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OfflineMagician26
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rhizo's on a casing, to some seed in a jar.......
    #1688696 - 07/05/03 09:49 PM (13 years, 5 months ago)

would it be possible to start a jar of pc'ed wbs from some myc. from a casing?

what steps could i take to ensure success? what tools could i use and how do i prepare them? any comments and/or pearls of wisdom are welcome...


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Invisiblegeorge castanzaM
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Re: rhizo's on a casing, to some seed in a jar....... [Re: Magician26]
    #1688709 - 07/05/03 09:53 PM (13 years, 5 months ago)

use fresh spawn




that is kramers pearl


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Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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OfflineMagician26
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Re: rhizo's on a casing, to some seed in a jar....... [Re: george castanza]
    #1688738 - 07/05/03 10:06 PM (13 years, 5 months ago)

here is the deal....the substrate was cased after it failed to regenerate after a jar shaking, rather than try to spawn other jars with a possibly contaminated jar i opted to case it and hope for the best. as it is right now the myc. is showing strong rhizo. growth (even up the side of the rubbermaid). so i want to be able to innoc some jars as soon as i can.


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Invisiblegeorge castanzaM
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Re: rhizo's on a casing, to some seed in a jar....... [Re: Magician26]
    #1688745 - 07/05/03 10:07 PM (13 years, 5 months ago)

it is possible
but kramer says your begging for contams if you try it


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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OfflineMagician26
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Re: rhizo's on a casing, to some seed in a jar....... [Re: george castanza]
    #1688758 - 07/05/03 10:13 PM (13 years, 5 months ago)

you think i mihgt have some success with transfering the rhizos to a liquid culture jar??


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Invisiblegeorge castanzaM
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Re: rhizo's on a casing, to some seed in a jar....... [Re: Magician26]
    #1688765 - 07/05/03 10:17 PM (13 years, 5 months ago)

it is possible
but kramer says your begging for contams if you try it


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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OfflineMagician26
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Re: rhizo's on a casing, to some seed in a jar....... [Re: george castanza]
    #1688771 - 07/05/03 10:20 PM (13 years, 5 months ago)

Quote:

george castanza said:
it is possible
but kramer says your begging for contams if you try it





okay now that, that has been established.

has anyone attempted anything like this? i know i'm not giving the post enough time (me and george are probably the only ones watching this post on the edge of our seats...)

but any advice or help is welcome..and george thanks for the imput...


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Invisiblegeorge castanzaM
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Re: rhizo's on a casing, to some seed in a jar....... [Re: Magician26]
    #1688806 - 07/05/03 10:32 PM (13 years, 5 months ago)

sorry i couldn't be more helpful
kramer says maybe rinsing it with h2o2 might help?


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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OfflineMagician26
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Re: rhizo's on a casing, to some seed in a jar....... [Re: george castanza]
    #1688880 - 07/05/03 11:14 PM (13 years, 5 months ago)

i think i will try to spawn one jar from the rhizo adn see how that does before i try the other 7 of them. i figure i just need one good jar.....

now how to get that jar is the question........


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OfflineMAGICSNOOP
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Re: rhizo's on a casing, to some seed in a jar....... [Re: Magician26]
    #1688900 - 07/05/03 11:21 PM (13 years, 5 months ago)

yeah castanza is right but if it is done carefully this is what u do
take several pieces of nice looking myc. and transfer it to peroxide all in there own seperate containers so that way if one fails u got more to count on i dought all will fail out of lets say 5. take nice chunks then u do a transfer. it aint easy but possable


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InvisibleSorted
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Re: rhizo's on a casing, to some seed in a jar....... [Re: Magician26]
    #1689818 - 07/06/03 11:58 AM (13 years, 5 months ago)

I tried quite a similar thing a couple of days ago. There was lots of mycelium growing straight up from the casing layer on one of my tubs, so I picked one strand off with tweezers and dropped it in a jar of millet. Too early to tell if anything will come of it but looks like its grown a bit since it was put in..


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Anonymous

Re: rhizo's on a casing, to some seed in a jar....... [Re: Sorted]
    #1689826 - 07/06/03 12:04 PM (13 years, 5 months ago)

you could try adding h202 too the water you use for the wbs possibly..I have done this with brf and had some success


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OfflineMagician26
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Re: rhizo's on a casing, to some seed in a jar....... [Re: ]
    #1693974 - 07/07/03 07:59 PM (13 years, 5 months ago)

thank you all.... wish me luck.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Wild Bird Seed

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