain, 3rd transfer, looking all sorts of crazy.
:awejeez:


--------------------

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Here is how I get things done.
You should take a look. :hehehe:


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AMU- Get an answer here -AMU

Edited by FrankHorrigan (11/01/12 08:26 AM)

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OfflineMiNdTwIsTeR
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Re: I want to see pictures of your agar work. [Re: FrankHorrigan]
    #17076993 - 10/22/12 12:37 AM (12 years, 2 months ago)

I too would like to see this! I just tried my first agar petris and outta 7 four are DEF contaminated and three are questionable.


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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: FrankHorrigan]
    #17077063 - 10/22/12 01:03 AM (12 years, 2 months ago)

I will be following this thread too as I'm just getting into agar work and.


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OfflinePussyFart
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Re: I want to see pictures of your agar work. [Re: dude1787]
    #17077110 - 10/22/12 01:28 AM (12 years, 2 months ago)

there is a PE6 isolate in my signature

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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: PussyFart]
    #17077120 - 10/22/12 01:31 AM (12 years, 2 months ago)

how long did it take it to start growing? just curious, was it multispore?


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Offlinetwistedty
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Re: I want to see pictures of your agar work. [Re: PussyFart]
    #17077137 - 10/22/12 01:36 AM (12 years, 2 months ago)

ive used agar for lil bit doing clones etc, but now that i have practice and more of an understanding of it im starting over.

this is the spores im starting with to start isolating and gettin some slants going.



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OfflinePussyFart
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Re: I want to see pictures of your agar work. [Re: dude1787]
    #17077165 - 10/22/12 01:59 AM (12 years, 2 months ago)

Quote:

Notahacker420 said:
there is a PE6 isolate in my signature



Quote:

dude1787 said:
how long did it take it to start growing? just curious, was it multispore?



That was a tissue sample from a MS PF-tek grow from a big fruit on one of my cakes, took about 5 transfers and I have 6 other isolates besides that one of that clone, fruiting them each out next to eliminate the less desirable ones. Surprisingly there was no rhisomorphic growth from this fruit.

Edited by PussyFart (10/22/12 02:00 AM)

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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: PussyFart]
    #17077186 - 10/22/12 02:18 AM (12 years, 2 months ago)

Whats the best stuff to isolate?


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InvisibleFooMan
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Re: I want to see pictures of your agar work. [Re: dude1787]
    #17077351 - 10/22/12 04:25 AM (12 years, 2 months ago)

Quote:

dude1787 said:
Whats the best stuff to isolate?




For cubes, I like this stuff:



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Quick WBS Prep

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Offlinetwistedty
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Re: I want to see pictures of your agar work. [Re: FooMan]
    #17077354 - 10/22/12 04:27 AM (12 years, 2 months ago)

Quote:

FooMan said:
Quote:

dude1787 said:
Whats the best stuff to isolate?




For cubes, I like this stuff:







love it

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InvisibleFrankHorrigan
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Re: I want to see pictures of your agar work. [Re: FooMan]
    #17077620 - 10/22/12 07:25 AM (12 years, 2 months ago)

Quote:

FooMan said:
Quote:

dude1787 said:
Whats the best stuff to isolate?




For cubes, I like this stuff:






That is beautiful. Now, is that a single sector that I'm looking at, or two sectors?
Also, my PE uncut plates never get very rhizomorphic for me. Generally it's very thick, puffy myc. Very nice work :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflineBoomhower
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Re: I want to see pictures of your agar work. [Re: FrankHorrigan]
    #17077744 - 10/22/12 08:13 AM (12 years, 2 months ago)


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Invisiblebillb
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Re: I want to see pictures of your agar work. [Re: Boomhower]
    #17077787 - 10/22/12 08:27 AM (12 years, 2 months ago)

The first pic is of the first agar project I did. I poured it too thick and the specimen was not the best. After about 3 or 4 transfers, the end result was not to bad. I didn't get a chance to take a pic of the agar before I transferred it to some rye, but here it is on the rye. It's amazing to see the difference between the beginning and end results of a agar project. 

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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: FooMan]
    #17079334 - 10/22/12 02:10 PM (12 years, 2 months ago)

Quote:

FooMan said:
Quote:

dude1787 said:
Whats the best stuff to isolate?




For cubes, I like this stuff:






Ok, thanks. Now I know what to look for.


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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: MiNdTwIsTeR]
    #17083113 - 10/23/12 01:17 AM (12 years, 2 months ago)

Wanted to say thanks to everyone on this thread for being so helpful. My first agar plates are starting to show growth today, little mycelium dots all over the place with No contams yet.


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Edited by dude1787 (10/23/12 01:18 AM)

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InvisibleExiledmonkies
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Re: I want to see pictures of your agar work. [Re: dude1787]
    #17083136 - 10/23/12 01:30 AM (12 years, 2 months ago)

This is one of my first spore iso projects.
http://www.shroomery.org/forums/showflat.php/Number/17051099

I thought the white was mold but its just Monokaryotic mycelium.

I'll get a lot more up in the next 24hrs if you want. I have agar work from dishes and tubes. (mainly clones)

Will be doing another 100-300 isolates shortly as well since I have a bunch of colonized dishes.

Have you done any iso work yet? Do you know how to pick the best part of the dish?

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InvisibleExiledmonkies
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Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #17083218 - 10/23/12 02:06 AM (12 years, 2 months ago)

chanterelle isolates-


Shiitake isolates-





Bacteria contam from my first ever agar clone attempt-


Will get more up ASAP. Any questions on procedures just give me a shout. :smile:

Edited by Exiledmonkies (10/23/12 02:12 AM)

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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #17083222 - 10/23/12 02:08 AM (12 years, 2 months ago)

I don't fully understand isolating yet so I'm doing my homework on it but I have a pretty good idea how it works. Any advice would be appreciated.

Also I give up on cakes too lolz but they were a great learning experience and still have their uses.


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Edited by dude1787 (10/23/12 02:13 AM)

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InvisibleExiledmonkies
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Re: I want to see pictures of your agar work. [Re: dude1787]
    #17083241 - 10/23/12 02:17 AM (12 years, 2 months ago)

Quote:

dude1787 said:
I don't fully understand isolating yet so I'm doing my homework on it but I have a pretty good idea how it works. Any advice would be appreciated.

Also I give up on cakes too lolz but they were a great learning experience and still have their uses.


What parts do you not understand? Isolating from spores or from a fresh mushroom?

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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #17083250 - 10/23/12 02:20 AM (12 years, 2 months ago)

Just wondering what the genetically best stuff would look like, or if repeatedly isolating would somehow lead to getting a better strain.


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Offlineflexorman
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Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #17083252 - 10/23/12 02:20 AM (12 years, 2 months ago)

My agar got contamination(brown)but i let the mycelium grow and now mycelium is taking over the jar,can i use this agar or transfer it to new agar plate and wait to see if there is still contamination?

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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: flexorman]
    #17083260 - 10/23/12 02:23 AM (12 years, 2 months ago)

Got any pictures?


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InvisibleExiledmonkies
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Re: I want to see pictures of your agar work. [Re: dude1787]
    #17083394 - 10/23/12 03:22 AM (12 years, 2 months ago)

Quote:

dude1787 said:
Just wondering what the genetically best stuff would look like, or if repeatedly isolating would somehow lead to getting a better strain.



Evaluating a strain is a lengthy process.

How fast your isolation recovers from the shock of inoculation.
How fast your mycelium grows.
How strongly the mycelial mat grows.
How long it takes from colonization to fruiting.
How many primordia form and grow into full fruits.
How much does primordia formation decrease on your 2nd, 3rd, and 4th flush and how many flushes do you get...etc...etc

Like I said make backups of your work because you could come across a strain you think is amazing then find out it is genetically unstable. (a couple grows down the line and the mutants appear or primordia formation because fewer and fewer)

Edited by Exiledmonkies (10/23/12 03:42 AM)

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Offlinedude1787
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Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #17083411 - 10/23/12 03:32 AM (12 years, 2 months ago)

Awesome! thanks, seriously. I was thinking of doing a little isolating, Going from the first plate and then to a second and maybe a third and eventually cloning my fastest growing, strongest and largest fruits..  Thanks again man.


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InvisibleFooMan
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Re: I want to see pictures of your agar work. [Re: dude1787]
    #17083487 - 10/23/12 04:32 AM (12 years, 2 months ago)

Quote:

dude1787 said:
I don't fully understand isolating yet so I'm doing my homework on it but I have a pretty good idea how it works. Any advice would be appreciated.




THIS thread should be helpful.


--------------------

Quick WBS Prep

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OfflineFonzee
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Re: I want to see pictures of your agar work. [Re: FooMan]
    #17103323 - 10/26/12 03:27 AM (12 years, 2 months ago)

A mispoured agar plate contaminated

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OfflineQueen of Kings
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Re: I want to see pictures of your agar work. [Re: Fonzee]
    #17103668 - 10/26/12 07:11 AM (12 years, 2 months ago)

Agar is where it's at. 

I am on a third transfer of this Penis Envy plate and really like the sectoring I'm getting.  In fact I've just taken a couple spots to a 4th transfer. 


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InvisibleFrankHorrigan
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Re: I want to see pictures of your agar work. [Re: Queen of Kings]
    #17140941 - 11/01/12 08:02 AM (12 years, 2 months ago)

Beautiful pics!
Here are some of my own:


Mazatapec, 3rd transfer. Isolate? I'm not good at being able to spot them yet.


Penis Envy, 3rd transfer. Isolate?  Unsure again.


Z-Strain, 3rd transfer, isolate.


Z-strain, 3rd transfer, looking all sorts of crazy.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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OfflineKingCloyster
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Re: I want to see pictures of your agar work. [Re: Exiledmonkies]
    #19005877 - 10/20/13 08:48 PM (11 years, 2 months ago)

Quote:

Exiledmonkies said:
chanterelle isolates-


Shiitake isolates-





Bacteria contam from my first ever agar clone attempt-


Will get more up ASAP. Any questions on procedures just give me a shout. :smile:




Im gunna shout at ya real quick... that last pic of the bacterial contam that you have, the milky one.  Well i get that everytime i try agar.  sometimes white, sometimes pink. what is this caused by? Ive tried 10 attempts with petri dishes. all contamed.  Idk what I should do. I cant get a single mycelium specimen... Always contamed


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