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Offlinepsylosymonreturns
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cardboard on agar? * 1
    #16855401 - 09/16/12 10:07 AM (11 years, 7 months ago)

So I tried searching about this idea but I couldnt really find what I was looking for . And I dont consider myself advanced in the slightest, but I cant ask questions in the cult forum because they are cube nazis.

Soooooooo,

I ve been dabbling in the arts of spore germination on agar finally . And have been having good success for a first timer.
So I streaked the plate with a wild Oregonian P ovoid. I followed what i read and transfered a thick leading edge of growth that looked the strongest and went on with my business.

So I didnt throw out that dish . And it never got any contams and had tons of thick growth so I didnt want to waste it. So i boiled some cardboard and in my glove box put a piece of soaked carboard rigt inside the dish with all those different strains fighting for dominance .

So its been a while and there is thick rhizomorphic strands of ovoid myc eating that cardboard from all sides. I tried it with the P bohemica plate i transfered off of and its looking good too . :shrug:


Obviously others have done this I imagine . Is it stupid to do shit like this ? Should I be chucking those first plates all the time ? And would these strands of myc attacking the cardboard be considered worthy of growing out ? I am planning on just adding that myc to egg cartons and boiled hardwood chips and making some ovoid bonsai pots and see what happens in the spring.


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Offlineblackdogs
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Re: cardboard on agar? [Re: psylosymonreturns]
    #16855419 - 09/16/12 10:14 AM (11 years, 7 months ago)

that's great.  i'm glad to hear the cardboard thing really works.

"Obviously others have done this I imagine . Is it stupid to do shit like this ? Should I be chucking those first plates all the time ? And would these strands of myc attacking the cardboard be considered worthy of growing out ? I am planning on just adding that myc to egg cartons and boiled hardwood chips and making some ovoid bonsai pots and see what happens in the spring. " 

no way.  i think it's a smart thing to do.  you'll be pioneering these techniques.  : )

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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: blackdogs]
    #16861509 - 09/17/12 10:01 AM (11 years, 7 months ago)

I just know, well from what ive read and ive read for many years before taking this stuff up. is that you dont want any excuse to invite contams into your scenario . but ive been diligent and also a bit lucky for a newb i think.

but the one benefit i can see from this is skipping the expensive grain . cardboard is free and wood chips are free so for the poor grower you can cut the cost of grain.

although grain gets colonized so damb fast its still worth it if you can afford it. :shrug:


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OnlineMacMerdin
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Re: cardboard on agar? [Re: psylosymonreturns]
    #16869514 - 09/18/12 03:28 PM (11 years, 7 months ago)

I don't see anything wrong with your idea.  In fact it inspired me to place a piece of cardboard (soaked under the faucet for a minute and then a quick dowsing of peroxide) onto a petri that I just spent taking isolates from.  I like experimenting.  :thumbup:

Keep us updated on your progress.

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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: MacMerdin]
    #16870260 - 09/18/12 05:48 PM (11 years, 7 months ago)

cool man!! :smile:  what species are you trying this with ?


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OnlineMacMerdin
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Re: cardboard on agar? [Re: psylosymonreturns]
    #16870413 - 09/18/12 06:22 PM (11 years, 7 months ago)

For the experiment:  ovoids.

This petri had three sectors on it so I'd imagine that I can get to a near isolate if it works?


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OfflineRiparianZoneJunky
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Re: cardboard on agar? [Re: MacMerdin]
    #16870982 - 09/18/12 07:55 PM (11 years, 7 months ago)

Nothing wrong with working with woodlovers!  Are you going to spawn from the cardboard to wood and then make an outdoor patch?


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: RiparianZoneJunky]
    #16871314 - 09/18/12 08:54 PM (11 years, 7 months ago)

ya thats the plan. i also like growing out woodlovers in bonsai pots as they are easy to keep watered with your other plants .


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Re: cardboard on agar? [Re: psylosymonreturns]
    #16871567 - 09/18/12 09:37 PM (11 years, 7 months ago)

Great idea, I'll have to try this.  I've got a patch of ovoids going that I transferred from stembutts.  I'm hoping it will fruit this spring, but I've heard they can take a year or two.  Good luck!:mushroom2:


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: RiparianZoneJunky]
    #16873265 - 09/19/12 07:50 AM (11 years, 7 months ago)

it really all depends how vigourous your myc is. a patch should fruit after a year if it didnt die and ive made bonsais in the spring that fruited by the fall. its a smaller space to colonize if you want fruits right away . its different when you put it in the ground though. ive lost more myc than ive had fruit in the ground.


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: psylosymonreturns]
    #16901654 - 09/24/12 10:29 AM (11 years, 7 months ago)

so i took the piece of cardboard off the ovoid dish the other day since it was almost fully covered. i mixxed it with more boiled cardboard. once those pieces are colonzed i will add wood chips.


so i nocced up some grain the other day with some blue oyster myc . there was some remaining myc along the edges so i put some boiled cardboard in that dish. well its already spreading across the cardboard. :thumbup: obviously oysters wouid work but hey gotta try.


Mods feel free to move this somewhere more apropriate since this is not really advanced stuff ! :smile:


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OfflineMateo
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Re: cardboard on agar? [Re: psylosymonreturns]
    #16919439 - 09/27/12 08:54 AM (11 years, 7 months ago)

I think there is much more benefit to grow woodlover mycelium on cardboard/eggkartons then the cost of grains.

When you want to increase mycelium mass of woodlovers for a grow you will want to colonize woodchips.
Now, the woodlover mycelium on the agarplate or grains won't colonize the woodchips as fast as mycelium grown on cardboard/eggkartons.
This is because the mycelium needs to "get used to" the new substrate wood.
This halt in growth happens all the time when you change to an unknown substrate for the mycelium.
The cardboard/eggkartons is made of woodmaterial and the mycelium can go right into colonization mode without halting when you mix with the woodchips later.

I have a couple of eggkartons that i pasturized, and in each "pit" i put a couple of colonized ryegrains (P.Azurescens).
The mycelium has now spread and have colonized about 50% of the eggkartons.
I keep the eggkartons in plastic bags to keep the moisture and have only opened the bags once for airexchange and a little misting.

So far i havent thought about what to do with the mycelium but i think i keep expanding the mycelium mass until spring and make a nice outdoor patch and maybee a small indoor experiment.

Woodlovers is so nice, i might try some others besides Azures also.


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: Mateo]
    #16924051 - 09/27/12 11:01 PM (11 years, 7 months ago)

ya its going good, cardboard is very resistant to contams and to me using the plate after youve already transfered sectors to grain , is well worth it. the oyster dish is thick just from the edges of the plate eating up the cardboard. i think it may be spreading better on the cardboard then rye grain.
the ovoids worked good and the P bohmeica is slow as always. but still no contams in any of my experimental dishes that ive transfered sections from.


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InvisibleinskiM
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Re: cardboard on agar? [Re: psylosymonreturns]
    #16924086 - 09/27/12 11:08 PM (11 years, 7 months ago)

What type of agar recipe did you initially use for your Psilocybe bohemica experiments?


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: inski]
    #16924360 - 09/28/12 12:01 AM (11 years, 7 months ago)

MEA.


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OfflineLennybernadino
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Re: cardboard on agar? [Re: psylosymonreturns]
    #16926317 - 09/28/12 11:31 AM (11 years, 7 months ago)

Why do you assume that others have obviously done this?  It is amazingly easy to do something no one else has ever done , we may be billions over time but what we have discovered and done is finite and the universe and posssabilities are infinite . It is a common myth that we believe that it is difficult to come up with a new ide or invention and if you believe that myth you will not try, if you do not it is easy .
    It seems like you came up with a good shortcut for isolating the fruiting aggressive strains from the fruitless time wsting strains.
    Crdboard would work with many diffirent species, I wonder wich species would not be cardboard compatible, and what could be used with them.
    jEr

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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: Lennybernadino]
    #16926836 - 09/28/12 01:08 PM (11 years, 7 months ago)

Well thanks mate, maybe it is a new idea. :smile: so far its working great and I will try it with every plate that i transfer off of.it will be interesting to see which species it will work for.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #16927939 - 09/28/12 04:32 PM (11 years, 7 months ago)

Quote:

psylosymonreturns said:
MEA.




Was the mycelium extremely slow on this medium?, if yes then you could try making a solution by boiling some aged sawdust from a similar type of tree to the ones that this species likes to fruit from in the wild, boil the sawdust for 20 minutes or so then strain the tea and use the water for your agar, I used this water with my usual MEA recipe but with about half the amount of malt extract on a species that was painfully slow on typical agar recipes and the mycelium almost doubled it's speed of growth!!


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Re: cardboard on agar? [Re: inski]
    #16929591 - 09/28/12 09:21 PM (11 years, 7 months ago)

Actually P. serbica var. bohemica is doing
pretty well on standard MEA in my experience.

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Re: cardboard on agar? [Re: stonesun]
    #16929642 - 09/28/12 09:32 PM (11 years, 7 months ago)

Ok, nice, I was conversing with a friend who is having problems with it being very slow, I have also been working with a species from Section Aztecorum which is very slow on MEA and PDA but when transfered to an agar medium as described above the mycelium almost doubled in speed of growth, I also suspect that the growth is slowed if a large amount of spores are germinated in close vicinity to one another, they all compete for nutrients, as you do transfers and get close to a pure culture the growth speeds up, good luck with your wood lover experiments:cool:


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Re: cardboard on agar? [Re: inski]
    #16930393 - 09/29/12 12:09 AM (11 years, 7 months ago)

I have found Serbica to be less than speedy/strong myself.

I will try this too.

I also found egg cartons to rock Mateo.  I like to open the ziplock
bags once in a while in front of a flowhood to freshen them up.

Take care,

JD


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: stonesun]
    #16931297 - 09/29/12 07:27 AM (11 years, 7 months ago)

Quote:

stonesun said:
Actually P. serbica var. bohemica is doing
pretty well on standard MEA in my experience.



Well they are not that slow now that I've isolated. But compared to my other projects I find P bohemica to be a bit slow. Like compared to P ovoid or P azure they are very slow and not as thick and rhizomorphic.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #16933475 - 09/29/12 04:24 PM (11 years, 7 months ago)

Yes, I believe the sublignicolous or secondary decomposers of wood are just naturally slow growing compared with their lignicolous cousins like P. ovoideocystidiata or P. azurescens making them a bit more difficult to cultivate!


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: inski]
    #16933997 - 09/29/12 06:02 PM (11 years, 7 months ago)

Hmm i didnt realize P bohemica was a secondary decomposer. it makes sense though now that ive seen the mycelium.

and you mention it is in the section Aztecorum? So like P baeocystis ? So are all the species in that section secondary decomposers?
so what substrate would you recommend ? i was going to try just straight wood chips like all other woodlovers . but maybe i should be using a substrate more suited for P caerulescens or P baeocystis.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #16934097 - 09/29/12 06:24 PM (11 years, 7 months ago)

Quote:

psylosymonreturns said:
Hmm i didnt realize P bohemica was a secondary decomposer. it makes sense though now that ive seen the mycelium.

and you mention it is in the section Aztecorum? So like P baeocystis ? So are all the species in that section secondary decomposers?
so what substrate would you recommend ? i was going to try just straight wood chips like all other woodlovers . but maybe i should be using a substrate more suited for P caerulescens or P baeocystis.




Oh, sorry, that was a misunderstanding, P. serbica is in section Cyanescens stirps Serbica, species in that group are generally secondary decomposers of wood, you should try fairly old wood and sawdust from trees that are similar to what the species fruits from in nature, probably conifers, it may colonise fresh wood but I doubt it will fruit from that for a few years, the wood needs to be old enough so that you can't smell the terpines anymore.

The species I am working with belongs in section Aztecorum, most species there are sublignicolous.


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Re: cardboard on agar? [Re: inski]
    #16934181 - 09/29/12 06:40 PM (11 years, 7 months ago)

I wonder if a spent shitake block might be useful for subs?


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Re: cardboard on agar? [Re: Javadog]
    #16934334 - 09/29/12 07:07 PM (11 years, 7 months ago)

Quote:

Javadog said:
I wonder if a spent shitake block might be useful for subs?



Hmm, not sure if it could colonise something that is already completely colonised by another fungus, maybe if you broke up the block, put it in jars and sterilised it again it could work, it's worth a try!


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Re: cardboard on agar? [Re: inski]
    #16934545 - 09/29/12 07:49 PM (11 years, 7 months ago)

Quote:

inski said:
Quote:

Javadog said:
I wonder if a spent shitake block might be useful for subs?



Hmm, not sure if it could colonise something that is already completely colonised by another fungus, maybe if you broke up the block, put it in jars and sterilised it again it could work, it's worth a try!




That is what I was thinking.  :wink:


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Offlinepsylosymonreturns
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Re: cardboard on agar? [Re: Javadog]
    #16935054 - 09/29/12 09:47 PM (11 years, 7 months ago)

i just collected some 2 -3 year old conifer chips that have had C lagopus, A praeocox and G luteofolius already rip through it. it looks to be free of any mycelium now as it dies back over the dry summer and i only collected from the top few inches. and once i sterilize i hope it will be a good substrate.

ive done unsterile work with other wood lovers, like P cyanescen and P azurescens and even SRA and i could cut a hazel nut or alder down and have it colonized in a few weeks. in my experience fresher the better. but honestly P bohemica is not as vigorous as even P cyanescen.P azurescen and SRA on the other hand are hands down the fastest most intense colonizers. P ovoids seems to be on the same level.


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Re: cardboard on agar? [Re: inski]
    #16935782 - 09/30/12 01:13 AM (11 years, 7 months ago)

Quote:

inski said:
Quote:

Javadog said:
I wonder if a spent shitake block might be useful for subs?



Hmm, not sure if it could colonise something that is already completely colonised by another fungus, maybe if you broke up the block, put it in jars and sterilised it again it could work, it's worth a try!





I think what Javadog is implying is that the mushroom species does not like substrates with the lignin still intact.  Maybe it relies on other fungi to "open it up" like Vollvariiella bombycina for example.

Lipa

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Re: cardboard on agar? [Re: lipa]
    #16936033 - 09/30/12 02:39 AM (11 years, 7 months ago)

Yes, I knew that was what he was implying but I thought he wanted to use the spent substrate without treating it, I believe it would need to be sterilised then inoculated with the sublignicolous species, it is a good idea and I hope he experiments and documents his findings.


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Re: cardboard on agar? [Re: inski]
    #16937382 - 09/30/12 11:12 AM (11 years, 7 months ago)

Oh I would give ole JD the benefit of the doubt. :thumbup: I know he knows he has to resterilize.

Ive read Stamets talking about using spent shiitake blocks resterilized for oyster growing. So its something thats done, not sure with these species were talking about though.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #16941404 - 09/30/12 09:18 PM (11 years, 7 months ago)

Yup.  Thank you both for the good word.

Indeed, given the learning I need to do, I will vary the approach,
by supplementing some of the blocks of semi-decomposed sawdust with
chopped straw and separately rice hull, and then a variety of other
standard supplements. (bran, coffee, gypsum, etc)

The other thing that I need to do is to setup the mini fridge that
I have mentioned elsewhere.  I have been percolating on the idea of
doing this since I wrecklessly purchased Azures (Hammond Flying Saucers from a sponsor)
as part of my very first purchases.  Boy, I was ahead of myself that
time, but I will need to push this to the next level.

This is an inspiring thread.

Thanks for sharing!

JD


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Re: cardboard on agar? [Re: Javadog]
    #16943404 - 10/01/12 08:54 AM (11 years, 7 months ago)

Quote:

Javadog said:
Yup.  Thank you both for the good word.

Indeed, given the learning I need to do, I will vary the approach,
by supplementing some of the blocks of semi-decomposed sawdust with
chopped straw and separately rice hull, and then a variety of other
standard supplements. (bran, coffee, gypsum, etc)

The other thing that I need to do is to setup the mini fridge that
I have mentioned elsewhere.  I have been percolating on the idea of
doing this since I wrecklessly purchased Azures (Hammond Flying Saucers from a sponsor)
as part of my very first purchases.  Boy, I was ahead of myself that
time, but I will need to push this to the next level.

This is an inspiring thread.

Thanks for sharing!

JD





ive been contemplating putting one of my azure bonsais in the garage fridge to see if a good cold shock can start an early flush.  i dont expect them to flush naturally until like november. last year they fruited outside until new years!

but an actual grow in the fridge would be really cool. i keep my eyes on craigslist to see if theres ever one of those glass door coolers you see in stores. growing azures inside could be a challenge but just growing the myc is VERY easy. it is ultra vigorous!!

not sure if you noticed mdma's indoor P cyanescen flush in the hunting forum! he had a box of colonized chips sitting above a cupboard in the kitchen and the fucking things started to fruit!!


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Re: cardboard on agar? [Re: psylosymonreturns]
    #16944035 - 10/01/12 10:52 AM (11 years, 7 months ago)

I did see that...amazing.

Never say impossible when it comes to fungi.  (remember the pumpkin guts grow ;0)

Take care,

JD


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Re: cardboard on agar? [Re: Javadog]
    #16944260 - 10/01/12 11:26 AM (11 years, 7 months ago)

yup hard to predict sometimes. :smile:


so back to the original topic i present some cardboard on agar.


Blue oyster . As you can see from the bottom shot i took sections out of the middle of the plate. leaving some thick edges. Ive read to be safe dont inoculate with the myc along the edge as its most likely to be closer to contams. but throw a picece of cardboard in there and it goes crazy since it is the most vigorous leading edge anyways and its a shame to waste it! :wink:



and i guess were calling this P serbica now.From Greece.


and heres a comparison of P serbica myc and P ovoid myc. although the ovoid is on its 3rd and serb is on its 2nd transfer. but there is clearly a difference in vigor and apearance. and just to be clear this is my very first time doing this stuff and my very first pours . my plates were on an angle and colected on the one side hence the side inoculations. :shrug: i'll do it better next time. :wink:



i keep my plates in ziplocs? does everyone do that?


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Edited by psylosymonreturns (10/01/12 07:02 PM)

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Re: cardboard on agar? [Re: psylosymonreturns]
    #16948878 - 10/01/12 09:50 PM (11 years, 7 months ago)

Lord, that ovoid mycelium is beautiful.  :0)

I do not bag my dishes....probably should, but I have soooo many
going at one time.  (and they are impossible to stack)

Good stuff.  Thanks for sharing.

JD


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Re: cardboard on agar? [Re: Javadog]
    #16949339 - 10/01/12 11:16 PM (11 years, 7 months ago)

Thanks man I need to Noc something up with that beauty soon!!

Today I used that oyster cardboard to Noc up some pasteurized cardboard and coffee grounds.So that will be the first grain skipper project. The myc was super thick on that cardboard so I'll give a update in a week.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17485707 - 01/02/13 10:56 AM (11 years, 4 months ago)

so just to update this thread and not leave yall hanging, here is the work in progress.

this is the carbaord i had put on the used P ovoid and P serbica agar plates. once the cardbaord was colonized i transfered pieces to boiled hardwood chips.

here it is. yes it works. :smile:
P ovoid

P serbica


to be honest my serbica project would have been screwed if i didnt do this as everything else fizzled out for me.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17486000 - 01/02/13 12:05 PM (11 years, 4 months ago)

Nice! Its really cool to see the simple natural tenchnique succede where the more advanced ones failed!

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Re: cardboard on agar? [Re: Lennybernadino]
    #17486016 - 01/02/13 12:11 PM (11 years, 4 months ago)

Excellent work Psy.

I appreciate your closing and sharing the matter.

I have found Serbica to be a tad finicky too.

Good luck,

JD


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Re: cardboard on agar? [Re: Javadog]
    #17486037 - 01/02/13 12:19 PM (11 years, 4 months ago)

Quote:

Lennybernadino said:
Nice! Its really cool to see the simple natural tenchnique succede where the more advanced ones failed!




i know eh! i will be doing this as back up now with all my projects! especially the slower less aggressive wood lovers like serbica, cyanfibs , stuntzii or caerulipes
Quote:

Javadog said:
Excellent work Psy.

I appreciate your closing and sharing the matter.

I have found Serbica to be a tad finicky too.

Good luck,

JD




thank you JD! :smile: it was a cool little project , i was glad to share!
and ya serbs are pretty finicky for sure eh! but once the mycelial mass is bigger it starts to wake up.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17537917 - 01/12/13 02:18 PM (11 years, 4 months ago)

Awesome man, I did something like that with cubensis.
I took my freinds failed jars ( he didnt listen to me, he innoc'd with no sterile procedures... in a 100 year old house :facepalm3:
I cloned out seven types of mold using cardboard, then used that to innoc pf jars, cardboard works great as a cheap hardy alternative to agar.
:nyan:


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Re: cardboard on agar? [Re: Joust]
    #17546746 - 01/14/13 12:01 PM (11 years, 4 months ago)

In my own cardboard experiments I am notising a deffinite difference in qualities of cardboards, There is the darker stuff which is good and the lighter very recycled crap which does not work well, unfotrtunately it seems like here we mostly only get the lighter recycled crap .

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Re: cardboard on agar? [Re: Lennybernadino]
    #17546758 - 01/14/13 12:03 PM (11 years, 4 months ago)

Quote:

Lennybernadino said:
In my own cardboard experiments I am notising a deffinite difference in qualities of cardboards, There is the darker stuff which is good and the lighter very recycled crap which does not work well, unfotrtunately it seems like here we mostly only get the lighter recycled crap .




This is an interesting observation, I had given up on cardboard and egg cartons because it always seemed to slow down and stall after a week, but it was always recycled materials.  Makes sense now that I think about it, I'm sure the recycling process takes a lot of the nutrition out.


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Re: cardboard on agar? [Re: RiparianZoneJunky]
    #17546803 - 01/14/13 12:10 PM (11 years, 4 months ago)

Maybe it would help to soak the cardboard in a more nutritious solution than plain water, like weak coffee or compost tea. The calorie content of recycled cardboard should be the same even if nitrogen and minerals are lost.

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Re: cardboard on agar? [Re: thiotimoline]
    #17546818 - 01/14/13 12:13 PM (11 years, 4 months ago)

over the years ive eaten up a huge 52" TV box that was left in my garage when i bought this house! :smile: i like thick currogated cardboard the best. egg cartons are hit and miss. the darker the better.  oysters really like the purple 24 packs. i started with currogated , then went to egg cartons but im back to currogated again.
Quote:

thiotimoline said:
Maybe it would help to soak the cardboard in a more nutritious solution than plain water, like weak coffee or compost tea. The calorie content of recycled cardboard should be the same even if nitrogen and minerals are lost.




that may invite contams since you only pasturize cardboard which its self is a pretty contam free material. especially if your working with slower species than oysters.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17546842 - 01/14/13 12:17 PM (11 years, 4 months ago)

Good point. You'd have to make it weak enough that you only get back to the nutrition level of normal cardboard, not any higher. Might not be worth the effort of calibrating this.

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Re: cardboard on agar? [Re: thiotimoline]
    #17546870 - 01/14/13 12:24 PM (11 years, 4 months ago)

ya like the serbs took a month to colonize a small piece of cardboard. it woulda been a trich farm by then.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17845380 - 02/22/13 12:54 AM (11 years, 2 months ago)

So to keep this going i just put some pasturized cardboard onto dishes of P subaeruginosa, P cyanfibrillosa, P quebecensis , P allenii and P caerulipes. :smile:

oh and to update those other bags, the one ovoid turned into an ovoid bonsai and although i only took pics of one of the bags, but i had 2 serbica bags .one of the serbicas was made into a bonsai and the other has been doubled up now and is a healthy fat bag of bohemica spawn ready for spring innoculations!! :rockon:

Edited by psylosymonreturns (02/22/13 12:57 AM)

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Re: cardboard on agar? [Re: psylosymonreturns]
    #17846368 - 02/22/13 09:34 AM (11 years, 2 months ago)

I look forward to the results!


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Re: cardboard on agar? [Re: Javadog]
    #17889077 - 03/01/13 09:25 PM (11 years, 2 months ago)

this is turning out to be an interesting experiment.

it turns out the hardest one to germinate, and the slowest and weakest of the latest 5 , is P quebecensis. But out of all 5 it has taken to the cardboard the fastest.
similiar to how the cardboard saved me with the other slow weak species P seribca. :thumbup: P caerulipes which is similar and seems to be taking as well. so far only P allenii and P cyanfibrillosa havent taken to the cardboard.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17889222 - 03/01/13 09:44 PM (11 years, 2 months ago)

I will keep this in mind bro.  I am restarting serbica.  ...though
I am about four months behind schedule on it.

Good luck,

JD


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Re: cardboard on agar? [Re: Javadog]
    #17889392 - 03/01/13 10:05 PM (11 years, 2 months ago)

my serbica is deadly now. once its a mass it really strenghtens up. i have started mixxing more sawdust now with my chips as it rips through it much faster with more smaller partilces.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17890388 - 03/02/13 01:33 AM (11 years, 2 months ago)

I find P. quebecensis is really weak on agar, i have yet to isolate a clean strong culture.
:nyan:


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Re: cardboard on agar? [Re: Joust]
    #17890448 - 03/02/13 02:02 AM (11 years, 2 months ago)

Quote:

Joust said:
I find P. quebecensis is really weak on agar, i have yet to isolate a clean strong culture.
:nyan:




i have a few decent isolates but i dont see this species ever getting really aggressive.
im making a quebec LC tomorrow.

hey where did you get yours? from nova scotia?


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17891412 - 03/02/13 10:14 AM (11 years, 2 months ago)

Quote:

psylosymonreturns said:
Quote:

Joust said:
I find P. quebecensis is really weak on agar, i have yet to isolate a clean strong culture.
:nyan:




i have a few decent isolates but i dont see this species ever getting really aggressive.
im making a quebec LC tomorrow.

hey where did you get yours? from nova scotia?



yeah from a microscopy sample.
:nyan:


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Re: cardboard on agar? [Re: Joust]
    #17891419 - 03/02/13 10:17 AM (11 years, 2 months ago)

cool we got the same ones. :smile: its a race now boooy!!


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17891424 - 03/02/13 10:18 AM (11 years, 2 months ago)

Quote:

psylosymonreturns said:
cool we got the same ones. :smile: its a race now boooy!!



i ied mine are from maine.. but i declare you the winner.
:nyan:


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Re: cardboard on agar? [Re: Joust]
    #17891447 - 03/02/13 10:24 AM (11 years, 2 months ago)

yours were from Maine? what observation?


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17891457 - 03/02/13 10:25 AM (11 years, 2 months ago)

ill see if i can find it, it was a while ago.
:nyan:


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Re: cardboard on agar? [Re: Joust]
    #17891470 - 03/02/13 10:29 AM (11 years, 2 months ago)

Ahh i cant find it...
:nyan:


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Re: cardboard on agar? [Re: Joust]
    #17891482 - 03/02/13 10:32 AM (11 years, 2 months ago)

hmmm wierd i dont remember that one from Maine.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17891526 - 03/02/13 10:48 AM (11 years, 2 months ago)

ill try to keep finding it. It was a shroomery observation, this last fall i believe.
:nyan:


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Re: cardboard on agar? [Re: Joust]
    #17891548 - 03/02/13 10:53 AM (11 years, 2 months ago)



--------------------
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"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       

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Re: cardboard on agar? [Re: Joust]
    #17891653 - 03/02/13 11:15 AM (11 years, 2 months ago)

blammo, there ya go. i have that print on paper in that pic! :lol: fuck i forgot how cute them little honeys are!! this reexcites me! :rockon:


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17913745 - 03/06/13 02:49 PM (11 years, 2 months ago)

Quote:

psylosymonreturns said:
blammo, there ya go. i have that print on paper in that pic! :lol: fuck i forgot how cute them little honeys are!! this reexcites me! :rockon:




Just a suggestion but since there are 3 of you working with psilocybe quebecensis maybe you guys can start a new thread as to combine your knowledge and resources.
I have to confess I am also interested and this way I can follow the progress without bugging you guys.


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Re: cardboard on agar? [Re: wolfedawwg]
    #17917959 - 03/07/13 10:02 AM (11 years, 2 months ago)

Oh there will be a Quebec thread soon enough. :wink:

This is more about the card board on agar in general. and the quebecs just happen to be the star of the show atm.!!

its amazing how this species wakes up when it comes in contact with cardboard!! its a very non exciting mycelium to grow, its slow, its not very thick and rhizomorphic. but that all changes!! its lightyears ahead of the 4 other species i am experimenting with. and subaeruginosa and my allen were ultra thick and vigorous and are waaaaay stronger species in general.

if i have time i'll get pics later.


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17965250 - 03/16/13 05:21 PM (11 years, 2 months ago)

So I noticed my cyanfibrillosa finally has taken to the cardboard too. ive had a hard time with this species so im hoping this will be my savior!

and i am planning on taking the quebecensis ,subaeruginosa and allenii cardboard off the plates and into some woodchip/dust mix. i will get pics when i do. :smile:


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Re: cardboard on agar? [Re: psylosymonreturns]
    #17966894 - 03/17/13 01:08 AM (11 years, 2 months ago)

Thanks for the update.

:thumbup:


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Re: cardboard on agar? [Re: Javadog]
    #18011316 - 03/25/13 09:41 PM (11 years, 1 month ago)

Here is P quebecensis. it really likes cardboard!!

and P allenii cardboard, off the plate and into some sterilized sawdust and woodchips.


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