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shroomsisay
Stranger

Registered: 01/20/12
Posts: 713
Last seen: 9 years, 9 months
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Constantly stirred Liquid Culture ?
#16812644 - 09/08/12 05:39 PM (12 years, 4 months ago) |
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I am trying to make a liquid culture of penis envy. I am using extra light malt extract and it is being constantly stirred.
My question is with cubensis LC being constantly stirred should it turn milky or should I see mycelium floating in the LC. I have only made an LC of pan cam also constantly stirred and after a week I had about 2 inches of mycelium in the bottom if i would let it settle.
But with the penis envy cubensis it is only turning milky. I am not sure if this is the way it should be or if I got a bad syringe.
Any help would be greatly appreciated!!
-------------------- I thought I was wrong once but I was mistaken.
If your not living on the edge your taking up to much space!!
Edited by shroomsisay (09/08/12 05:41 PM)
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TheDeathryder
Reaper



Registered: 04/26/12
Posts: 2,636
Loc: Hell
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Re: Constantly stirred Liquid Culture ? [Re: shroomsisay]
#16812720 - 09/08/12 05:49 PM (12 years, 4 months ago) |
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If you're inoculating from spores, it's probably a bacterial contaminate. No spore print is 100% clean. Some people have had luck with LC from spores, but the majority have not.
I would get the spores started on agar, then make an LC from that
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shroomsisay
Stranger

Registered: 01/20/12
Posts: 713
Last seen: 9 years, 9 months
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Re: Constantly stirred Liquid Culture ? [Re: TheDeathryder]
#16812948 - 09/08/12 06:39 PM (12 years, 4 months ago) |
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It was started from multispore syringe.
-------------------- I thought I was wrong once but I was mistaken.
If your not living on the edge your taking up to much space!!
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Constantly stirred Liquid Culture ? [Re: shroomsisay]
#16813003 - 09/08/12 06:53 PM (12 years, 4 months ago) |
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well u need to shoot up a couple test jars and see if they fruit without contams. If they do then ur all good...if they contam, then ur a statistic.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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shroomsisay
Stranger

Registered: 01/20/12
Posts: 713
Last seen: 9 years, 9 months
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Re: Constantly stirred Liquid Culture ? [Re: PussyFart]
#16813030 - 09/08/12 06:59 PM (12 years, 4 months ago) |
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I forgot to mention... I inoculated some grain jars with the multispore syringe with no growth after 8 days. Then a few days later trich developed. I believe I got a bad syringe!
since I never got a cubensis LC going. I really want to know if I will get mycelium like I did with the pan cam or if it will just turn milky.
-------------------- I thought I was wrong once but I was mistaken.
If your not living on the edge your taking up to much space!!
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