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Invisiblechopstick
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The fuck is this shit?
    #16798561 - 09/06/12 01:14 PM (12 years, 4 months ago)

Alright guys, I really need some help here.

I knocked up 30 jars, all Oak Ridge multispore. 1 got the Mean green so that leaves me with 29. At first, they were all colonizing beautifully, and rapidly. They all made it to about 30-40% colonization before I shook them, about a week post innoc.

Fast forward another week, my jars should be at full colonization right? Wrong. Some of them have made it to 100%, but I'd say about half have this weird looking contam in them. I used 2 60 cc syringes for these, and did another 75 or so jars after these, so now I'm worried they all will have it.

Pics:






I have never seen this contam before. It didn't show up until after the shake either, but now I have some other jars that you can clearly see weird dots in along with the myc that haven't been shaken yet. It is most visible on the sunflower seeds but you can also plainly see it on some milo and millet.

Anyone know what this shit is? A bunch of my jars have made it to 100% and this stuff is not visible, so I think I will go ahead and use those and see what happens. The rest will obviously have to be tossed...don't think I'm going to try to use 60cc syringes in this manner anymore. I used standard sterile procedure, water was boiled then added to syringe then prepped in a SAB.

Any thoughts are appreciated.

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Offlinetwistedty
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Re: The fuck is this shit? [Re: chopstick]
    #16798564 - 09/06/12 01:15 PM (12 years, 4 months ago)



dunno try contam forum

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Invisiblechopstick
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Re: The fuck is this shit? [Re: twistedty]
    #16798623 - 09/06/12 01:25 PM (12 years, 4 months ago)

ah shit your right, shoulda posted this in there.

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Offlinetwistedty
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Re: The fuck is this shit? [Re: chopstick]
    #16798630 - 09/06/12 01:26 PM (12 years, 4 months ago)

get faster answers at least :thumbup:

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InvisibleDynGBreeD
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Re: The fuck is this shit? [Re: chopstick]
    #16798635 - 09/06/12 01:28 PM (12 years, 4 months ago)

Looks super wet...

:awewtf:


--------------------

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Invisiblechopstick
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Re: The fuck is this shit? [Re: twistedty]
    #16798642 - 09/06/12 01:29 PM (12 years, 4 months ago)

Yea they may have been a bit overhydrated, I just started using these grains and kinda rushed em'. I don't think that's why though.

Edit: I should also add that the grains didn't look wet at all before the shake.

Edited by chopstick (09/06/12 01:33 PM)

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Offlinetwistedty
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Re: The fuck is this shit? [Re: DynGBreeD]
    #16798654 - 09/06/12 01:32 PM (12 years, 4 months ago)

Quote:

DynGBreeD said:
Looks super wet...

:awewtf:





thats what she said

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InvisibleTranscendingLife
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Re: The fuck is this shit? [Re: twistedty]
    #16798665 - 09/06/12 01:33 PM (12 years, 4 months ago)

Bacteria. Some yeast in there, it looks like, too.
Quote:

twistedty said:
Quote:

DynGBreeD said:
Looks super wet...

:awewtf:





thats what she said




^^this, too. :rofldrunk:


--------------------
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:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
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Offlinetripdawg420
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Re: The fuck is this shit? [Re: TranscendingLife]
    #16798687 - 09/06/12 01:38 PM (12 years, 4 months ago)

bacteria :whathesaid: :zombie3: sucks i feel your pain happens to me when i need shit the most it seems like :shrug:


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epic GT mono tub
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results :thumbup:

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Invisiblechopstick
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Re: The fuck is this shit? [Re: tripdawg420]
    #16798801 - 09/06/12 02:06 PM (12 years, 4 months ago)

I was prepping a large amount of grain at once, some of the grain I may have accidentally let boil too long (I was using TranscendingLife's tek.) since I wasn't around the pot to watch it vigilantly as I was multitasking at the time. Not all my jars look that wet, only a handful, and they looked fine before the shake..

However, I really don't think its the wetness that caused it. And I don't think that looks like any kind of standard bacteria either. It is pinkish/gray dots that resemble mycelium on the grain but is obv. some kind of contaminant.

I have a bunch of other jars that I did other strains of and prepped the grain in the same way, none of them are that wet OR have the weird dots in them, which really leads me to believe the contam was present in the syringes I used.

I am just confused as to what contam it could possibly be since Ive never encountered it before.

Edited by chopstick (09/06/12 02:07 PM)

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InvisibleTranscendingLife
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Re: The fuck is this shit? [Re: chopstick]
    #16798821 - 09/06/12 02:12 PM (12 years, 4 months ago)

The dots are yeast.
There's bacteria in there, too.

check out this sexy jar of yeast:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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Offlinetwistedty
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Re: The fuck is this shit? [Re: TranscendingLife]
    #16798826 - 09/06/12 02:13 PM (12 years, 4 months ago)

i wanna smell it

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Invisiblechopstick
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Re: The fuck is this shit? [Re: TranscendingLife]
    #16799004 - 09/06/12 02:49 PM (12 years, 4 months ago)

Damn :frown:

thanks for the response!

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Offlinetugmyroo
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Re: The fuck is this shit? [Re: chopstick]
    #16799101 - 09/06/12 03:09 PM (12 years, 4 months ago)

Quote:

i wanna smell it



nasty man why ?:laugh:

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