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Offlinescottshrooms
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Registered: 04/29/03
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Spore printing question
    #1698278 - 07/09/03 02:49 AM (13 years, 4 months ago)

Okay, so i was down to my last syringe (store bought) and i wanted to try making my own from a big fresh cap using psylocybers printing tek. Im pretty sure that was the tek i used, sorry im not positive it was that specific tek, but anyway... I scraped the spore print into sterile syringes of water. I then let them sit in the fridge for around 24 hours before i innoculated. I inoculated 7 Ball jars and marked the two from the store bought ones. I incubated for almost two weeks now around 85 degrees in the dark. one already went into the fruiting chamber, one stopped growth except one little 1/2 inch spot on the bottom( which i flipped upside down for a little air exchange), and the others have maybe one or two little spots on them and they wont grow anymore either. No signs of contams just serious growth halt. Did i not prep the spore prints right or sterile enough? Is there any chance of saving them or fixing them?


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InvisibleMycoCakeEater
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Re: Spore printing question [Re: scottshrooms]
    #1698297 - 07/09/03 02:56 AM (13 years, 4 months ago)

That sounds like a problem of low-moisture. Hmmm.


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InvisibleATWAR
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Re: Spore printing question [Re: scottshrooms]
    #1698309 - 07/09/03 03:00 AM (13 years, 4 months ago)

If I were to take a guess I would say you may have a bacterial contamination in your syringe, commonly caused by improper sterilization techniques. There is no way you can save the syringes, but keep an eye on the jars. You should see some sort of discharge or discolorations appear if they are contaminated. Or, you may just see the growth stall and never advance (or grow extremely slowly, I have seen bacterial contamination be taken over by healthy mycelium).

All you can do is watch them, and clean them out and start over if you have a doubt.


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Invisiblegeorge castanzaM
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Re: Spore printing question [Re: ATWAR]
    #1698359 - 07/09/03 03:15 AM (13 years, 4 months ago)

kramer says atwar is right on the money on this one.
unless the jars are dry ,you can bet it's a bacterial.
if a jar dose not fully colonize at a somewhat steady rate of growth
it is not worth messing with trying to save or what not .
it's much easier to start over with fresh syringes and substrate
b/c you waste more time and still get nothing
kramer says to toss any good myc into an outdoor experiment and start fresh with wbs and grow eq or ks next time


at least thats what kramer says


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pftek said:Impossible to clean everything right now. Maybe in a week.





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Offlinescottshrooms
Explorer ofAnother Realm
Registered: 04/29/03
Posts: 27
Last seen: 13 years, 4 months
Re: Spore printing question [Re: george castanza]
    #1698889 - 07/09/03 07:29 AM (13 years, 4 months ago)

okay, at a closer look it seems as though the jars do indeed look drier than the two substrate jars that did colonize...and fast too! Is there any way to add more water? Can i dunk them or is it too risky of contams because they aren't colonized? I need any and all legitimate help, i dont want to hafta throw out five jars!


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OfflineMAIA
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Re: Spore printing question [Re: scottshrooms]
    #1698892 - 07/09/03 07:34 AM (13 years, 4 months ago)

You can inject some sterile water with a syringe but don't expect much.

MAIA


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