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Offlineexploratory
Stranger
Registered: 09/12/12
Posts: 2
Last seen: 12 years, 1 month
Questions about first grow attempt
    #16849554 - 09/15/12 01:31 AM (12 years, 4 months ago)

So, I've decided to get into this hobby and got hold of some GT spores.

I didn't do quite enough reading before my first attempt, and made up 3 BRF cakes of approximately 1 quart volume each (which I now realise is too big). I also think my dry verm layer was too thick compared to the length of the needle I used at that time, so the spores never reached the substrate. No growth occurred in any of them.

I also made a couple of LCs at this point however, which definitely grew something. I'm now doubting that they actually grew mycelium though.

I then made a second attempt, involving 4 half-pint glasses using rye flour cakes. I inoculated 2 of them with spores, and the other two with my LC. I also decided I had nothing to lose and re-inoculated the 3 original containers with LC as well.

Fast forward about 10 days to the present time, and the 2 half-pint rye flour cakes with spores are doing well, both about 60% colonized with healthy white mycelium.

Still no growth whatsoever (and no apparent contaminants) in any of the other containers that I inoculated using LC though.

I'm thinking my LCs might have somehow both grown a yeast in them instead of mycelium, as they have no obvious smell and have definite growth of something, but the growth seems denser, clumpier, and more tan coloured than pictures I've seen of healthy mycelium LCs. Whatever it is hasn't shown any sign of growing on any of the cakes inoculated with it either, which would be consistent with a yeast.

Here's a picture of what one of the LCs looks like when allowed to settle:
http://imgur.com/fIzIH

and when swirled gently:
http://imgur.com/fAR6q

What are your comments on the appearance of this LC?

Also, my plan at this point is to grow the 2 successful cakes like normal, then attempt to make new LCs using a sterile water syringe washout of the jars just prior to birthing the cakes. I'll then fruit the cakes and could re-attempt cloned tissue LCs or tissue grown out on peroxide agar as a basis for starting LCs. Basically I live in a country with strict customs laws where spores are very hard to get, and want to establish viable LCs as a means of starting new growth.

I have also toyed with the idea of smashing up the spent cakes after fruiting and spawning them as cleanly as possible into a tub together with all of the apparently un-colonised cakes as a bulk substrate. I know this will risk contamination but I figure I have nothing to lose by giving it a go and it could produce fruits if it works out ok.

Thoughts?

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Invisiblejenkinz
Parliament of Trees Ambassador
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Registered: 04/02/12
Posts: 523
Re: Questions about first grow attempt [Re: exploratory]
    #16849569 - 09/15/12 01:38 AM (12 years, 4 months ago)

That LC doesn't look too good IMO.

As for toying with your 'uncolonized' cakes, consider that if they are contaminated you could very well be introducing that to your home and grow area and ruin any future attempts. Also potential health risks given that you have no idea what's growing in there.

Find a tek and follow it exactly. When things go wrong you can look back and see why, learn from it and start over. Toss those old jars


--------------------

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OfflineCosmic Fool
quantum menace
Registered: 09/07/12
Posts: 92
Last seen: 12 years, 3 months
Re: Questions about first grow attempt [Re: jenkinz]
    #16849582 - 09/15/12 01:45 AM (12 years, 4 months ago)

I'd echo that sentiment of the LC being off.  Doesn't look right.  I also wouldn't mess around mixing spent cakes and contaminated ones, as it'll most likely only prove a waste of time, as well as leading to other issues already above mentioned.

Cloning a pin from a cluster to agar then transfering clean growth to liquid would be an easy way to get an lc from this point.

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Offlineexploratory
Stranger
Registered: 09/12/12
Posts: 2
Last seen: 12 years, 1 month
Re: Questions about first grow attempt [Re: Cosmic Fool]
    #16849608 - 09/15/12 01:55 AM (12 years, 4 months ago)

Thanks for the input, yeah, I'm thinking that transferring some pin tissue to peroxide agar will be a good way to get a clean sample of mycelium to start future LCs from.

Point taken about the old cakes.

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