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justlookin
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Registered: 10/15/12
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uvc germicide for growth chamber ???
#17035399 - 10/15/12 01:39 PM (11 years, 4 months ago) |
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First post,.. I have some thoughts of proposing the use of a 9w uv-c bulb within the fruiting chamber for and usage beginning on the day casing is applied.
By using only 2 times a day for 3-5 minutes until pinheads form its my belief that this will benefit by preventing air-born contaminants and mold spreading on the casing layer.
Would it be possible to use the uv-c bulb in shorter instances relevant to the growth cycle of the unwanted contaminates to reduce the damage to the mycelium growth ?
If anyone has information relative please hmb or msg me. Thanks
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Jimbolino
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Re: uvc germicide for growth chamber ??? [Re: justlookin]
#17035557 - 10/15/12 02:09 PM (11 years, 4 months ago) |
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Why would want to do it? The casing itself should be non-nutrient and should thus be not prone to give soil for contams..
Pasteurize your casing and leave the uv out of your fc. Imho you will make more damage than getting benefits from it...
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Amanita virosa
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Re: uvc germicide for growth chamber ??? [Re: Jimbolino]
#17048472 - 10/17/12 01:31 PM (11 years, 3 months ago) |
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completely unnecessary! As the above poster said, the casing is not nutritive and is not subject to contamination so long as you STERILIZE it first. Always sterilize it like you would grain, unless you are dealing with a non cube species that needs bacteria to form primordia, in which case pasturization is acceptable. Otherwise STERILIZE THAT SHIT!
Edited by Amanita virosa (10/17/12 01:32 PM)
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shroom_bee
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Re: uvc germicide for growth chamber ??? [Re: Amanita virosa]
#17057523 - 10/18/12 08:06 PM (11 years, 3 months ago) |
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Quote:
Amanita virosa said: completely unnecessary! As the above poster said, the casing is not nutritive and is not subject to contamination so long as you STERILIZE it first. Always sterilize it like you would grain, unless you are dealing with a non cube species that needs bacteria to form primordia, in which case pasturization is acceptable. Otherwise STERILIZE THAT SHIT! 
Huh. I thought the casing was somewhat nutritive. Tric grows on it happily unless you lime it up. Of course, tric grows on everything. I've had certain mycs devour it, while others hung on the bottom, refusing to grow into it.
I was under the impression you pasteurize casing (50/50+ in my case) to leave it colonized with something already rather than leave it sterile and invite something far worse. As well as helping pin some difficult strains, but that is as much for the non-colonized airflow area as the bacteria.
So anyway, yeah, UVC will probably fuck up the myc far more than be helpful.
Unless of course you find out we are all wrong. Do a comparative run of identical myc, 1 with UV, 1 without, let us know.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,746
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Re: uvc germicide for growth chamber ??? [Re: Amanita virosa]
#17057630 - 10/18/12 08:23 PM (11 years, 3 months ago) |
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Quote:
Amanita virosa said: completely unnecessary! As the above poster said, the casing is not nutritive and is not subject to contamination so long as you STERILIZE it first. Always sterilize it like you would grain, unless you are dealing with a non cube species that needs bacteria to form primordia, in which case pasturization is acceptable. Otherwise STERILIZE THAT SHIT! 
If you sterilise your casing material there is a greater chance that it will become contaminated compared with a casing material that has been properly pasteurised, sterilising it can work but it is always better to correctly pasteurise it regardless of what species you are growing...
edit... Regarding the UVC, I wouldn't play with those, just pasteurise your casing material properly and you should have no problems with contamination in the casing layer.
Edited by inski (10/18/12 08:38 PM)
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Amanita virosa
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Re: uvc germicide for growth chamber ??? [Re: inski]
#17060282 - 10/19/12 09:34 AM (11 years, 3 months ago) |
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Quote:
inski said:
Quote:
Amanita virosa said: completely unnecessary! As the above poster said, the casing is not nutritive and is not subject to contamination so long as you STERILIZE it first. Always sterilize it like you would grain, unless you are dealing with a non cube species that needs bacteria to form primordia, in which case pasturization is acceptable. Otherwise STERILIZE THAT SHIT! 
If you sterilise your casing material there is a greater chance that it will become contaminated compared with a casing material that has been properly pasteurised, sterilising it can work but it is always better to correctly pasteurise it regardless of what species you are growing...
Agreed. There are advantages to pasturizing over sterilizing; namely, certain thermophillic bacteria and fungi survive the process and can "protect" the casing. Certain other contams may also survive though; namely, aspergillus species, which are resistant up to 150 degrees at 45 minutes pasturization time. This would be the threshhold for some beneficials as well. I have always sterilized my casing and never had contam issues. And so long as you are casing uncomtaminated spawn, you should not have an issue either way, as the myc should grow thru the casing well before any contam is observed. If not, some other parameter is not being well controlled.
Edited by Amanita virosa (10/19/12 09:52 AM)
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RogerRabbit
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Re: uvc germicide for growth chamber ??? [Re: Amanita virosa]
#17063999 - 10/19/12 08:34 PM (11 years, 3 months ago) |
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Quote:
as the myc should grow thru the casing well before any contam is observed.
The problem is you don't want the mycelium to grow through the casing. A casing layer if used, is most effective when it's barely colonized. This way, the uncolonized casing material on top has lots of little air pockets which are great places for primordia to form.
Since we don't want the casing layer to fully colonize, pasteurization is much better. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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justlookin
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Re: uvc germicide for growth chamber ??? [Re: RogerRabbit]
#17067779 - 10/20/12 02:56 PM (11 years, 3 months ago) |
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the casing has been psi @ 15 for 1 hour.. but there is still green mold from time to time usually from inadequate air flow or to much moisture.. so im looking for alternative routes to help prevent this from gaining a foothold on the casing.. also this method could possible be used to rid the chamber of contaminates in between cycles when not in use.
im not referring to a simple fc design.
Im also designing a climate control system that may spark allot of interest for those who may be interested.
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inski
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Re: uvc germicide for growth chamber ??? [Re: justlookin]
#17068294 - 10/20/12 04:26 PM (11 years, 3 months ago) |
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Pasteurise your casing material and you will have very little if any problems with green mold.
By sterilising the material you are creating a great habitat for contaminants to get a foot hold, by pasteurising properly the material will retain a multutude of microorganisms that are beneficial to fructification and also are great at preventing contamination.
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justlookin
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Re: uvc germicide for growth chamber ??? [Re: inski]
#17068406 - 10/20/12 04:41 PM (11 years, 3 months ago) |
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i understand the difference and see the benefits so basically as RR stated in another post:
You want to make sure the center of the jar reaches at least 140F and stays there for an hour, but don’t allow it to exceed 170F.
that is essentially the difference correct?
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inski
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Re: uvc germicide for growth chamber ??? [Re: justlookin]
#17068538 - 10/20/12 05:04 PM (11 years, 3 months ago) |
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That's correct, I think 160f is probably better though, between 140-160f.
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justlookin
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Re: uvc germicide for growth chamber ??? [Re: inski]
#17068571 - 10/20/12 05:12 PM (11 years, 3 months ago) |
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thanks.. im still dreaming of a way to use uv lol.
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inski
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Re: uvc germicide for growth chamber ??? [Re: justlookin]
#17068621 - 10/20/12 05:24 PM (11 years, 3 months ago) |
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No problem, I guess I can't say anything to make you change your mind about using uvc but do some research, I think it is known to cause genetic mutations in many organisms, in my opinion it is not safe to play with and there are better alternatives.
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Standardhepafilter
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Registered: 10/21/12
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Re: uvc germicide for growth chamber ??? [Re: justlookin]
#17071915 - 10/21/12 10:02 AM (11 years, 3 months ago) |
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Quote:
justlookin said: thanks.. im still dreaming of a way to use uv lol.
UV-bulbs are useful tools for surface sterilization. It is used in industial laminar flows to keep the stainless-steel surface in the laminar flow sterile.
But for home-growers it is much better, easier and safer to use desinfectants. UV-radiation can cause a sunburn or even cancer!
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Mycelio
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Plus it will damage your eyes.
Carsten
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RogerRabbit
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Re: uvc germicide for growth chamber ??? [Re: justlookin]
#17073757 - 10/21/12 03:23 PM (11 years, 3 months ago) |
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This thread has been closed.
Reason: I let this go on long enough, especially considering it's not an advanced mycology topic. There is no reason to have any kind of antimicrobial action in a fruiting chamber, but especially not UV. RR
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