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battypie
That guy



Registered: 08/10/12
Posts: 116
Last seen: 10 years, 4 days
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These look fine?
#16784492 - 09/03/12 10:52 PM (12 years, 4 months ago) |
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So my friend wanted to try growing some mushrooms for fun. He started with a king oyster spore syringe to knock up some brf jars. I'd say the vermiculite is more medium/fine than anything else. Standard pf tek for these since they were the first set of jars. Pressure cooked for 90 minutes and left in the pc to cool over night. Foil lids were used for the lack of effort put towards the first attempt. Jars were knocked up on the kitchen counter. Everything was wiped down with iso, and all fans were off in the room. Gloves were put on and sterilized with some more iso. Needle was flame sterilized to red hot then cooled with an iso soaked paper towel. The needle was sterilized between each jar too. After 4 jars were done since that was all that fit in the pc at a time the foil lids were put back on, both layers (punctured layer and solid layer). They were then put in the closet which stays between 80-84F. After reading around I told him he should remove the top layer of foil so there would be GE for the cakes. While removing the foil some tore. He paid no mind since the verm is the contam barrier anyway. After a while though very fluffy white stuff showed up. That isn't cobweb mold is it? Or is it just mycellium growing through? There is a thin spot on the barrier verm and he is concerned of contams getting through.
Also, some jars are near 100% colonization. There appears to be excess water at the bottom of the jars and some yellow spots on the cakes. Is that just myc piss or is it a contam? 
Also he made a LC. It was made with about 3.5% honey to water ratio. The honey he used was honey he collected from a bee hive on the property he visited in Florida. It was pressure cooked with the band loose for 15 minutes. When the 15 was up he cut the gas immediately and let it cool until the pressure lock went down. Once that happened he opened the pc and screwed the band down tight and let the jar cool overnight. To break the vacuum he used an airport sterilized with the LC in the pc. The color came out kind of a piss yellow but when held up to a fluorescent light it looks pretty clear. The jar was wiped down with iso. The same sterile procedures for the pf cakes was used here. Anyway, it has been about 5 or 6 days since the spores were introduced and there seems to be health growth. What seems to be the mycellium is all sinking to the bottom. Is this normal? The LC is agitated daily. There is a stir bar in there but he hasn't finished his stir plate yet. Had to add some final adjustments to it before it can be used.    I hope sufficient information has been provided for people to help
Edited by battypie (09/03/12 11:42 PM)
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Lightning Turd
Stranger
Registered: 09/03/12
Posts: 1
Last seen: 12 years, 4 months
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Re: These look fine? [Re: battypie]
#16784554 - 09/03/12 11:09 PM (12 years, 4 months ago) |
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count on the turd to solve this mystery.
verm barrier on the first one is growing contaminated. looks like shit to the turd (and trust me, the turd knows a turd when he sees one). 2nd one looks normal as far as metabolites go. lc's are alright as far as looks go, but you gotta put em to the test before anyone will truly know.
Edited by Lightning Turd (09/03/12 11:15 PM)
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battypie
That guy



Registered: 08/10/12
Posts: 116
Last seen: 10 years, 4 days
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I kinda figured it was a contam from everything I had read. Can he just dump off the verm and hope its safe or just trash the whole thing and boil the jar? He plans on getting some brf cakes made up in the next couple days to test the lc on before a bulk grow is done with it. Just wanted a third opinion on if they looked healthy and normal as far as looks go.
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battypie
That guy



Registered: 08/10/12
Posts: 116
Last seen: 10 years, 4 days
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Re: These look fine? [Re: battypie]
#16784671 - 09/03/12 11:57 PM (12 years, 4 months ago) |
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Because of that one jar I had him check all of his other jars. All of the other jars show the same growth, and actually, even more!
That seems too thick to be cobweb mold. Or can cobweb mold get that thick? There are drops of condensation on the top of it too.

This one looks like it came from the myc
This one is starting to have growth but it looks like its coming from the myc
Keep in mind this is king oyster mycelium. Not any type of cube. Doesn't oyster mushrooms myc look more whispy and grow more aggressively too?
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DynGBreeD
┌∩┐(◣_◢)┌∩┐

Registered: 01/15/11
Posts: 3,639
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Re: These look fine? [Re: battypie]
#16786598 - 09/04/12 12:31 PM (12 years, 4 months ago) |
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Your BRF all look to be 
You can get rid of cobweb with a H2o2/Water Mixture (Search Engine) If that is the only contaminate it has.. For any other, I wouldn't of opened them indoors. Take them outside and bury them in some mulch, water it down. Pray for outdoor fruits.
EDIT: was foil your only means for a lid? Or were there actual Mason lids involved? If there weren't, you should of NEVER removed the foil. GE would come from the 4 inoculation holes you made.
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Edited by DynGBreeD (09/04/12 12:33 PM)
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battypie
That guy



Registered: 08/10/12
Posts: 116
Last seen: 10 years, 4 days
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Re: These look fine? [Re: DynGBreeD]
#16787312 - 09/04/12 02:48 PM (12 years, 4 months ago) |
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A layer of foil with the inoculation holes was left on the whole time. That appeared to be the only contamination but they have been taken out back and buried. You win some you lose some. All of the current jars use mason lids with micropore tape over the inoculation holes.
EDIT The jars have been filled half way with h202 and swirled it around the inside of them. They are now sitting in boiling water and will be for maybe 60 min if that sounds like an appropriate amount of time. The grow room (walk in closet) never had moving air in it since it was a closet. All the walls were still sprayed with iso and wiped down in hopes that it would help in case spores got anywhere. There is now a hepa filter running in there to try to scrub the air a bit also. Does all of this sound suitable for sterile procedures to help reduce future issues? Hands were washed between each step and cleaned with h2o2 and iso. Seems like overkill but I've read things where people let one jar open and it could ruin all future attemps. Oh, and all of the other jars that were colonizing were removed from the room and their surfaces were wiped down with iso.
Edited by battypie (09/04/12 02:54 PM)
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