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Offlinezepski
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Registered: 10/01/11
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Contam woes...Sad week
    #16796731 - 09/06/12 02:22 AM (12 years, 4 months ago)

Well week has been a sad week.  I just back recently from a work trip to a very sad sight.  In this week I've lost 3 new tubs to trich obviously from bad grain spawn since it was before the 1st flush.  However since the loss of these tubs All my other tubs (4) have fall to the green monster.  I didn't open any of the tubs in the grow area of course but obviously this didn't help.  I even went through the effort to shower and put on new tubs after the removal of each tub since the contamed 1 after another.  I caught all but 1 before they turned green.  Given these tubs were on their 3rd flush its still saddens me.

I guess I'm writing this for 1 to show even if you have a bunch of grows under your belt that you can still lose everything in a flash for stupid reasons. 

The thing that really pisses me off is that I saw nothing wrong with any of the grain jars.  The only reason i blame the spawn is that I followed the normal routine only difference is that rather then just adding my worm casting in the bucket with the coir as normal is that i actually pasteurized the WC separately from the coir this time.  Also each batch of substrate was made separately so the chances that the substrate was the problem seems to be minimal compared to the spawn being the issue.

As always I am always open to suggestions or comments where I could of went wrong. :smile: I mean its always nice to have a fresh set of eyes on the situation.  Also sorry for the long post.  Its late so i tend to rant hehehe. :grin: 

But tomorrow is another day and already got more rye soaking.  Along with a few jars already colonized and ready for the next g2g.  I think I also may make some agar but we will see how the day goes.

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Offlinetwistedty
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Re: Contam woes...Sad week [Re: zepski]
    #16796819 - 09/06/12 03:34 AM (12 years, 4 months ago)

shit man sucks i havent dealt with massive contam breakout yet.  i know its possible just hope you zero out on what is causing this

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Offlinemindraped
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Re: Contam woes...Sad week [Re: twistedty]
    #16796837 - 09/06/12 03:52 AM (12 years, 4 months ago)

I've had the same thing happen along with 20 jars that I inoculated gtg. What's your inoculation procedure?

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OfflineWing
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Re: Contam woes...Sad week [Re: mindraped]
    #16797535 - 09/06/12 09:13 AM (12 years, 4 months ago)

I've had trich a couple times in the last year. It sux man :sad:

Luckily it's only been with a PEU syringe that I've done MS inoculations with to find a good clone candidate. If I had ruined any of the tubs I routinely do, I would have been upset.


--------------------
My Old Grow Logs


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Offlinezepski
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Re: Contam woes...Sad week [Re: mindraped]
    #16797821 - 09/06/12 10:37 AM (12 years, 4 months ago)

Quote:

mindraped said:
I've had the same thing happen along with 20 jars that I inoculated gtg. What's your inoculation procedure?




The jars that were used in the 3 original bad tubs came from a clone->agar->grain then g2g and this was about the 6th time g2g was done.  So i assume it was just my last transfer was bad.

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