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InvisibleGuy1980

Registered: 09/11/12
Posts: 723
LC jar construction
    #16845006 - 09/14/12 06:14 AM (12 years, 4 months ago)

If I've researched right, my LC jar requires an injection port and a filter to allow the contents to breathe.  I plan to use a RTV silicone for the injector port - this much I'm sure about.

My question is, can I just drill holes and use micropore tape for filters?  A quick search shows people use various methods, but no-one seems to think one layer of micropore is sufficient.  Isn't two layers of micropore like putting two johnnies on your schlong, i.e. once you've got one on, you're as protected as can be?

Thank you in advance :smile:

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OfflinePussyFart
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Re: LC jar construction [Re: Guy1980]
    #16845030 - 09/14/12 06:29 AM (12 years, 4 months ago)

first of all...if u put 2 "johnnies" of the friction will break them both....

Micropore tape sucks because its flat(contams walk right in) and because it restricts GE(Gas Exchange) in some cases. Same with Tyvek.

The best method is a synthetic filter disc siliconed over a hole in the lid.

Polyfill"(Synthetic Pillow Stuffing) is also great, just stuff some thru the hole...u can even inoculate thru it.

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InvisibleGuy1980

Registered: 09/11/12
Posts: 723
Re: LC jar construction [Re: PussyFart]
    #16845046 - 09/14/12 06:39 AM (12 years, 4 months ago)

Quote:

Notahacker420 said:
Micropore tape sucks because its flat(contams walk right in) and because it restricts GE(Gas Exchange) in some cases. Same with Tyvek.

The best method is a synthetic filter disc siliconed over a hole in the lid.

Polyfill"(Synthetic Pillow Stuffing) is also great, just stuff some thru the hole...u can even inoculate thru it.




I'm glad you put that last sentence in - I thought people were referring to this stuff :laugh:



I'm just desperately trying to get the right stuff together to make a LC from my 2cc's of spores.  But further reading has shown it's difficult to succeed trying to innoc. LC with spores.

Ho-hum...

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InvisibleIveBeenRecycled
I like pie.
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Registered: 12/04/11
Posts: 653
Loc: Under the mango tree.
Re: LC jar construction [Re: Guy1980]
    #16845116 - 09/14/12 07:22 AM (12 years, 4 months ago)

As far as my LCs have gone, I prefer to make an injection port and then use a synthetic filter disc to provide the extremely minimal GE that is arguabl required. That being said, I've had many dozens of successful LCs without utilizing any kind of GE whatsoever. In our experience, you can't stop the fungus from growing....it's very hardy.


--------------------
I feel good.

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OfflinePussyFart
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Re: LC jar construction [Re: IveBeenRecycled]
    #16845254 - 09/14/12 08:24 AM (12 years, 4 months ago)

There is no argument...all mushroom mycellium needs GE, the amount can be argued, but the fact that mycellium needs it cannot.

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InvisibleGuy1980

Registered: 09/11/12
Posts: 723
Re: LC jar construction [Re: IveBeenRecycled]
    #16845343 - 09/14/12 08:59 AM (12 years, 4 months ago)

Quote:

Notahacker420 said:
Micropore tape sucks because its flat(contams walk right in) and because it restricts GE(Gas Exchange) in some cases. Same with Tyvek.

The best method is a synthetic filter disc siliconed over a hole in the lid.




Isn't a synthetic filter disc flat?  I'm thinking I can cut a filter out of a filter bag and silicone that down as my filter, but I don't want to waste a filter bag on something which offers no better protection than micropore.

Is cutting a filter from a filter bag and silicone-ing it down a suitable filter for an LC jar?


Quote:

IveBeenRecycled said:
As far as my LCs have gone, I prefer to make an injection port and then use a synthetic filter disc to provide the extremely minimal GE that is arguabl required. That being said, I've had many dozens of successful LCs without utilizing any kind of GE whatsoever. In our experience, you can't stop the fungus from growing....it's very hardy.




It's good to know you can get away without GE, and it raises the question is it better to have no GE than a poor filter (like my micropore over a hole).?

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OfflineSoreSpore
Registered: 03/06/12
Posts: 7,481
Loc: Halfway there...
Last seen: 10 years, 10 months
Re: LC jar construction [Re: Guy1980]
    #16845352 - 09/14/12 09:02 AM (12 years, 4 months ago)

Personally, I like just one hole in the center of the lid filled with RTV. This creates a SHIP (self healing injection port) and you can shake the jar as much as you need. Also, you will need to release the vacuum created by the PC. You can just use an empty syringe in a glovebox or in front of a flowhood. For open air inoculations, I use a syringe stuffed with polyfill, release the vacuum and inoculate with clone or ms syringe.

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OfflinePussyFart
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Re: LC jar construction [Re: PussyFart]
    #16845354 - 09/14/12 09:03 AM (12 years, 4 months ago)

Yes, filter discs are flat, but they are filters, made specifically for filtering, and they are thicker than tape or tyvek......oh yea and they are proven to work. :thumbup:

and



Quote:

Notahacker420 said:
There is no argument...all mushroom mycellium needs GE, the amount can be argued, but the fact that mycellium needs it cannot.



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OfflineSoreSpore
Registered: 03/06/12
Posts: 7,481
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Re: LC jar construction [Re: PussyFart]
    #16845359 - 09/14/12 09:05 AM (12 years, 4 months ago)

Quote:

Notahacker420 said:
There is no argument...all mushroom mycellium needs GE, the amount can be argued, but the fact that mycellium needs it cannot.



I use a lid, rubber seal down with no gas exchange port, just an RTV SHIP and the LC always colonizes fine.

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OfflinePussyFart
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Re: LC jar construction [Re: SoreSpore]
    #16845364 - 09/14/12 09:07 AM (12 years, 4 months ago)

Weird...maybe LC doesnt require that much GE....How much free space do u have in the top of the LC? (above the water under the lid)

I beleive it was RR that said that.

Edited by PussyFart (09/14/12 09:07 AM)

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InvisibleGuy1980

Registered: 09/11/12
Posts: 723
Re: LC jar construction [Re: PussyFart]
    #16845370 - 09/14/12 09:10 AM (12 years, 4 months ago)

Quote:

SoreSpore said:
Personally, I like just one hole in the center of the lid filled with RTV. This creates a SHIP (self healing injection port) and you can shake the jar as much as you need. Also, you will need to release the vacuum created by the PC. You can just use an empty syringe in a glovebox or in front of a flowhood. For open air inoculations, I use a syringe stuffed with polyfill, release the vacuum and inoculate with clone or ms syringe.




No glovebox or polyfill :frown:  I could sterilize with the lid loose and let it cool before tightening to erradicate the vacuum issue.  RTV based SHIP is what I plan to do otherwise though:thumbup:

Quote:

Notahacker420 said:
Yes, filter discs are flat, but they are filters, made specifically for filtering, and they are thicker than tape or tyvek......oh yea and they are proven to work. :thumbup:





So a filter stolen from a filter bag should be as good as a filter disc (I don't have any filter discs)?

Thanks all, any knowledge contributed is appreciated :smile:

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OfflinePussyFart
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Re: LC jar construction [Re: PussyFart]
    #16845377 - 09/14/12 09:12 AM (12 years, 4 months ago)

should work, should be the same thing

Edited by PussyFart (09/14/12 09:13 AM)

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InvisibleDynGBreeD
┌∩┐(◣_◢)┌∩┐

Registered: 01/15/11
Posts: 3,639
Re: LC jar construction [Re: SoreSpore]
    #16845379 - 09/14/12 09:12 AM (12 years, 4 months ago)

Quote:

SoreSpore said:
Quote:

Notahacker420 said:
There is no argument...all mushroom mycellium needs GE, the amount can be argued, but the fact that mycellium needs it cannot.



I use a lid, rubber seal down with no gas exchange port, just an RTV SHIP and the LC always colonizes fine.




I use the same lids (RTV), a few of them with no other holes.
And A few with Tyvek 1/4 inch holes with the SHIP. And I've never
seen a real difference :shrug: I know I don't care for Tyvek though.


--------------------

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