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SMAGE
Peppermint
Registered: 04/21/03
Posts: 127
Loc: In your head
Last seen: 19 years, 3 months
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Need help making a syringe last longer with honey, quick
#1699781 - 07/09/03 01:31 PM (20 years, 8 months ago) |
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Here's the deal: i am down to 5ml of spore solution, i can't afford any more at the moment so i have been looking into useing the honey tek to make a liquid mycellium culture. after searching for honey tek in the posts i've come to this plan fill a quart jar with water and honey (i think a quart is about a liter)
add two marbles to help with breaking up mycellium
poke a hole in the lid and cover with tape to use for innoculation and sucking up mycellium.
pressure cook for 10 min
take out and tighten lid
innoculate with spores
incubate at 80-83 degreese until colonized
suck up with syringe what you need and store rest in fridge
Now i have a few questions
1. How much honey do i add for a quart of water? (i've heard mixed opinions about this) 2. Do i need to add any h202? 3. how long will it save in the fridge
thanks in advace for your help, after i do this i might make a tek with pics out of it
-------------------- I dont know what your problem is, but I'll bet its hard to pronounce.
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SMAGE
Peppermint
Registered: 04/21/03
Posts: 127
Loc: In your head
Last seen: 19 years, 3 months
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Re: Need help making a syringe last longer with honey, quick [Re: SMAGE]
#1699817 - 07/09/03 01:44 PM (20 years, 8 months ago) |
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oh and any other pointers are welcome
-------------------- I dont know what your problem is, but I'll bet its hard to pronounce.
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Psilocybin_monkey
Shroomer
Registered: 06/19/03
Posts: 1,340
Loc: Dragon's Den
Last seen: 12 years, 5 months
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Re: Need help making a syringe last longer with honey, quick [Re: SMAGE]
#1699866 - 07/09/03 02:04 PM (20 years, 8 months ago) |
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4% honey per 96% water. it last about a month in the fridge before all the sugars are used up. and no you don't need any hydro peroxide but you do need to pc it for 20 mins @ 15psi if you pc it for any longer the sugars will carmelize. other then that you got have it all to a tee
-------------------- Welcome to my world!
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SMAGE
Peppermint
Registered: 04/21/03
Posts: 127
Loc: In your head
Last seen: 19 years, 3 months
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Re: Need help making a syringe last longer with honey, quick [Re: Psilocybin_monkey]
#1699926 - 07/09/03 02:26 PM (20 years, 8 months ago) |
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are the %'s by volume?
-------------------- I dont know what your problem is, but I'll bet its hard to pronounce.
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thrice
Stranger
Registered: 12/27/02
Posts: 164
Last seen: 18 years, 7 months
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Re: Need help making a syringe last longer with honey, quick [Re: SMAGE]
#1700202 - 07/09/03 04:15 PM (20 years, 8 months ago) |
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How long does colonization of the honey take?
-------------------- "I've got high friends in places".
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Psilocybin_monkey
Shroomer
Registered: 06/19/03
Posts: 1,340
Loc: Dragon's Den
Last seen: 12 years, 5 months
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Re: Need help making a syringe last longer with honey, quick [Re: thrice]
#1700236 - 07/09/03 04:30 PM (20 years, 8 months ago) |
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yes they are by volume for example a 1/2 pint = 250ml so it will be 10ml of honey and 240ml of water.Well i use honey crystals and it take about 5-7 weeks to colonize and most times more i get germation withing the frist week always and I can see mycelium growing in about 1-2 weeks but it may be faster to use real honey i don't use it because i like to use powders over liquids
-------------------- Welcome to my world!
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