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OfflineSMAGE
Peppermint

Registered: 04/21/03
Posts: 127
Loc: In your head
Last seen: 12 years, 9 months
Need help making a syringe last longer with honey, quick
    #1699781 - 07/09/03 03:31 PM (14 years, 3 months ago)

Here's the deal: i am down to 5ml of spore solution, i can't afford any more at the moment so i have been looking into useing the honey tek to make a liquid mycellium culture. after searching for honey tek in the posts i've come to this plan
fill a quart jar with water and honey (i think a quart is about a liter)

add two marbles to help with breaking up mycellium

poke a hole in the lid and cover with tape to use for innoculation and sucking up mycellium.

pressure cook for 10 min

take out and tighten lid

innoculate with spores

incubate at 80-83 degreese until colonized

suck up with syringe what you need and store rest in fridge

Now i have a few questions

1. How much honey do i add for a quart of water? (i've heard mixed opinions about this)
2. Do i need to add any h202?
3. how long will it save in the fridge

thanks in advace for your help, after i do this i might make a tek with pics out of it


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I dont know what your problem is, but I'll bet its hard to pronounce.


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OfflineSMAGE
Peppermint

Registered: 04/21/03
Posts: 127
Loc: In your head
Last seen: 12 years, 9 months
Re: Need help making a syringe last longer with honey, quick [Re: SMAGE]
    #1699817 - 07/09/03 03:44 PM (14 years, 3 months ago)

oh and any other pointers are welcome


--------------------
I dont know what your problem is, but I'll bet its hard to pronounce.


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OfflinePsilocybin_monkey
Shroomer

Registered: 06/19/03
Posts: 1,340
Loc: Dragon's Den
Last seen: 6 years, 8 days
Re: Need help making a syringe last longer with honey, quick [Re: SMAGE]
    #1699866 - 07/09/03 04:04 PM (14 years, 3 months ago)

4% honey per 96% water.
it last about a month in the fridge before all the sugars are used up.
and no you don't need any hydro peroxide but you do need to pc it for 20 mins @ 15psi if you pc it for any longer the sugars will carmelize.
other then that you got have it all to a tee


--------------------
Welcome to my world!



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OfflineSMAGE
Peppermint

Registered: 04/21/03
Posts: 127
Loc: In your head
Last seen: 12 years, 9 months
Re: Need help making a syringe last longer with honey, quick [Re: Psilocybin_monkey]
    #1699926 - 07/09/03 04:26 PM (14 years, 3 months ago)

are the %'s by volume?


--------------------
I dont know what your problem is, but I'll bet its hard to pronounce.


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Offlinethrice
Stranger

Registered: 12/27/02
Posts: 164
Last seen: 12 years, 2 months
Re: Need help making a syringe last longer with honey, quick [Re: SMAGE]
    #1700202 - 07/09/03 06:15 PM (14 years, 3 months ago)

How long does colonization of the honey take?


--------------------
"I've got high friends in places".


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OfflinePsilocybin_monkey
Shroomer

Registered: 06/19/03
Posts: 1,340
Loc: Dragon's Den
Last seen: 6 years, 8 days
Re: Need help making a syringe last longer with honey, quick [Re: thrice]
    #1700236 - 07/09/03 06:30 PM (14 years, 3 months ago)

yes they are by volume for example a 1/2 pint = 250ml so it will be 10ml of honey and 240ml of water.Well i use honey crystals and it take about 5-7 weeks to colonize and most times more i get germation withing the frist week always and I can see mycelium growing in about 1-2 weeks but it may be faster to use real honey i don't use it because i like to use powders over liquids


--------------------
Welcome to my world!



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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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