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InvisibleCH HELL
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Sterile substrate vs pasteurized sub..........
    #17037330 - 10/15/12 06:58 PM (11 years, 4 months ago)



  I have been experimenting for several months now.  I started with side by side grows in myco bags(unicorn bags with .2um filters) with just coir, verm and gypsum.  Both sterile and pasteurized substrates did equally well and no contams.  The bags were all the same 2quarts of sub and then inoculated with one quart spawn.  The average yeild was 1.75 oz dry from each bag.

  Now my friends say I just got lucky with my sterile bags and they would have contamed if I didn't inoculate them in front of my flowhood.  Okay so the next batch of bags I did were all sterilized and inoculated without the help of my flow hood.  I only did the normal cleaning and spraying the air with lysol,  still zero contams.
  So now I am questioning myself and saying ahh this is just becuase it is a coir based substrate.  I really see no need to pastuerize coir sterilizing it is not going to change it.  The way coir is made into bricks it is cooked at a much higher temp than what we would use to sterilize.
  After these tests I decided to push my luck and go all out Manure, coir, straw, coffee, verm and gypsum.  These were brought to feild compacity and then two quarts got put into the same still filter patch bags and pressure cooked for 60 minutes.  I am not saying 60 minutes at 15psi is going to completely sterilize things but it isn't pasteurized either.
  Here is what the bag looked like before PCing.

  I did five bags like this becuase that is how many I could fit in my PC.  The next day when then bags had cooled(in the pc) they were inoculated with one quart spawn.  Two of them with PFRS and three with PE6.  Still zero contamination,  it can't be luck because I move in this house about 2 years ago and had major contamination problems when I got here. 
  I have now done approx 25 bags with this recipe with zero contams and have been averaging 2.5 oz dry from each bag,  that is 2.5 oz dry from 1 quart of spawn.:eek: My largest first flush was 3.1oz dry.
 

  So enough messing around with bags I have proven to myself this works lets try a Mono tub.  On the second of this month I sterilized 9 quarts of this mix(Manure, coir, straw, verm and gypsum) in three myco bags.  O the third of the month I added two quarts spawn to each bag,  I had intended to just shake them and put them in a Mono but I didn't have time that day.
So on the 4th of October the bags looked like this....

They had already started to recover,  so I don't know if this was a good thing or a bad thing  but I moved forward adding them to a Mono tub.



Bags added..



Mixed and leveled out,  my math was off for this tub and my substrate is an inch low:mad: nothing I can do about it now..


Anyone want to bet on how the tub is doing?

10-16



10-18 Well as I expected this tub got trich.  Believe I should have done a side by side to show that any open air tub will contaminate in this house.  If you look closely at the picture you will see the trich formed under the easy felt that was not clean and only taped on the top,  If I had the entire tub sealed I think it would have been fine,  but one small gap and boom trich city.



Feel coir and straw would be a great substrate that will perform well sterilized.  For now sterilizing is fine with or with out a flow hood in filter patch bags or controlled enviroment.


Edited by CH HELL (10/20/12 02:58 PM)


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Invisibleserotonin storm
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Re: Sterile substrate vs pasteurized sub.......... [Re: CH HELL]
    #17037367 - 10/15/12 07:04 PM (11 years, 4 months ago)

i'll bet it's doing just fine.


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InvisiblesporeRider
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Re: Sterile substrate vs pasteurized sub.......... [Re: serotonin storm]
    #17037426 - 10/15/12 07:11 PM (11 years, 4 months ago)

Don't worry to get heat for tryin new things to simplify your techniques :cheers: rock em my good man :smile:. My method for cubes seemed such taboo but I got more than satisfying results with minimal work. I kept doing what worked for me and gave many of my ghetto tek kits to friends who also saw the simplicity of success and great yields with no watering or fanning. Do what works and keep learning from mistakes-that's life man


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OfflineRogerRabbitM
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Re: Sterile substrate vs pasteurized sub.......... [Re: CH HELL]
    #17037455 - 10/15/12 07:15 PM (11 years, 4 months ago)

Quote:

CH HELL said:
Okay I do believe I will get slammed for this and maybe even lose my TC tag but I feel things need to be addressed.






:machinegun:

j/k.  Mold spores have a hard time germinating on coir, so whether it's pasteurized or sterilized makes little difference.  In addition, when you're colonizing in filter patch bags which keep the mold spores out, you can sterilize manure, straw, compost, etc., with no loss in performance.
RR


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OfflineTheDeathryder
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Re: Sterile substrate vs pasteurized sub.......... [Re: sporeRider]
    #17037471 - 10/15/12 07:17 PM (11 years, 4 months ago)

I'm interested to see how this turns out :smile:


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InvisibleCH HELL
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Re: Sterile substrate vs pasteurized sub.......... [Re: TheDeathryder]
    #17038006 - 10/15/12 08:27 PM (11 years, 4 months ago)

Quote:

RogerRabbit said:
Quote:

CH HELL said:
Okay I do believe I will get slammed for this and maybe even lose my TC tag but I feel things need to be addressed.






:machinegun:

j/k.  Mold spores have a hard time germinating on coir, so whether it's pasteurized or sterilized makes little difference.  In addition, when you're colonizing in filter patch bags which keep the mold spores out, you can sterilize manure, straw, compost, etc., with no loss in performance.
RR




:super:  Yes this is why 90% of edible grows are sterilized and the other 10% are not because the strain calls for endospores.

No bets on the Mono tub RR?

Quote:

sporeRider said:
Don't worry to get heat for tryin new things to simplify your techniques :cheers:




Thanks SR you know I don't worry about taking heat and if the TC tag goes it doesn't matter I will still be doing my thing.:hehehe:

Quote:

TheDeathryder said:
I'm interested to see how this turns out :smile:





I will open her up tomorrow and take some pictures.


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InvisibleTranscendingLife
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Re: Sterile substrate vs pasteurized sub.......... [Re: CH HELL]
    #17038265 - 10/15/12 08:54 PM (11 years, 4 months ago)

I'm here.
Already in the midst of trying this "experiment."


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Offlineiwannabemro
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Re: Sterile substrate vs pasteurized sub.......... [Re: TranscendingLife]
    #17038348 - 10/15/12 09:09 PM (11 years, 4 months ago)

I bet it comes out fine.  I sterilized my first bulk grow not knowing any better, cpoo coir. Came out fine.


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Invisibleprismism
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Re: Sterile substrate vs pasteurized sub.......... [Re: CH HELL]
    #17038451 - 10/15/12 09:25 PM (11 years, 4 months ago)

Break the mold.


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InvisibleCH HELL
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Re: Sterile substrate vs pasteurized sub.......... [Re: prismism]
    #17038487 - 10/15/12 09:31 PM (11 years, 4 months ago)

Quote:

prismism said:
Break the mold.


:hehehe: :highfive:


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OfflineProfessorPinHead
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Re: Sterile substrate vs pasteurized sub.......... [Re: CH HELL]
    #17041092 - 10/16/12 10:27 AM (11 years, 4 months ago)

I've tried similar and had the same results.

I never did have the balls to say anything about it, because of the same reasoning you were tossing to the table.

I always thought it was because of the high spawn ratio, but I guess there is more to the story than meets the eye,

Neat observation & post CH!

-PP.>


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OfflineRogerRabbitM
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Re: Sterile substrate vs pasteurized sub.......... [Re: CH HELL]
    #17041514 - 10/16/12 11:57 AM (11 years, 4 months ago)

Quote:

CH HELL said:
I will get slammed for this and maybe even lose my TC tag




I should pull it just to fuck with ya. :laugh:
RR


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OfflineProfessorPinHead
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Re: Sterile substrate vs pasteurized sub.......... [Re: RogerRabbit]
    #17041542 - 10/16/12 12:03 PM (11 years, 4 months ago)

Quote:

RogerRabbit said:
Quote:

CH HELL said:
I will get slammed for this and maybe even lose my TC tag




I should pull it just to fuck with ya. :laugh:
RR



And here I was thinking I was the one being messed with,..... :hehehe:


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InvisibleCH HELL
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Re: Sterile substrate vs pasteurized sub.......... [Re: ProfessorPinHead]
    #17042203 - 10/16/12 02:07 PM (11 years, 4 months ago)

Well I was hoping to see some pins.  No pins but it is white and has a nice mushroom smell.



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OfflineProfessorPinHead
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Re: Sterile substrate vs pasteurized sub.......... [Re: CH HELL]
    #17042219 - 10/16/12 02:10 PM (11 years, 4 months ago)

Looks to be "on the verge" to me, :thumbup:


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InvisibleCH HELL
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Re: <span>Re: Ster<span id="dtx-highlighting-item" dtx-highlight-backgroundcolor="lime">i</span>le substrate vs pasteur<span id="dtx-highlighting-item" dtx-highlight-backgroundcolor="lime">i</span>zed sub..........</span&g [Re: ProfessorPinHead]
    #17042261 - 10/16/12 02:18 PM (11 years, 4 months ago)

Quote:

ProfessorPinHead said:
Looks to be "on the verge" to me, :thumbup:





It is very close.  It was 12 days I could have waited a couple more but I opened it so I removed the tape and put it into fruiting today.  I don't think I need to worry about it contaminating now but it would have been a better pin set if I had waited I am sure.

CH


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InvisibleCH HELL
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Re: Sterile sub [Re: RogerRabbit]
    #17042314 - 10/16/12 02:25 PM (11 years, 4 months ago)

Quote:

RogerRabbit said:
Quote:

CH HELL said:
I will get slammed for this and maybe even lose my TC tag




I should pull it just to fuck with ya. :laugh:
RR




:lmafo:
Be nice RR.  It is all in the name of science. :havesomescience:

CH


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