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InvisibleinskiM
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Fruiting capabilities of clone isolates?
    #16923808 - 09/27/12 10:15 PM (11 years, 4 months ago)

As many of you may know, it's possible to isolate more than one pure strain from a single clone, has anybody run tests on the fruiting capabilities of all of the distinct cultures that can be acquired from tissue culture made from a single fruit body?


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Invisiblenotanumber
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Re: Fruiting capabilities of clone isolates? [Re: inski]
    #16923838 - 09/27/12 10:19 PM (11 years, 4 months ago)

wow. I didn't know that! genetically distinct isolates?


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InvisibleinskiM
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Re: Fruiting capabilities of clone isolates? [Re: notanumber]
    #16923876 - 09/27/12 10:25 PM (11 years, 4 months ago)

Well, genetically I'm not sure but when cloning mushrooms it can be common to see more than one distinct sector, at least, macroscopically different, I would like to know if all of these distinct cultures from a single fruit body are equally capable of producing fruit bodies and if so do the fruit bodies show much variation in macroscopic form?

There must be someone who has run tests on this!


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OfflineRogerRabbitM
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Re: Fruiting capabilities of clone isolates? [Re: inski]
    #16925097 - 09/28/12 04:39 AM (11 years, 3 months ago)

Yes, I've fruited the individual isolates and in my limited experiments, none fruited as well as the composite.  If you're wanting to isolate strains, start from spores.
RR


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Offlinetroncotron
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Re: Fruiting capabilities of clone isolates? [Re: RogerRabbit]
    #16927856 - 09/28/12 04:20 PM (11 years, 3 months ago)

Some years ago I cloned an albino fruit that appeared in a regular flush of P.cubensis thai. After isolating six sectors, they were fruited separately (hoping an albino flush)and the result was six flushes with similar characteristics (none of them albino).



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InvisibleinskiM
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Re: Fruiting capabilities of clone isolates? [Re: troncotron]
    #16928063 - 09/28/12 04:56 PM (11 years, 3 months ago)

Quote:

RogerRabbit said:
Yes, I've fruited the individual isolates and in my limited experiments, none fruited as well as the composite.  If you're wanting to isolate strains, start from spores.
RR



Thank you, that is what I was looking for.
The problem with my current experiment is that the species I'm working with just doesn't produce many spores and any attempts at acquiring prints and starting cultures has failed, I have literally tried to create hundreds of prints from this species with no success.
Under the microscope it is evident that it just doesn't have a good spore production, they are there but in very small numbers.
I decided that the best way to gain viable fruiting strains would be through tissue culture so I finally isolated some pure strains after many experiments with different agar recipes, some of the cultures were started from tissue excised from the stipe and I also placed a few small fresh gill fragments on agar, the gill tissue grew but also some of the few spores present germinated so I have some cultures that are pure clones and some that are pure strains isolated from the multispore germination.
The clones from the stipe tissue show about three different sectors, two are nice linear-slightly rhizomorphic mycelium and the other is extremely slow growing and very cottony so my pure isolates were made from the linear-slightly rhizomorphic sectors, I'll make some isolates of the cottony sectors and run experiments with mixtures of the different strains, I guess time will tell!

Quote:

troncotron said:
Some years ago I cloned an albino fruit that appeared in a regular flush of P.cubensis thai. After isolating six sectors, they were fruited separately (hoping an albino flush)and the result was six flushes with similar characteristics (none of them albino).





That is very interesting, do you or anyone else have any theories as to why none of your cloned cultures produced albino fruit bodies, is it possible that environmental or other factors could make some fruit bodies look albino?


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Offlinetroncotron
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Re: Fruiting capabilities of clone isolates? [Re: inski] * 1
    #16930945 - 09/29/12 04:22 AM (11 years, 3 months ago)

Mushroom pigmentation is genetically controlled. The locus involved in this process has been mapped in Agaricus and several QTLs controlling it have been found in P.ostreatus. Genes can be activated/repressed under different environmental conditions producing variations in phenotypic characters. Thus, a transient inactivation of the gene/genes controlling pigmentation would lead to albino fruits that wouldn´t pass this characteristic to their progeny. Rare, but feasible.
Anyway,  the albino fruit appeared in a flush stressed due to very low Hr.

http://www.ncbi.nlm.nih.gov/pubmed/9578631
http://www.ncbi.nlm.nih.gov/pubmed/22730765


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InvisibleinskiM
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Re: Fruiting capabilities of clone isolates? [Re: troncotron]
    #16933495 - 09/29/12 04:29 PM (11 years, 3 months ago)

Ok, interesting, so it is in the genes but can be present or not due to environmental conditions!

Did you save your cloned cultures?, you could try playing with the fruiting conditions in an attempt to get that albino gene to show itself again!


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InvisibleMush 4 Brains
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Re: Fruiting capabilities of clone isolates? [Re: troncotron]
    #16993374 - 10/08/12 07:21 PM (11 years, 3 months ago)

Quote:

inski said:
As many of you may know, it's possible to isolate more than one pure strain from a single clone, has anybody run tests on the fruiting capabilities of all of the distinct cultures that can be acquired from tissue culture made from a single fruit body?



I currently am. http://www.shroomery.org/forums/showflat.php?Cat=0&Number=16990644&page=0&vc=1#16990644

Quote:

troncotron said:
Some years ago I cloned an albino fruit that appeared in a regular flush of P.cubensis thai. After isolating six sectors, they were fruited separately (hoping an albino flush)and the result was six flushes with similar characteristics (none of them albino).





Very interesting, good work.


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