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Invisiblemodern.shaman
San Mescalito
I'm a teapot


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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: Big L]
    #17018512 - 10/12/12 01:12 PM (11 years, 3 months ago)

Yea I saw that today it was on Cactus tissue culture also read part of that thread on here. Very cool stuff but went WAY over my head.

http://www.lapshin.org/cultivar/N34/in-vitro-e.htm


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Invisiblepsi
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: modern.shaman]
    #17025754 - 10/13/12 07:27 PM (11 years, 3 months ago)

Posting to subscribe, great topic.


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Invisiblemodern.shaman
San Mescalito
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: psi] * 1
    #17026235 - 10/13/12 08:46 PM (11 years, 3 months ago)

Well I had a few extra Lophophora Williamsii pups so yesterday I decided to graft a few on small stocks I had. I grafted 1 onto a Opuntia subulata monstrose, 2 on small pedro cuttings, another one a larger unrooted pedro cutting, and lastly 2 more on wild opuntia stricta (not sure if its infected or not).

The growth will take a few weeks since the temperatures are dropping and these are SMALL unrooted stocks. I'll update in a few weeks with new pictures of growth.

In the future I would like to try grafting on a lophocereus schotti monstrose since they are relatively cheap and easy to find.

The grafts seem to have already healed and taken however I will leave the pressure on them for 2-3 more days just as a precaution.


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Offlineintelligentlife
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: modern.shaman]
    #17027246 - 10/14/12 12:33 AM (11 years, 3 months ago)

Can anyone know do Opuntia Robusta carry any viruses? I have spineless one and I could get few stocks from it.

Can I infect trichcereus plant by rooting something virus inside cactus? Then it's possibility to keep infected tricho as stock?

Do anyone know any about spineless opuntia robusa, is it virus free cactus?


Are this cactus even worth anything with grafting? I have never grafted to opuntias anyway.:awesome:


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Invisiblemodern.shaman
San Mescalito
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: intelligentlife]
    #17030628 - 10/14/12 04:33 PM (11 years, 3 months ago)

Opuntias are worth grafting to because they are water tolerant and also have quick growth though it's not as fast as trichocereus or pereskiopsis.

As far as I know all Opuntia are very prone to infections so its worth a shot... they are usually visible showing rings or different colored patches on the skin.

Depending on the virus/infection the cactus needs to remain grafted or will revert. Others like the HAKUUN markings will not revert even if degrafted. You can also transfer some infections by not cleaning the blade after making the cut. http://www.shroomery.org/forums/showflat.php/Number/17017013#17017013


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Offlinetrippertom
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: modern.shaman]
    #17056904 - 10/18/12 06:28 PM (11 years, 3 months ago)

So I have two grafting stalk that would be perfect to try I think. I just can't seem to find any loaph seed right now any pointers


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Invisibleferrel_human
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: trippertom]
    #17056924 - 10/18/12 06:31 PM (11 years, 3 months ago)

Quote:

trippertom said:
So I have two grafting stalk that would be perfect to try I think. I just can't seem to find any loaph seed right now any pointers




one pointer, dont use that for stock.

:goodluck:


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Nature is my church and walking through it is gospel. It tells no lies and reveals all to those who look, and listen, closely.
-Karode


Looking for Mimosa tenuiflora seeds. Buttons for trade


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Offlinetrippertom
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: ferrel_human]
    #17056997 - 10/18/12 06:41 PM (11 years, 3 months ago)

Ok then.. so what's a good place to start just getting into cacti growing and don't know to much


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Invisibleferrel_human
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: trippertom]
    #17057377 - 10/18/12 07:40 PM (11 years, 3 months ago)

Quote:

trippertom said:
Ok then.. so what's a good place to start just getting into cacti growing and don't know to much





you've come to the right room.

now start threads young jedi.:jedi:


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Nature is my church and walking through it is gospel. It tells no lies and reveals all to those who look, and listen, closely.
-Karode


Looking for Mimosa tenuiflora seeds. Buttons for trade


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Offlinetrippertom
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: ferrel_human]
    #17057981 - 10/18/12 09:20 PM (11 years, 3 months ago)

This sounds more interesting then just growing active cacti


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Offlineintelligentlife
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: modern.shaman]
    #17059753 - 10/19/12 06:46 AM (11 years, 3 months ago)

Quote:

modern.shaman said:
Opuntias are worth grafting to because they are water tolerant and also have quick growth though it's not as fast as trichocereus or pereskiopsis.

As far as I know all Opuntia are very prone to infections so its worth a shot... they are usually visible showing rings or different colored patches on the skin.

Depending on the virus/infection the cactus needs to remain grafted or will revert. Others like the HAKUUN markings will not revert even if degrafted. You can also transfer some infections by not cleaning the blade after making the cut. http://www.shroomery.org/forums/showflat.php/Number/17017013#17017013




Is it possible that this opuntia carry infections/viruses cause there are huge damage below from mealybugs. They spread diseases and viruses aye?

So there is possibility that this opuntia robusta have somekind of infections cause the "scar" what mealybugs have left behind is almost size of one big plate. So there has been big colony of mealybugs year or two ago.

My opuntia robusta making now new grown so maybe I take it as for stock and look how's it going. I have few loph buttons coming what I will use for graft. But not so much trichos than small lophs.

Is it easy to graft several lophophora to one big opuntia robusta cutting? I was thinking to take the new offshoot when its littlebit bigger and use it for stock before it turns to "adult"-form. anyone has grafted lophs to opuntia robusta? or do I use few trichocereus cuttings to make graft and leave opuntia robusta alone?:shrug:


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Offlineintelligentlife
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: intelligentlife]
    #17059777 - 10/19/12 06:58 AM (11 years, 3 months ago)

One thing what I have thinking long time..

Is there any chemicals/toxins/etc what can make cactus grows different than normal?

I know some chemicals can cause plants to change form of growing and so on, but do anyone have find/tested anything this way?

Chemically poisoned specimens what maybe can make "damage" to DNA to make plant growing un-natural way?

I would be interested to try but first of all, I need to know some chemicals what can "harm" the plant to make it grow like it should not. Mutant formed by chemicals. Any ideas?:awesome:


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Invisiblemodern.shaman
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: intelligentlife]
    #17061003 - 10/19/12 11:58 AM (11 years, 3 months ago)

The closest I have seen to damaging the DNA of the plant causing abnormalities is this... http://www.shroomery.org/forums/showflat.php/Number/17018137#17018137

As far as chemicals go I have seen members post using BAP to induce pupping at a 'monstrous' level. http://www.thenook.org/forum/index.php?showtopic=58223&st=0

Next month I will try two different stresses... freezing for 5 minutes and microwaving for a few seconds. I have a lot of bridgesii seeds to experiment with so it wont be a problem to lose some or all of them in the name of science.


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Offlineintelligentlife
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: modern.shaman]
    #17061634 - 10/19/12 01:53 PM (11 years, 3 months ago)

How hard these chemicals are to get in europe?  Anyone knows?:rolleyes:


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Invisiblemodern.shaman
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: intelligentlife]
    #17061912 - 10/19/12 02:34 PM (11 years, 3 months ago)

Most are easy to find in the US however I am not sure about Europe.

I doubt this is an actual chimera but this is unique in the fact that my other grafts never exhibited this. I'll see if this continues or is shown in the new growth of the scion. This may just be damage from being cut and held with pressure. :shrug:
Scion appears to have an extra 'skin' and the union is strange.



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Invisiblemodern.shaman
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: modern.shaman]
    #17149112 - 11/02/12 04:48 PM (11 years, 2 months ago)

So I randomly stumbled upon chimera online.

Gymnocalycium mihanovichii variegata x Hylocereus


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Invisibledurian_2008
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks *DELETED* [Re: modern.shaman]
    #17150718 - 11/02/12 09:56 PM (11 years, 2 months ago)

Post deleted by durian_2008

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Invisiblepsi
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: durian_2008]
    #17151011 - 11/02/12 11:03 PM (11 years, 2 months ago)

Colchicine is pretty scary shit. I believe it can also be used to create a true breeding diploid plant from a haploid cell line from pollen or anther culture.

http://en.wikipedia.org/wiki/Doubled_haploidy


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Invisibledurian_2008
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks *DELETED* [Re: psi]
    #17151060 - 11/02/12 11:13 PM (11 years, 2 months ago)

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Invisiblepsi
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Re: Creating a Cactus Chimera's- A Reason For Experimenting With Different Stocks [Re: durian_2008]
    #17152000 - 11/03/12 06:39 AM (11 years, 2 months ago)

People used to always talk about wanting to use it to create polypoid weed, not sure if anything useful ever came of that. It's cool that you can just cultivate C. autumnale to make it, but I would not want to pursue it personally. I wonder if that species is immune to colchicine's effects or what.


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