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OfflinePsilobilly
Professor
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Registered: 07/03/12
Posts: 52
Last seen: 12 years, 4 months
First Attempt Spore Syringe Making Tonight
    #16804373 - 09/07/12 11:07 AM (12 years, 4 months ago)

I have lots of spore prints (more than 30)

I sterilised the aluminium foil with alcohol and evaporated under a hot grill to evaporate and ensure sterility.

I DO NOT have a glove box and my next project is to make one.

Do I try and scape the spores into a small jar of pressure cooked cooled water OR pour sterilised water over the prints then retract into a syringe.

Finally, why is it necessary to flush out a syringe several times with sterilised water if the syringes and needles are sterile as stated on the package? I have a large quantity of sterilised water left in the PC if this is necessary!

I have never suffered any contamination from my first attempt using a commercial spore syringe and if this contaminates I know I'll need a glove box. I will be wearing sterile gloves, I will bleach the working area and wear a surgical mask etc.


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"Steal a Little and They Throw You in Jail, Steal a Lot & They Make You King"

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OfflineHybridprX
Biodegrader of coir
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Registered: 01/29/08
Posts: 2,588
Loc: Canada Flag
Last seen: 7 years, 4 months
Re: First Attempt Spore Syringe Making Tonight [Re: Psilobilly]
    #16804466 - 09/07/12 11:20 AM (12 years, 4 months ago)

http://www.shroomery.org/forums/showflat.php/Number/16664885

Check it out op, will answer your questions.


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Offlinetugmyroo
The Mushroom Man
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Registered: 10/18/11
Posts: 74
Loc: British Columbia, Canada Flag
Last seen: 11 years, 3 months
Re: First Attempt Spore Syringe Making Tonight [Re: HybridprX]
    #16805098 - 09/07/12 01:29 PM (12 years, 4 months ago)

Thanks nice link. That helped me out with some stuff to

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