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OfflineJostVanDyke
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Probably a dumb idea, but and idea indeed.
    #16798468 - 09/06/12 12:55 PM (12 years, 4 months ago)

Alot of people have issues with contamination when using WBS. I was thinking this... (and before you say omg this is so dumb or whatever else, this is strictly just an idea and NOT what I actually do)

Soak grains for 24 hours.
Jar grains.
LEt grains set for 4-5 days until some type of contam shows up on jars.
PC for 90 minutes.


Wouldn't this allow for any endospores and other contams to build up and then kill them in 1 fell swoop?

Just an idea.

Also for those of you who have alot of contams, I figured out 1 thing that helps alot, and that is to let jars sit out for a few days to detect if pressure cooking was done correctly, THEN inoculating them, so that you're not wasting ms/lc or whatever else on already contaminated jars.

What do you guys think?!


--------------------
-JVD

I'll do anything I can to help anyone. I have a little over 2 years of growing experience so if you have a question on anything just shoot me a PM and i'll do my best to help you.

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Offlinetwistedty
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Re: Probably a dumb idea, but and idea indeed. [Re: JostVanDyke]
    #16798497 - 09/06/12 01:01 PM (12 years, 4 months ago)

no


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Offlinecloudpersona
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Re: Probably a dumb idea, but and idea indeed. [Re: JostVanDyke]
    #16798524 - 09/06/12 01:05 PM (12 years, 4 months ago)

Quote:

JostVanDyke said:
Alot of people have issues with contamination when using WBS. I was thinking this... (and before you say omg this is so dumb or whatever else, this is strictly just an idea and NOT what I actually do)

Soak grains for 24 hours.
Jar grains.
LEt grains set for 4-5 days until some type of contam shows up on jars.
PC for 90 minutes.


Wouldn't this allow for any endospores and other contams to build up and then kill them in 1 fell swoop?

Just an idea.

Also for those of you who have alot of contams, I figured out 1 thing that helps alot, and that is to let jars sit out for a few days to detect if pressure cooking was done correctly, THEN inoculating them, so that you're not wasting ms/lc or whatever else on already contaminated jars.

What do you guys think?!



No, then it would produce an enormous amount of endospores instead of the few that were already present.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
– Terence McKenna
“If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.”
-Terence McKenna

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Invisiblesnakeinthegrass
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Re: Probably a dumb idea, but and idea indeed. [Re: twistedty]
    #16798535 - 09/06/12 01:07 PM (12 years, 4 months ago)

Quote:

twistedty said:
no






i scrolled down to see this.... soda came out my nose... :rofl:

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Offlinetwistedty
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Re: Probably a dumb idea, but and idea indeed. [Re: snakeinthegrass]
    #16798560 - 09/06/12 01:14 PM (12 years, 4 months ago)

endospores are always present you just slow them enough to give the mycelium chance to win the sub over first. 

something like that.

RR said it but i cant find the quote

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OfflineJostVanDyke
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Registered: 09/01/12
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Re: Probably a dumb idea, but and idea indeed. [Re: twistedty]
    #16798640 - 09/06/12 01:29 PM (12 years, 4 months ago)

I figured it would be a bad idea, just thought I would ask. Makes sense that it would work though using Tyndallization which is heating to 250 degrees for 15 minutes each day for 3 days. This is supposed to kill all of the endospores.

BTW, It doesn't "produce" any endospores, they just come out of hibernation when they are introduced to favorable conditions. They are already there. Which is the reason why I asked, because if you can bring them out of hibernation then you can kill them completely, and have none remain after repeating the process a few times. This could be done to grain, but not substrate as substrate is only pasteurized and not sterilized to the temperatures needed to kill the bacteria completely.

Sources:
Endospore Wiki
Tyndallization Wiki

Check in to it.

I will say again, that I am not trying to say this is some great tek or a good idea, just a topic for debate.


--------------------
-JVD

I'll do anything I can to help anyone. I have a little over 2 years of growing experience so if you have a question on anything just shoot me a PM and i'll do my best to help you.

Edited by JostVanDyke (09/06/12 01:38 PM)

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