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OfflineSev
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Registered: 06/06/03
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A thought on active contamination prevention
    #1687832 - 07/05/03 12:43 PM (14 years, 5 months ago)

Well, I've been up too late and it's gotten me to linking thought unto thought, and I came up with an idea.

So, we all know how pasteurization supposedly kills most of the 'harmful' contams but leaves some harmless crap to colonize the straw and keep the other contams out, right? Well, after remembering how there is some experimentation going on with forms of genetically engineered plaque that doesn't harm teeth but would outcompete the stuff that had, I had an idea...

...Has anyone tried innoculating a substrate with something that would work in this way? Flood the substrate with a competetive but compatable bacteria that would outcompete most of the contams until the mushrooms took over?


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"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

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Invisiblemicro
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Re: A thought on active contamination prevention [Re: Sev]
    #1687881 - 07/05/03 01:29 PM (14 years, 5 months ago)

I haven't, but there's been a lot done by proffesionals, most inoculate compost with bacteria like psudomonas putida (I think that's the one) and certain actinomycetes [edited] that thrive at really high temperatures (or put it in the casing layer.)

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Edited by micro (07/06/03 01:11 AM)


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Offlinezeronio
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Re: A thought on active contamination prevention [Re: Sev]
    #1688250 - 07/05/03 05:12 PM (14 years, 5 months ago)



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Invisiblewhodi
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Registered: 07/02/02
Posts: 92
Re: A thought on active contamination prevention [Re: zeronio]
    #1688353 - 07/05/03 06:15 PM (14 years, 5 months ago)

Doing any thing after pasteurization and before inoculating IMO seems a waste of time. Once mycelium colonizes the substrate there's far less chance other bacteria could contaminate..
Correct me if I'm wrong


Edited by whodi (07/05/03 06:16 PM)


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Invisiblemicro
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Re: A thought on active contamination prevention [Re: whodi]
    #1689089 - 07/06/03 12:40 AM (14 years, 5 months ago)

First of all -- I meant actinomycetes up there, not asco's.  Wrong order :wink:

Whodi -- are you talking about a substrate for jars/bags, or bulk?  I agree with bulk -- I just use straw, not even poo, and it works fine.  The actinos are usually a factor in composting when you're doing a really large amount (i.e. whole bales) at a time because they thrive at really high temperatures, and thus keep away a really good chunck of the bad stuff, but don't hinder what you're trying to grow.  Pseudomonas putita helps fruiting, too (higher yields with a lot of different species of mushrooms.)

But like I said -- I don't really see the point of going to all the hassle, unless maybe if you're a commercial grower.

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Micro


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Invisiblewhodi
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Registered: 07/02/02
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Re: A thought on active contamination prevention [Re: micro]
    #1691742 - 07/07/03 02:03 AM (14 years, 5 months ago)

Micro I was talking about jars and bags. I would think it?d be the same on bulk. Actually this thread (and the link) has opened my eyes to ways actinos can be used and not killed. I mean fermenting instead of pasteurization.
Thanks zeronio that link was great. Look like I have some experimenting to do.


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Invisiblemycofile
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Registered: 01/19/99
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Re: A thought on active contamination prevention [Re: whodi]
    #1692490 - 07/07/03 10:04 AM (14 years, 5 months ago)

I don't think it would work in bags and jars of spawn. Even the "good" micro-organisms will over run a nutrient rich substrate and spoil it.

For more info on psudomonas, try searching around the boards for vodka or acetone teks. Psudomonas grow naturally on alcohols secreted by mycelium, so simply letting a pasteurized medium (usually a casing) incubate after it has had acetone or vodka or something added will allow the psudomonas to thrive.


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