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AcidTG


Registered: 08/03/09
Posts: 359
Last seen: 12 years, 2 months
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Sterilizing everything *DELETED*
#16796986 - 09/06/12 05:53 AM (12 years, 4 months ago) |
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Post deleted by AcidTGReason for deletion: .
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HybridprX
Biodegrader of coir



Registered: 01/29/08
Posts: 2,588
Loc: Canada
Last seen: 7 years, 4 months
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Re: Sterilizing everything [Re: AcidTG]
#16797013 - 09/06/12 06:03 AM (12 years, 4 months ago) |
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Its your carpet....
I had a apartment back in the day that had wall to wall carpeting, all healthy cultures would turn green....heavy fabrication is extremely bad in the cultivation environment.
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AcidTG


Registered: 08/03/09
Posts: 359
Last seen: 12 years, 2 months
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Re: Sterilizing everything *DELETED* [Re: HybridprX]
#16797084 - 09/06/12 06:14 AM (12 years, 4 months ago) |
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Post deleted by AcidTGReason for deletion: .
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HybridprX
Biodegrader of coir



Registered: 01/29/08
Posts: 2,588
Loc: Canada
Last seen: 7 years, 4 months
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Re: Sterilizing everything [Re: AcidTG]
#16797120 - 09/06/12 06:20 AM (12 years, 4 months ago) |
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A high efficiency carpet steam cleaner and U.V.C germicidal light were the two I used.....
If carpet is infested with trich though, it won't help...
Last time I went apartment hunting I made it a goal of mine for it to have hardwood floors after my experience with cultivation and wall to wall carpeting.
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donrjuan
On the Grind


Registered: 10/20/10
Posts: 534
Loc: State of Boredom
Last seen: 5 months, 14 days
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Re: Sterilizing everything [Re: AcidTG]
#16797147 - 09/06/12 06:33 AM (12 years, 4 months ago) |
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I put down heavy rubber exercise mats in my grow room and "clean" room, both are carpeted. I believe this helped me out. Do not use drop plastic to line your floors as one mistake with your alcohol lamp and you have a fire that is almost impossible to stop.
Are you using a glove box minimum? If so then clean everything (GB, tables, etc.) and load your clean PF jars and syringe into the clean box. I have 3" holes in my GB that can be plugged with two 3" threaded PVC pipe caps "sealing" the box. As you exit the room, spray plain tap water into the air using a mist bottle. This will knock down the majority of the contaminant in the air. Let this settle for a while and then go in and do your work. I throw up another light mist as I walk in to sit down at my station. This technique work great for me. Some also use a bleach solution to spray into the air but I ruined some scrubs this way and just went to tap water.
Also, your jars should not contaminate if sealed with proper filters even when placed directly next to a source of contamination. Good Luck!
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Grumpyorc
Ghost


Registered: 09/14/09
Posts: 2,041
Last seen: 5 years, 3 months
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Re: Sterilizing everything [Re: donrjuan]
#16797260 - 09/06/12 07:26 AM (12 years, 4 months ago) |
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i do my work in the kitchen, which is carpeted, next to the trashcan, with my dog running around the room.
do you turn your AC off before you start?
also theres no way contams like that are getting past your filter lids. i say somethings up with your procedure or with your genetic material. maybe im wrong good luck
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AcidTG


Registered: 08/03/09
Posts: 359
Last seen: 12 years, 2 months
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Re: Sterilizing everything [Re: AcidTG]
#16797777 - 09/06/12 10:26 AM (12 years, 4 months ago) |
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I am using EZ felt ghetto rigged filters. This jar contam has been a statistical anomaly though.
I am trying to soak these carpets with as much disinfectant chemicals as possible. I spray my house with highly concentrated quats weekly. I spray lysol aerosol on everything that I touch. I sterilize my hands when I walk in the door.
When I inoculate, or open my life, i make sure all airflow is shut off for as much still air a possible. I wear mask and gloves when doing work.
Basically, my house is just infested with spores. Should I fumigate with lysol, or is there a better airborne alternative?
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Grumpyorc
Ghost


Registered: 09/14/09
Posts: 2,041
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Re: Sterilizing everything [Re: AcidTG]
#16797794 - 09/06/12 10:30 AM (12 years, 4 months ago) |
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Quote:
AcidTG said: I am using EZ felt ghetto rigged filters.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 4 years, 5 months
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Re: Sterilizing everything [Re: AcidTG]
#16798025 - 09/06/12 11:26 AM (12 years, 4 months ago) |
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Quote:
AcidTG said:
My sterile procedures are good. I flame sterilize my needle, wipe everything down with alcohol, including the ports and needle before/after flaming it.
wiping needle with alcohol after flame sterilize is counter productive
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
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Re: Sterilizing everything [Re: twistedty]
#16798038 - 09/06/12 11:27 AM (12 years, 4 months ago) |
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Quote:
RogerRabbit said: Wiping a loop with alcohol after flame sterilizing is like wiping shit on your face after taking a shower. RR
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Grumpyorc
Ghost


Registered: 09/14/09
Posts: 2,041
Last seen: 5 years, 3 months
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Re: Sterilizing everything [Re: twistedty]
#16798125 - 09/06/12 11:46 AM (12 years, 4 months ago) |
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But that's not why he failed
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vlajce92
Thinker


Registered: 12/24/10
Posts: 78
Last seen: 9 years, 11 months
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Re: Sterilizing everything [Re: twistedty]
#16798156 - 09/06/12 11:52 AM (12 years, 4 months ago) |
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Can you do that in Baathroom? Its nice if it has a little space, easier to prevent contaminations.. then in the room.
and all you have to do is to spray it with those substances for cleaning the air in rooms, let it stays for couple of minutes *30min best, and enter in there like a doctor in a surgery room.
-------------------- Tryed to cultivate in this...
...won!
Whats your favorite dish? im not gonna cook it but ill order it from ZANZIBAR <3
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
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Re: Sterilizing everything [Re: Grumpyorc]
#16798215 - 09/06/12 12:04 PM (12 years, 4 months ago) |
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Quote:
Grumpyorc said: But that's not why he failed
true, just saying
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donrjuan
On the Grind


Registered: 10/20/10
Posts: 534
Loc: State of Boredom
Last seen: 5 months, 14 days
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Re: Sterilizing everything [Re: AcidTG]
#16803447 - 09/07/12 06:32 AM (12 years, 4 months ago) |
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Stop with the felt. You may as well put a window screen over the jar. Go to a big box store an buy a Tyvek paint suit and use that material to make filters. Consider this the "ghetto" minimum you where looking to bottom out at.
So you are not using a glove box? Spend $8 at the same BB store and get a medium tote. Toss the lid and cut 2 holes for your hand near what is now the top. Flip the tote over on a table and wallah! Instant GB. If you use the knock down technique I disclosed above with plain tap water and use the GB you will see an improvement. Shoot for more than the minimum. Good luck!
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cozc
Stranger


Registered: 06/14/11
Posts: 380
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Re: Sterilizing everything [Re: donrjuan]
#16803470 - 09/07/12 06:44 AM (12 years, 4 months ago) |
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Quote:
donrjuan said: Stop with the felt. You may as well put a window screen over the jar. Go to a big box store an buy a Tyvek paint suit and use that material to make filters. Consider this the "ghetto" minimum you where looking to bottom out at.
So you are not using a glove box? Spend $8 at the same BB store and get a medium tote. Toss the lid and cut 2 holes for your hand near what is now the top. Flip the tote over on a table and wallah! Instant GB. If you use the knock down technique I disclosed above with plain tap water and use the GB you will see an improvement. Shoot for more than the minimum. Good luck!
I've been using ez-felt discs on the outside of my jars for some time now and have had zero contams. I even knocked through the filter and left a nice hole over my injection port and they still worked fine. They usually last for like 3-4 pc runs
Filters aren't that hard I've used envelope tyvek only on 100+ jars, no worries. I've used ez-felt on over 100 jars, no worries. I've just poked one whole in the lid and stuffed with polyfil and knocked through it and every thing, no worries. Hell I've even made an LC and grain jars that had one 1/4" hole that i just put micro pore tape over and they all lasted fine (even tested the lc)
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<--- good day
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donrjuan
On the Grind


Registered: 10/20/10
Posts: 534
Loc: State of Boredom
Last seen: 5 months, 14 days
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Re: Sterilizing everything [Re: cozc]
#16803490 - 09/07/12 06:52 AM (12 years, 4 months ago) |
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Polyfill and Tyvek have both worked very well for me. Felt was always terrible. I tried thin, thick, colored, etc. I layered it 2 thick to where the lid barely fit. All failed or under performed in controlled experiments with my synthetic filter discs. These are my observations only but I have read other's accounts as well.
And you are correct, filters are simple and that is not the issue here. The OP just needs to work on sterile procedures.
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x CiVil x

Registered: 07/05/12
Posts: 634
Loc: Flying With Mother Nature
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Re: Sterilizing everything [Re: donrjuan]
#16803499 - 09/07/12 06:56 AM (12 years, 4 months ago) |
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I have a filter tek that work's in my journal. Super cheap.
-------------------- “Empty your mind, be formless, shapeless - like water. Now you put water into a cup, it becomes the cup, you put water into a bottle, it becomes the bottle, you put it in a teapot, it becomes the teapot. Now water can flow or it can crash. Be water, my friend.” Bruce Lee
myco-tek.org
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donrjuan
On the Grind


Registered: 10/20/10
Posts: 534
Loc: State of Boredom
Last seen: 5 months, 14 days
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Re: Sterilizing everything [Re: x CiVil x]
#16803504 - 09/07/12 06:58 AM (12 years, 4 months ago) |
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I think the link is broken.
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x CiVil x

Registered: 07/05/12
Posts: 634
Loc: Flying With Mother Nature
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Re: Sterilizing everything [Re: donrjuan]
#16803514 - 09/07/12 07:01 AM (12 years, 4 months ago) |
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It's in my journal. I don't know how that's possible. lol 
Click on the book under my shrooms.
I honestly think you have bad spawn OP. How did you sterilize your grain? What did you inoculate with?
-------------------- “Empty your mind, be formless, shapeless - like water. Now you put water into a cup, it becomes the cup, you put water into a bottle, it becomes the bottle, you put it in a teapot, it becomes the teapot. Now water can flow or it can crash. Be water, my friend.” Bruce Lee
myco-tek.org
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donrjuan
On the Grind


Registered: 10/20/10
Posts: 534
Loc: State of Boredom
Last seen: 5 months, 14 days
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Re: Sterilizing everything [Re: x CiVil x]
#16803518 - 09/07/12 07:02 AM (12 years, 4 months ago) |
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My bad! Just got up and thoght you where directing us to the signature.
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Grumpyorc
Ghost


Registered: 09/14/09
Posts: 2,041
Last seen: 5 years, 3 months
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Re: Sterilizing everything [Re: cozc]
#16803637 - 09/07/12 07:53 AM (12 years, 4 months ago) |
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Quote:
cozc said:
Quote:
donrjuan said: Stop with the felt. You may as well put a window screen over the jar. Go to a big box store an buy a Tyvek paint suit and use that material to make filters. Consider this the "ghetto" minimum you where looking to bottom out at.
So you are not using a glove box? Spend $8 at the same BB store and get a medium tote. Toss the lid and cut 2 holes for your hand near what is now the top. Flip the tote over on a table and wallah! Instant GB. If you use the knock down technique I disclosed above with plain tap water and use the GB you will see an improvement. Shoot for more than the minimum. Good luck!
I've been using ez-felt discs on the outside of my jars for some time now and have had zero contams. I even knocked through the filter and left a nice hole over my injection port and they still worked fine. They usually last for like 3-4 pc runs
Filters aren't that hard I've used envelope tyvek only on 100+ jars, no worries. I've used ez-felt on over 100 jars, no worries. I've just poked one whole in the lid and stuffed with polyfil and knocked through it and every thing, no worries. Hell I've even made an LC and grain jars that had one 1/4" hole that i just put micro pore tape over and they all lasted fine (even tested the lc)
Incorrect
you are dealing with a hige contaminate outbreak right now
your filters are the obvious culpret. i said that like 10 posts ago and everyone agrees. since there is no logical, alternative, hypothesis (and its obvious) your next step is to buck up and buy a 3 dollar bag of polyfil at walmart. noone reports failure with polyfil. including me
or filter disks!! iv used them too and they are just as awesome. i think they even look better but polyfil is faster to obtain
also tyvek from envelopes is not reccomended becasue it is crap, its too thin. buy a pain suit
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cozc
Stranger


Registered: 06/14/11
Posts: 380
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Re: Sterilizing everything [Re: Grumpyorc]
#16803716 - 09/07/12 08:33 AM (12 years, 4 months ago) |
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I think you can get the wrong type of ezfelt cause one time I went to a diff craft store and they had "easy felt" not "ez-felt" and that entire round went south, even from a tested glc that performed fine on other jars afterward
Wish I had the extra 300$ to order a proper filter disk for every one of my jars lmao
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<--- good day
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HybridprX
Biodegrader of coir



Registered: 01/29/08
Posts: 2,588
Loc: Canada
Last seen: 7 years, 4 months
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Re: Sterilizing everything [Re: cozc]
#16804422 - 09/07/12 11:14 AM (12 years, 4 months ago) |
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Getting the filter wet is not the issue here (Although it can be)
Op's problems are coming from poor inoculation procedures, not the filter.
The filter will always come out damp or wet from steam vapors being released from within the jar, you cannot prevent this!
Leave the foil on the jar until you are ready to use it and then take a paper towel and just gently dab the filter material to absorb the excess water...with polyfill it will evaporate fairly quick (within a day) same with tyvek, but micropore tape needs to be dabbed and a second piece put overtop. Works just fine for me.

If it was the filter, i'd be having the same problem.

Build a glovebox op.
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