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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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cardboard on agar? 1
#16855401 - 09/16/12 10:07 AM (11 years, 7 months ago) |
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So I tried searching about this idea but I couldnt really find what I was looking for . And I dont consider myself advanced in the slightest, but I cant ask questions in the cult forum because they are cube nazis.
Soooooooo,
I ve been dabbling in the arts of spore germination on agar finally . And have been having good success for a first timer. So I streaked the plate with a wild Oregonian P ovoid. I followed what i read and transfered a thick leading edge of growth that looked the strongest and went on with my business.
So I didnt throw out that dish . And it never got any contams and had tons of thick growth so I didnt want to waste it. So i boiled some cardboard and in my glove box put a piece of soaked carboard rigt inside the dish with all those different strains fighting for dominance .
So its been a while and there is thick rhizomorphic strands of ovoid myc eating that cardboard from all sides. I tried it with the P bohemica plate i transfered off of and its looking good too . 
Obviously others have done this I imagine . Is it stupid to do shit like this ? Should I be chucking those first plates all the time ? And would these strands of myc attacking the cardboard be considered worthy of growing out ? I am planning on just adding that myc to egg cartons and boiled hardwood chips and making some ovoid bonsai pots and see what happens in the spring.
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blackdogs
fart in the wind
Registered: 02/12/12
Posts: 241
Last seen: 11 years, 7 months
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that's great. i'm glad to hear the cardboard thing really works.
"Obviously others have done this I imagine . Is it stupid to do shit like this ? Should I be chucking those first plates all the time ? And would these strands of myc attacking the cardboard be considered worthy of growing out ? I am planning on just adding that myc to egg cartons and boiled hardwood chips and making some ovoid bonsai pots and see what happens in the spring. "
no way. i think it's a smart thing to do. you'll be pioneering these techniques. : )
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: blackdogs]
#16861509 - 09/17/12 10:01 AM (11 years, 7 months ago) |
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I just know, well from what ive read and ive read for many years before taking this stuff up. is that you dont want any excuse to invite contams into your scenario . but ive been diligent and also a bit lucky for a newb i think.
but the one benefit i can see from this is skipping the expensive grain . cardboard is free and wood chips are free so for the poor grower you can cut the cost of grain.
although grain gets colonized so damb fast its still worth it if you can afford it.
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,037
Loc: The Island of Misfit Toys
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I don't see anything wrong with your idea. In fact it inspired me to place a piece of cardboard (soaked under the faucet for a minute and then a quick dowsing of peroxide) onto a petri that I just spent taking isolates from. I like experimenting. 
Keep us updated on your progress.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: MacMerdin]
#16870260 - 09/18/12 05:48 PM (11 years, 7 months ago) |
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cool man!! what species are you trying this with ?
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,037
Loc: The Island of Misfit Toys
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For the experiment: ovoids.
This petri had three sectors on it so I'd imagine that I can get to a near isolate if it works?
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: MacMerdin]
#16870982 - 09/18/12 07:55 PM (11 years, 7 months ago) |
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Nothing wrong with working with woodlovers! Are you going to spawn from the cardboard to wood and then make an outdoor patch?
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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ya thats the plan. i also like growing out woodlovers in bonsai pots as they are easy to keep watered with your other plants .
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 9 months
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Great idea, I'll have to try this. I've got a patch of ovoids going that I transferred from stembutts. I'm hoping it will fruit this spring, but I've heard they can take a year or two. Good luck!
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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it really all depends how vigourous your myc is. a patch should fruit after a year if it didnt die and ive made bonsais in the spring that fruited by the fall. its a smaller space to colonize if you want fruits right away . its different when you put it in the ground though. ive lost more myc than ive had fruit in the ground.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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so i took the piece of cardboard off the ovoid dish the other day since it was almost fully covered. i mixxed it with more boiled cardboard. once those pieces are colonzed i will add wood chips.
so i nocced up some grain the other day with some blue oyster myc . there was some remaining myc along the edges so i put some boiled cardboard in that dish. well its already spreading across the cardboard. obviously oysters wouid work but hey gotta try.
Mods feel free to move this somewhere more apropriate since this is not really advanced stuff !
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Mateo
High on LIFE!



Registered: 06/24/11
Posts: 2,695
Loc: North Europe
Last seen: 10 days, 5 hours
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I think there is much more benefit to grow woodlover mycelium on cardboard/eggkartons then the cost of grains.
When you want to increase mycelium mass of woodlovers for a grow you will want to colonize woodchips. Now, the woodlover mycelium on the agarplate or grains won't colonize the woodchips as fast as mycelium grown on cardboard/eggkartons. This is because the mycelium needs to "get used to" the new substrate wood. This halt in growth happens all the time when you change to an unknown substrate for the mycelium. The cardboard/eggkartons is made of woodmaterial and the mycelium can go right into colonization mode without halting when you mix with the woodchips later.
I have a couple of eggkartons that i pasturized, and in each "pit" i put a couple of colonized ryegrains (P.Azurescens). The mycelium has now spread and have colonized about 50% of the eggkartons. I keep the eggkartons in plastic bags to keep the moisture and have only opened the bags once for airexchange and a little misting.
So far i havent thought about what to do with the mycelium but i think i keep expanding the mycelium mass until spring and make a nice outdoor patch and maybee a small indoor experiment.
Woodlovers is so nice, i might try some others besides Azures also.
-------------------- A wise rat has many holes
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: Mateo]
#16924051 - 09/27/12 11:01 PM (11 years, 7 months ago) |
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ya its going good, cardboard is very resistant to contams and to me using the plate after youve already transfered sectors to grain , is well worth it. the oyster dish is thick just from the edges of the plate eating up the cardboard. i think it may be spreading better on the cardboard then rye grain. the ovoids worked good and the P bohmeica is slow as always. but still no contams in any of my experimental dishes that ive transfered sections from.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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What type of agar recipe did you initially use for your Psilocybe bohemica experiments?
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Re: cardboard on agar? [Re: inski]
#16924360 - 09/28/12 12:01 AM (11 years, 7 months ago) |
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MEA.
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Lennybernadino
Amazon grower


Registered: 09/16/09
Posts: 772
Loc: Iquitos, Peru
Last seen: 27 days, 4 hours
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Why do you assume that others have obviously done this? It is amazingly easy to do something no one else has ever done , we may be billions over time but what we have discovered and done is finite and the universe and posssabilities are infinite . It is a common myth that we believe that it is difficult to come up with a new ide or invention and if you believe that myth you will not try, if you do not it is easy . It seems like you came up with a good shortcut for isolating the fruiting aggressive strains from the fruitless time wsting strains. Crdboard would work with many diffirent species, I wonder wich species would not be cardboard compatible, and what could be used with them. jEr
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 9 months
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Well thanks mate, maybe it is a new idea. so far its working great and I will try it with every plate that i transfer off of.it will be interesting to see which species it will work for.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Quote:
psylosymonreturns said: MEA.
Was the mycelium extremely slow on this medium?, if yes then you could try making a solution by boiling some aged sawdust from a similar type of tree to the ones that this species likes to fruit from in the wild, boil the sawdust for 20 minutes or so then strain the tea and use the water for your agar, I used this water with my usual MEA recipe but with about half the amount of malt extract on a species that was painfully slow on typical agar recipes and the mycelium almost doubled it's speed of growth!!
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stonesun
Sclerotia Aficionado




Registered: 05/22/09
Posts: 5,444
Loc: 64º08'00"N 21º56'00"W
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Re: cardboard on agar? [Re: inski]
#16929591 - 09/28/12 09:21 PM (11 years, 7 months ago) |
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Actually P. serbica var. bohemica is doing pretty well on standard MEA in my experience.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: cardboard on agar? [Re: stonesun]
#16929642 - 09/28/12 09:32 PM (11 years, 7 months ago) |
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Ok, nice, I was conversing with a friend who is having problems with it being very slow, I have also been working with a species from Section Aztecorum which is very slow on MEA and PDA but when transfered to an agar medium as described above the mycelium almost doubled in speed of growth, I also suspect that the growth is slowed if a large amount of spores are germinated in close vicinity to one another, they all compete for nutrients, as you do transfers and get close to a pure culture the growth speeds up, good luck with your wood lover experiments
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