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InvisibleMr Flipper
Registered: 09/30/12
Posts: 75
Problems With Agaricus Bisporus Culture Growth *DELETED*
    #16936928 - 09/30/12 09:26 AM (11 years, 3 months ago)

Post deleted by Mr Flipper

Reason for deletion: deleted account



Edited by Mr Flipper (10/07/12 05:13 PM)


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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: Problems With Agaricus Bisporus Culture Growth [Re: Mr Flipper]
    #16938462 - 09/30/12 01:46 PM (11 years, 3 months ago)

Quote:

Mr Flipper said:
Method of starting culture: live tissue samples of agaricus bisporus, harvested fresh from a local mushroom farm's crop less than six hours prior (gave me a handful free).




There is your problem right there.  Try dropping some clean gill tissue on the agar instead and see what happens. Large button spawn producers expand their grain like crazy leaving spawn only suitable for growing mushrooms and not subculturing.

Lipa


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Invisiblesparkle
Farmer
Female User Gallery


Registered: 07/28/11
Posts: 1,133
Loc: Philippines Flag
Re: Problems With Agaricus Bisporus Culture Growth [Re: lipa]
    #16939512 - 09/30/12 04:30 PM (11 years, 3 months ago)

Some of the plates look alive but it's too soon to say either way. Sometimes even if it browns it might still live. Activated charcoal in the media helps lessen browning.
I cloned a store-bought portabella before and it raced through, even plain straw but I couldn't fruit it in my temps.
5-7 days after cloning I think


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Offlineprimrose
Stranger
Female

Registered: 03/01/09
Posts: 69
Loc: USA
Last seen: 8 years, 3 months
Re: Problems With Agaricus Bisporus Culture Growth [Re: sparkle]
    #16940148 - 09/30/12 06:12 PM (11 years, 3 months ago)

The best thing you can do for making Agaricus bisporus spawn is to read the article publised by Dr. B.B. Stroller.  It is "Some Practical Aspects of Making Mushroom Spawn".  It is available at the University of Penn. State Library in the Kneebone collection.

It is a step by step article.  However, in my humble spawn making the following is what I do.

You should snap the stem off cap and use the cells from the small circle on the cap that held the stem.  Only dig in about 1/8" deep.  Those cells are rapid growing and grow very well on plates.

Hope this helps you.


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Offlinenasser
shroomer
Male User Gallery

Registered: 08/27/11
Posts: 29
Loc: jordan Flag
Last seen: 8 years, 9 months
Re: Problems With Agaricus Bisporus Culture Growth [Re: primrose]
    #18970199 - 10/12/13 10:17 PM (10 years, 3 months ago)

hey how r u
i tried to google search the article "Stoller BB. Some practical aspects of making mushroom spawn " with no success do u have a copy
thank u


--------------------
Life is no more than a piss of shit
why caring about it


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