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Offlinepsilo9com
Koh Koh Gadget

Registered: 05/20/03
Posts: 267
Last seen: 20 years, 4 months
How do you inoculate a quart sized grain jar?
    #1680178 - 07/02/03 11:32 AM (20 years, 6 months ago)

AFOAF just finished up his first flush of shrooms, and isn't a big fan of cloning (tried it, just won't work for him).

If he fills up some grain jars 2/3 full with wbs, could he use spores to inoculate em? I know it won't be as fruitful a substrain as you can get if you clone, but i don't think I care much. I think once one jar is done is G2G time.


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 14 years, 1 month
Re: How do you inoculate a quart sized grain jar? [Re: psilo9com]
    #1680235 - 07/02/03 11:50 AM (20 years, 6 months ago)

Check out these results with WBS and honey tek, 2 days after innoculation. Honey tek is by far the way to go (or some form of liquid mycelium). Shake the jars right after PCing and then right after innoculation works fantastic!



Spores take much longer, almost a week and a half to get there. But they work.


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


Edited by kukusz (07/02/03 11:51 AM)


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Offlineresin
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Registered: 06/12/02
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Re: How do you inoculate a quart sized grain jar? [Re: kukusz]
    #1680274 - 07/02/03 12:05 PM (20 years, 6 months ago)

Yea you can use spores. Just use a syringe and squirt 3-4cc's solution around the rim of the jar. G2G Is the way to go


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 14 years, 1 month
Re: How do you inoculate a quart sized grain jar? [Re: resin]
    #1680310 - 07/02/03 12:20 PM (20 years, 6 months ago)

Hey resin, how do you get G2G to spread out as well as liquid mycelium culture? Even shaking the shit out of the jar only results in a small number of innoculation points, no? AFOAF had these popcorn jars, and no matter how much he shook, they didn't colonize, while a honey tek innoculation to the same batch of popcorn tore through it in four days.


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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Invisibleangryshroom
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Registered: 12/18/01
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Re: How do you inoculate a quart sized grain jar? [Re: kukusz]
    #1680560 - 07/02/03 02:25 PM (20 years, 6 months ago)

Inoculate with spores/liquid mycelium/mycelium culture, shake and wait a few days. Shake again, and wait another few days. You'll get near 100% colonization by then.

Fastest will be with a culture though, rhizomorphic growth tears through substrate like butta.


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Offlinelemunhed
The hustler'shustler

Registered: 05/12/03
Posts: 775
Loc: Ur moms house
Last seen: 18 years, 6 months
Re: How do you inoculate a quart sized grain jar? [Re: angryshroom]
    #1680743 - 07/02/03 03:33 PM (20 years, 6 months ago)

Yeah it does. I have a friend who has been doing popcorn, it takes him about 2 weeks to 100% colonization going from strait spores. That's still a shitload faster than PF cakes, and is pretty awesome in my friends opinion.


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You're pompeius, aren't you? I'll make a pompeian of you unless you hold your toungue!

-Tiberius Claudius Nero


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InvisibleOdin 13
Excitable Boy

Registered: 01/07/00
Posts: 62
Re: How do you inoculate a quart sized grain jar? [Re: lemunhed]
    #1680890 - 07/02/03 04:16 PM (20 years, 6 months ago)

One way to make the G2G a little more efficient, is to dump the first grain jar (the master) into a gallon baggie and crumble it really fine. Allow it to sit a few days to recover, and then proceed to inoculate the other jars. This seems to work fine for me. The smaller grains promote growth from more sites, and the recovery time "wakes up" the spawn.


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Offlinepsilo9com
Koh Koh Gadget

Registered: 05/20/03
Posts: 267
Last seen: 20 years, 4 months
Re: How do you inoculate a quart sized grain jar? [Re: Odin 13]
    #1684453 - 07/03/03 04:12 PM (20 years, 6 months ago)

Ok, so here's the plan.

I want to get spores, waste maybe 6cc per jar, do 2 jars, and then when those babies colonize pull a g2g and 0wn up those quarts.

I have some pf-cakes in my terrarium right now, but they've been spored all over. They still produce mushrooms though.Could I take this cake, crumble it in a baggie, and then do G2G? Would the oven tek be good to use for that?


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OfflineZodiacz
Simply Amazing
Registered: 10/02/02
Posts: 76
Loc: Over Here Now
Last seen: 11 years, 10 months
Re: How do you inoculate a quart sized grain jar? [Re: psilo9com]
    #1684931 - 07/03/03 08:36 PM (20 years, 6 months ago)

I would not use a crumbled up PF Cake for G2G unless just to say what da phuk. Too much possibility to contaminate fresh grain. Why not just start off with a fresh quart of grain or popcorn and inject those ??? :mushroom2: :mushroom2: :mushroom2: 


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YUM YUM EAT EM UP !


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Offlinepsilo9com
Koh Koh Gadget

Registered: 05/20/03
Posts: 267
Last seen: 20 years, 4 months
Re: How do you inoculate a quart sized grain jar? [Re: Zodiacz]
    #1693362 - 07/07/03 02:18 PM (20 years, 6 months ago)

what about the inside of a stem? or the inside of the cake?

afoaf has all these nice pretty qts waiting to be used.


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