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Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 24 days
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Oudemansiella canarii or platensis?
#16902587 - 09/24/12 01:59 PM (11 years, 4 months ago) |
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This is just a starter thread for an ID, with detailed pictures to follow. I've succeeded in cultivating some Oudemansiella from a spore print supplied by SporesAndSpores. See THIS POST.
Bloodworm did some work on a specimen of this mushroom HERE.
I isolated a dozen pure strains from the print. I grew out all twelve in mini filter patch bags, and got some strains to pin:

Here is the one I have my hopes on.
 It is 30mm in diameter right now. I'm guessing that the cap will fully open tomorrow or the following day.
In the meantime, I've been studying the paper Three type specimens designated in Oudemansiella.
After reading it a few times and staring at all the figures, it seems to me that the most clearly distinguishing feature between O. canarii and O. platensis is the pilepellis. See figures 3 and 13. Yesterday, I practiced cutting scalps from the pellis of a random Pleurotus. My understanding is that this kind of section is the right one for showing details of the pellis cells.
As soon as I have some more pictures, I'll post them on this thread. I'd like advice on what to photograph, both macroscopically and through the microscope. Also suggestions on staining (I have all the standard ones). I'm hoping that the considerable ID expertise in this forum will give a consensus species identification!
-------------------- Liberté, égalité, humidité.
Edited by Terry M (09/24/12 02:09 PM)
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Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 24 days
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Re: Oudemansiella canarii or platensis? [Re: Terry M]
#16909481 - 09/25/12 03:55 PM (11 years, 4 months ago) |
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I think we have a winner ....
O. platensis!
First of all, here's what the whole mushroom looks like.
 Pileus is 67 mm in diameter. Stipe is 52 mm in length.
I was able to cut some good scalps from the pileal patch/flakes. Here are the terminal cells.
 160X
  400X 1 division = 2.5 μm
From the Petersen et al. paper I cited in the first post, here are drawings and descriptions of the corresponding cells.
 O. canarii
"Anatomy of scattered flecks on pileus surface (Fig. 2): typical slender, cylindrical pileipellis hyphae (without clamp connections) converge and gather into congested patches of erumpent hyphal chains in which terminal cells become increasingly inflated, eventually shorter and more broadly fusoid than the slender hyphae from which they arise. Terminal cells 12-55 × 12-17 μm, firm- to thick-walled (wall never more than 1 μm thick); contents homogeneous, hyaline singly, slightly more pigmented terminally. “H“ connections and branched hyphae numerous."
 O. platensis
"Scattered patches or warts on pileus surface composed of a polycystoderm (Fig. 17), the proximal cells of which are relatively narrow and long (45-86 × 8-10 μm) becoming more and more inflated until terminal cells are subglobose to sphaeropedunculate (29-48 × 22-44 μm), thin- to thick-walled (wall never more than 0.5 μm thick), with septa appearing double; clamp connections absent; contents homogeneous, hyaline."
Also note that the wart-like projections are visible if you zoom in on the first picture I posted. These are from where I cut the sections.
If anyone disagrees with my ID, please please post on this thread. If I need any more substantiating data now, when I have an absolutely fresh specimen, is the time to speak up!
-------------------- Liberté, égalité, humidité.
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suchen
Once and Future Noob



Registered: 06/28/11
Posts: 8,841
Loc: Shangri-la
Last seen: 3 years, 2 months
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Re: Oudemansiella canarii or platensis? [Re: Terry M]
#16909576 - 09/25/12 04:12 PM (11 years, 4 months ago) |
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Great work! I remember thinking when bloodworm originally posted that it was O. platensis based on the macromorphology and the Petersen et al paper.
-------------------- Rod Tulloss said: The bulb is the bulb. The volva is the volva. They have a very long term realtionship, but they’re “just friends.”
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Lennybernadino
Amazon grower


Registered: 09/16/09
Posts: 770
Loc: Iquitos, Peru
Last seen: 14 days, 15 hours
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Re: Oudemansiella canarii or platensis? [Re: suchen]
#16909669 - 09/25/12 04:33 PM (11 years, 4 months ago) |
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Platensis, I know Canaari well
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