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OfflineMycoFathom
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Registered: 06/26/12
Posts: 71
Loc: United
Last seen: 11 years, 4 months
Need Guidance. please help
    #16831626 - 09/11/12 08:52 PM (12 years, 4 months ago)

I am researching ways for liquid culture techniques. I am thinking of using the honey LC Tek. If there is a better one out there please let me know. I found a 4 inch luer lok needle but it is a blunt tip. Where would I find one with a pointed tip? If not then how would I go about sharpening it so that I can puncture the injector port? Planning on using syringe filter and heavy duty injector port on Kerr pint jars.

Any thoughts, comments, better methods, ideas would be greatly appreciated. Thanks fellas.

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Offlinezepski
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Registered: 10/01/11
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Re: Need Guidance. please help [Re: MycoFathom]
    #16831648 - 09/11/12 08:55 PM (12 years, 4 months ago)

You will get mixed replies on the best LC.  Some like honey, some like, karo, some like Light malt extract.  Its all in what you like.  I dont know how to advice sharping the 4" needle otther then a grinder or something similar.  But since you are using a syringe filter for GE you shouldnt need the 4" needle just tilt the jar and use a normal needle.  Also check out: https://www.shroomery.org/forums/showflat.php/Number/14186384#14186384

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OfflineCosmic Fool
quantum menace
Registered: 09/07/12
Posts: 92
Last seen: 12 years, 3 months
Re: Need Guidance. please help [Re: MycoFathom]
    #16831675 - 09/11/12 08:59 PM (12 years, 4 months ago)

You'd find one at a pharmacy.  The blunt tip is an issue indeed.  I'd go with a 16 gauge probably for LC.. 2 inch usually is long enough for most purposes.

Honey is good. Light malt/dextrose is good too, as are other recipes.  Just keep the sugar content below 4% no matter what you choose (2% is more than enough ime).

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Invisiblewildernessjunkie
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I'm a teapot


Registered: 06/13/10
Posts: 8,118
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Re: Need Guidance. please help [Re: Cosmic Fool] * 1
    #16831745 - 09/11/12 09:13 PM (12 years, 4 months ago)

MS inoculated LC is an excellent way to ruin a grow.

Try Grain LC instead. - http://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=6817701&fpart=1&PHPSESSID=

Or better yet, just use the spores as is.

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OfflineCosmic Fool
quantum menace
Registered: 09/07/12
Posts: 92
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Re: Need Guidance. please help [Re: wildernessjunkie] * 1
    #16831758 - 09/11/12 09:15 PM (12 years, 4 months ago)

Yea, ms lc is pretty dicey.  Grain's a better bet for sure, or agar. With agar, you can inoculate a dish with syringe or print and make sure of what you are growing before transferring to lc. Grain's probably the easier thing for a beginner, but neither are really very hard.

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OfflineTheDeathryder
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Re: Need Guidance. please help [Re: Cosmic Fool]
    #16831792 - 09/11/12 09:22 PM (12 years, 4 months ago)

I'd make an agar jar with one of the no pour teks on the site, start my spores on the agar, then when the culture is verified clean, add water to the jar and let the agar dissolve to form my LC :smile:

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OfflineHellyen
Just Strange.
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Registered: 07/26/12
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Re: Need Guidance. please help [Re: Cosmic Fool]
    #16831797 - 09/11/12 09:23 PM (12 years, 4 months ago)

I use Honey in my LC's. Consistent success.


--------------------
I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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Invisiblewildernessjunkie
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Re: Need Guidance. please help [Re: Hellyen]
    #16831903 - 09/11/12 09:42 PM (12 years, 4 months ago)

Quote:

Hellyen said:
I use Honey in my LC's. Consistent success.



Honey works fine. Karo works better. Better than that is SITR. ELME/Dextrose works best. As cited in this experiment.

http://www.shroomery.org/forums/showflat.php?Cat=&Board=255&Number=9591904&fpart=1&PHPSESSID=

The gamble here is when you inoculate via MS. Other than that LC works great.

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OfflineHellyen
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Re: Need Guidance. please help [Re: wildernessjunkie]
    #16831919 - 09/11/12 09:46 PM (12 years, 4 months ago)

Quote:

wildernessjunkie said:
Quote:

Hellyen said:
I use Honey in my LC's. Consistent success.



Honey works fine. Karo works better. Better than that is SITR. ELME/Dextrose works best. As cited in this experiment.

http://www.shroomery.org/forums/showflat.php?Cat=&Board=255&Number=9591904&fpart=1&PHPSESSID=

The gamble here is when you inoculate via MS. Other than that LC works great.




If you wanted me to read that... No Thanks. Just sayin Honey works I know other stuff does too.


--------------------
I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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Offlinezepski
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Re: Need Guidance. please help [Re: Hellyen]
    #16831938 - 09/11/12 09:49 PM (12 years, 4 months ago)

Maybe he was just adding to your point.  little touchy arent we? lol

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Invisiblewildernessjunkie
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Re: Need Guidance. please help [Re: Hellyen]
    #16831978 - 09/11/12 09:53 PM (12 years, 4 months ago)

Quote:

Hellyen said:
Quote:

wildernessjunkie said:
Quote:

Hellyen said:
I use Honey in my LC's. Consistent success.



Honey works fine. Karo works better. Better than that is SITR. ELME/Dextrose works best. As cited in this experiment.

http://www.shroomery.org/forums/showflat.php?Cat=&Board=255&Number=9591904&fpart=1&PHPSESSID=

The gamble here is when you inoculate via MS. Other than that LC works great.




If you wanted me to read that... No Thanks. Just sayin Honey works I know other stuff does too.




That wasn't for you. It was for the OP. Im hoping that he/she would like to learn something new. Also you should check your attitude.

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OfflineHellyen
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Re: Need Guidance. please help [Re: zepski]
    #16832000 - 09/11/12 09:56 PM (12 years, 4 months ago)

Not at all Zepski. Just thinking most households prolly have honey as opposed to karo. Sometimes it's about what works and is more accessable. No ill will wildjunkie :wink:


--------------------
I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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Offlinezepski
Ya Momma
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Re: Need Guidance. please help [Re: Hellyen]
    #16832043 - 09/11/12 10:02 PM (12 years, 4 months ago)

True i can say i didnt have anything needed for doing LC till i started doing LC, now i have karo, honey, LME, and turbinado sugar (sugar in the raw).  Out of all them i personally like LME showed the best quickest growth but will say i had more failures with it then my 2nd favorite which is sugar in the raw.  3rd would be karo.  only reason i dislike honey and rank it last is because I never had success with it and the sediment that comes with it.

But to each their own.  Like i said to OP before best to try several and see what you like the most.

But on the side note I have to agree with wildernessjunkie  on the GLC.  Its best way to be sure to have a contam free LC.

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Invisiblewildernessjunkie
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Re: Need Guidance. please help [Re: zepski]
    #16832094 - 09/11/12 10:10 PM (12 years, 4 months ago)

If you can do agar>grain>glc then you will be pretty much guaranteed clean. From the GLC you could also inoculate GLC > LC , and still be clean. But if you can do G2G then you could see spawn multiply like nothing you have ever seen before. And all it requires is a still air box.

There are only two, things that are required to make that happen too. A PC and a still air box.

Side note: Karo on pancakes is delicious. So is honey.

I think I'll make pancakes for breakfast tomorrow.

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OfflineMycoFathom
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Registered: 06/26/12
Posts: 71
Loc: United
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Re: Need Guidance. please help [Re: wildernessjunkie]
    #16836400 - 09/12/12 05:19 PM (12 years, 4 months ago)

alright fellas, thanks for the info. Another question, would I have the same results if I start a culture on agar using 1/2 pint jar and then move to liquid culture? rather than using grain culture. Also has anyone used the blue silicone injection port, and how many uses could I expect from one?

Thanks.

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OfflineCosmic Fool
quantum menace
Registered: 09/07/12
Posts: 92
Last seen: 12 years, 3 months
Re: Need Guidance. please help [Re: MycoFathom]
    #16838636 - 09/12/12 11:21 PM (12 years, 4 months ago)

The agar culture can be started directly from spore.. print or syringe is fine. Once you have a clean culture, a wedge from that can be transferred to liquid.

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Invisiblewildernessjunkie
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I'm a teapot


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Need Guidance. please help [Re: Cosmic Fool]
    #16838937 - 09/13/12 12:17 AM (12 years, 4 months ago)

Quote:

Cosmic Fool said:
The agar culture can be started directly from spore.. print or syringe is fine. Once you have a clean culture, a wedge from that can be transferred to liquid.




Agreed.

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OfflineMycoFathom
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Registered: 06/26/12
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Loc: United
Last seen: 11 years, 4 months
Re: Need Guidance. please help [Re: wildernessjunkie]
    #16839500 - 09/13/12 06:13 AM (12 years, 4 months ago)

What if I started the agar culture in a half pint jar and then add water to pull it into a syringe. Then expelling it into a liquid culture. Would this ruin the sugar content percentage in my Honey LC or would it be fine. Say around 2 cc for a pint of honey LC? I am in an area were contams pose a greater threat. The less I have to open things the better. Thanks for the input fellas.

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OfflineCosmic Fool
quantum menace
Registered: 09/07/12
Posts: 92
Last seen: 12 years, 3 months
Re: Need Guidance. please help [Re: MycoFathom]
    #16840484 - 09/13/12 10:48 AM (12 years, 4 months ago)

Yeah, that would work, but it's a bit overcomplicated. It wouldn't wreck the sugar content anything, but it'll add an extra step for things to go wrong at.  As long as you use a glovebox, you should be fine going straight from agar to liquid.  It's a pretty standard technique Besides, either way you'll end up having to open a jar lid.

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InvisibleRahz
Alive Again
Male


Registered: 11/10/05
Posts: 10,315
Re: Need Guidance. please help [Re: MycoFathom]
    #16840546 - 09/13/12 10:58 AM (12 years, 4 months ago)

I've used honey, karo and light malt/dextrose. They all work fine. I use honey now because I always have some at the house.

You can find needle tips at any tack supply (horse supply) for a few bucks.

These are the lids I use: EZ-LC lids. They can be built cheap and work the same as what you plan except you don't have a syringe flopping around on the top of the jar.


--------------------
rahz

comfort pleasure power love     truth awareness peace I am      I feel      I do     I love  I speak    I see    I know

"If you knew how quickly people forget the dead... you would stop living to impress people." — Christopher Walken

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OfflineHybridprX
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Re: Need Guidance. please help [Re: Rahz]
    #16840584 - 09/13/12 11:04 AM (12 years, 4 months ago)

Thats awefully strange that honey works for you guys since it contains a natural fungicide....I never had success with a LC using honey.

Karo works, Malt/dextrose is for the win.


--------------------

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InvisibleRahz
Alive Again
Male


Registered: 11/10/05
Posts: 10,315
Re: Need Guidance. please help [Re: HybridprX]
    #16841100 - 09/13/12 01:26 PM (12 years, 4 months ago)

I've never had any problems with honey. Lots of people use it. Makes nice thick LCs ready in about a week.


--------------------
rahz

comfort pleasure power love     truth awareness peace I am      I feel      I do     I love  I speak    I see    I know

"If you knew how quickly people forget the dead... you would stop living to impress people." — Christopher Walken

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