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OfflineWMass
Trip Loaner
Registered: 06/07/03
Posts: 13
Loc: Massachusetts
Last seen: 21 years, 5 months
A shot inthe dark experiment
    #1688582 - 07/05/03 06:30 PM (21 years, 6 months ago)

Okay a guy I know innoculated his jars two weeks ago and there was no growth. He suspected he did'nt use enough water and BRF in the mix. The jars looked extremly dry so he took a jar and dumped the contents in a bowl. The substrate was extremly dry and was burnt on the inside (he used microwave sterilization)he knew all was probably lost. So just to see he dumped all the substrate in a bowl.He added water and BRF and rejared. He is expecting contams but wants to see if any mycelum forms. Hopefuly the spores are still active. He really is'nt expecting much but its an interesting experiment. What do ya think?

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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: A shot inthe dark experiment [Re: WMass]
    #1689129 - 07/05/03 11:03 PM (21 years, 6 months ago)

Not to say there isn't a chance in hell, but it's almost impossible to colonize/fruit a substrate that is not sterile (i.e. has been exposed to the air after sterilizing.)

IMO, I'd buy a pressure cooker, or at least boil the jars according to a tek. I am not an advocate of microwave sterilization.

Just my opinion,

--
Micro


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OfflineWMass
Trip Loaner
Registered: 06/07/03
Posts: 13
Loc: Massachusetts
Last seen: 21 years, 5 months
Re: A shot inthe dark experiment [Re: micro]
    #1689647 - 07/06/03 06:18 AM (21 years, 6 months ago)

I agree I heard he just made a second batch and this time boiled them.
So its just a waiting game.

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OfflineMAIA
World-BridgerKartikeya (DftS)
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Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
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Re: A shot inthe dark experiment [Re: WMass]
    #1690661 - 07/06/03 04:51 PM (21 years, 6 months ago)

Don't expect much, sometimes i do crazy stuff just for the kicks, success rate is very low.

MAIA


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