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Javadog
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Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: cardboard on agar? [Re: inski]
#16930393 - 09/29/12 12:09 AM (11 years, 7 months ago) |
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I have found Serbica to be less than speedy/strong myself.
I will try this too.
I also found egg cartons to rock Mateo. I like to open the ziplock bags once in a while in front of a flowhood to freshen them up.
Take care,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: stonesun]
#16931297 - 09/29/12 07:27 AM (11 years, 7 months ago) |
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Quote:
stonesun said: Actually P. serbica var. bohemica is doing pretty well on standard MEA in my experience.
Well they are not that slow now that I've isolated. But compared to my other projects I find P bohemica to be a bit slow. Like compared to P ovoid or P azure they are very slow and not as thick and rhizomorphic.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Yes, I believe the sublignicolous or secondary decomposers of wood are just naturally slow growing compared with their lignicolous cousins like P. ovoideocystidiata or P. azurescens making them a bit more difficult to cultivate!
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: inski]
#16933997 - 09/29/12 06:02 PM (11 years, 7 months ago) |
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Hmm i didnt realize P bohemica was a secondary decomposer. it makes sense though now that ive seen the mycelium.
and you mention it is in the section Aztecorum? So like P baeocystis ? So are all the species in that section secondary decomposers? so what substrate would you recommend ? i was going to try just straight wood chips like all other woodlovers . but maybe i should be using a substrate more suited for P caerulescens or P baeocystis.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Quote:
psylosymonreturns said: Hmm i didnt realize P bohemica was a secondary decomposer. it makes sense though now that ive seen the mycelium.
and you mention it is in the section Aztecorum? So like P baeocystis ? So are all the species in that section secondary decomposers? so what substrate would you recommend ? i was going to try just straight wood chips like all other woodlovers . but maybe i should be using a substrate more suited for P caerulescens or P baeocystis.
Oh, sorry, that was a misunderstanding, P. serbica is in section Cyanescens stirps Serbica, species in that group are generally secondary decomposers of wood, you should try fairly old wood and sawdust from trees that are similar to what the species fruits from in nature, probably conifers, it may colonise fresh wood but I doubt it will fruit from that for a few years, the wood needs to be old enough so that you can't smell the terpines anymore.
The species I am working with belongs in section Aztecorum, most species there are sublignicolous.
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Javadog
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Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: cardboard on agar? [Re: inski]
#16934181 - 09/29/12 06:40 PM (11 years, 7 months ago) |
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I wonder if a spent shitake block might be useful for subs?
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: cardboard on agar? [Re: Javadog]
#16934334 - 09/29/12 07:07 PM (11 years, 7 months ago) |
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Quote:
Javadog said: I wonder if a spent shitake block might be useful for subs?
Hmm, not sure if it could colonise something that is already completely colonised by another fungus, maybe if you broke up the block, put it in jars and sterilised it again it could work, it's worth a try!
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Javadog
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Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: cardboard on agar? [Re: inski]
#16934545 - 09/29/12 07:49 PM (11 years, 7 months ago) |
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Quote:
inski said:
Quote:
Javadog said: I wonder if a spent shitake block might be useful for subs?
Hmm, not sure if it could colonise something that is already completely colonised by another fungus, maybe if you broke up the block, put it in jars and sterilised it again it could work, it's worth a try!
That is what I was thinking.
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: Javadog]
#16935054 - 09/29/12 09:47 PM (11 years, 7 months ago) |
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i just collected some 2 -3 year old conifer chips that have had C lagopus, A praeocox and G luteofolius already rip through it. it looks to be free of any mycelium now as it dies back over the dry summer and i only collected from the top few inches. and once i sterilize i hope it will be a good substrate.
ive done unsterile work with other wood lovers, like P cyanescen and P azurescens and even SRA and i could cut a hazel nut or alder down and have it colonized in a few weeks. in my experience fresher the better. but honestly P bohemica is not as vigorous as even P cyanescen.P azurescen and SRA on the other hand are hands down the fastest most intense colonizers. P ovoids seems to be on the same level.
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lipa

Registered: 07/24/07
Posts: 2,684
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Re: cardboard on agar? [Re: inski]
#16935782 - 09/30/12 01:13 AM (11 years, 7 months ago) |
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Quote:
inski said:
Quote:
Javadog said: I wonder if a spent shitake block might be useful for subs?
Hmm, not sure if it could colonise something that is already completely colonised by another fungus, maybe if you broke up the block, put it in jars and sterilised it again it could work, it's worth a try!
I think what Javadog is implying is that the mushroom species does not like substrates with the lignin still intact. Maybe it relies on other fungi to "open it up" like Vollvariiella bombycina for example.
Lipa
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: cardboard on agar? [Re: lipa]
#16936033 - 09/30/12 02:39 AM (11 years, 7 months ago) |
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Yes, I knew that was what he was implying but I thought he wanted to use the spent substrate without treating it, I believe it would need to be sterilised then inoculated with the sublignicolous species, it is a good idea and I hope he experiments and documents his findings.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: inski]
#16937382 - 09/30/12 11:12 AM (11 years, 7 months ago) |
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Oh I would give ole JD the benefit of the doubt. I know he knows he has to resterilize.
Ive read Stamets talking about using spent shiitake blocks resterilized for oyster growing. So its something thats done, not sure with these species were talking about though.
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Javadog
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Registered: 05/03/10
Posts: 7,385
Loc: USA
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Yup. Thank you both for the good word.
Indeed, given the learning I need to do, I will vary the approach, by supplementing some of the blocks of semi-decomposed sawdust with chopped straw and separately rice hull, and then a variety of other standard supplements. (bran, coffee, gypsum, etc)
The other thing that I need to do is to setup the mini fridge that I have mentioned elsewhere. I have been percolating on the idea of doing this since I wrecklessly purchased Azures (Hammond Flying Saucers from a sponsor) as part of my very first purchases. Boy, I was ahead of myself that time, but I will need to push this to the next level.
This is an inspiring thread.
Thanks for sharing!
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: Javadog]
#16943404 - 10/01/12 08:54 AM (11 years, 7 months ago) |
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Quote:
Javadog said: Yup. Thank you both for the good word.
Indeed, given the learning I need to do, I will vary the approach, by supplementing some of the blocks of semi-decomposed sawdust with chopped straw and separately rice hull, and then a variety of other standard supplements. (bran, coffee, gypsum, etc)
The other thing that I need to do is to setup the mini fridge that I have mentioned elsewhere. I have been percolating on the idea of doing this since I wrecklessly purchased Azures (Hammond Flying Saucers from a sponsor) as part of my very first purchases. Boy, I was ahead of myself that time, but I will need to push this to the next level.
This is an inspiring thread.
Thanks for sharing!
JD
ive been contemplating putting one of my azure bonsais in the garage fridge to see if a good cold shock can start an early flush. i dont expect them to flush naturally until like november. last year they fruited outside until new years!
but an actual grow in the fridge would be really cool. i keep my eyes on craigslist to see if theres ever one of those glass door coolers you see in stores. growing azures inside could be a challenge but just growing the myc is VERY easy. it is ultra vigorous!!
not sure if you noticed mdma's indoor P cyanescen flush in the hunting forum! he had a box of colonized chips sitting above a cupboard in the kitchen and the fucking things started to fruit!!
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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I did see that...amazing.
Never say impossible when it comes to fungi. (remember the pumpkin guts grow ;0)
Take care,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: Javadog]
#16944260 - 10/01/12 11:26 AM (11 years, 7 months ago) |
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yup hard to predict sometimes. 
so back to the original topic i present some cardboard on agar.
Blue oyster . As you can see from the bottom shot i took sections out of the middle of the plate. leaving some thick edges. Ive read to be safe dont inoculate with the myc along the edge as its most likely to be closer to contams. but throw a picece of cardboard in there and it goes crazy since it is the most vigorous leading edge anyways and its a shame to waste it! 
 
and i guess were calling this P serbica now.From Greece.

and heres a comparison of P serbica myc and P ovoid myc. although the ovoid is on its 3rd and serb is on its 2nd transfer. but there is clearly a difference in vigor and apearance. and just to be clear this is my very first time doing this stuff and my very first pours . my plates were on an angle and colected on the one side hence the side inoculations. i'll do it better next time. 


i keep my plates in ziplocs? does everyone do that?
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Edited by psylosymonreturns (10/01/12 07:02 PM)
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Javadog
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Registered: 05/03/10
Posts: 7,385
Loc: USA
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Lord, that ovoid mycelium is beautiful. :0)
I do not bag my dishes....probably should, but I have soooo many going at one time. (and they are impossible to stack)
Good stuff. Thanks for sharing.
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: Javadog]
#16949339 - 10/01/12 11:16 PM (11 years, 7 months ago) |
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Thanks man I need to Noc something up with that beauty soon!!
Today I used that oyster cardboard to Noc up some pasteurized cardboard and coffee grounds.So that will be the first grain skipper project. The myc was super thick on that cardboard so I'll give a update in a week.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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so just to update this thread and not leave yall hanging, here is the work in progress.
this is the carbaord i had put on the used P ovoid and P serbica agar plates. once the cardbaord was colonized i transfered pieces to boiled hardwood chips.
here it is. yes it works.  P ovoid
 P serbica

to be honest my serbica project would have been screwed if i didnt do this as everything else fizzled out for me.
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Lennybernadino
Amazon grower


Registered: 09/16/09
Posts: 772
Loc: Iquitos, Peru
Last seen: 27 days, 4 hours
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Nice! Its really cool to see the simple natural tenchnique succede where the more advanced ones failed!
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