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un-known-ome
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Registered: 03/04/12
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Multispore to Rye Problem
#16783619 - 09/03/12 07:52 PM (12 years, 4 months ago) |
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From the same batch of rye berries, I prepared six identical substrate jars. Three of the jars were inoculated with an LC and are flourishing. The other three jars were inoculated with freshly made spore syringes. None of the three jars are showing any signs of growth or contamination whatsoever. It has been at least two weeks since inoculation. Although I have never used rye berries before, I find this curious. I have successfully made spore syringes before and successfully inoculated and colonized BRF jars. Why might this be?
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snakeinthegrass




Registered: 08/12/12
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how long did you leave the syringes sit before you inoculated?
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un-known-ome
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Quote:
snakeinthegrass said: how long did you leave the syringes sit before you inoculated?
Not long at all, but I've made syringes before and inoculated successfully without a proper "hydration" window. The syringes were made from fresh prints. Also I believe I inoculated a rye jar with another syringe and similarly nothing happened.
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TheDeathryder
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Did you shake the jars after injecting multispore solution? How many cc's did you inject? Did you shake the syringe?
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JostVanDyke
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Has to be something with your spore syringe. Possibly try to check out a drop of it under your microscope if you have one or try to see what it looks like on agar. Otherwise I don't know what to say other than possibly to test again with a pint or so of newly pc'd grain.
IMO something probably has gone wrong with the syringe, that or maybe they are just taking a bit longer to show growth.
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Edited by JostVanDyke (09/03/12 08:43 PM)
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Meno
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Re: Multispore to Rye Problem [Re: JostVanDyke]
#16785283 - 09/04/12 05:18 AM (12 years, 4 months ago) |
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I've had rye injected with SS that took over 20 days to show colonization, there is a reason LC is preferred over SS.
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PussyFart
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Re: Multispore to Rye Problem [Re: Meno]
#16785289 - 09/04/12 05:20 AM (12 years, 4 months ago) |
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Quote:
Meno said: I've had rye injected with SS that took over 20 days to show colonization, there is a reason LC is preferred over SS.
Its not
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HybridprX
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Re: Multispore to Rye Problem [Re: PussyFart]
#16785301 - 09/04/12 05:29 AM (12 years, 4 months ago) |
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Heh, I always have the same problem op, I make my own syringes and regardless I always get a few jars that fail to germinate anything, even mold and bacteria...
About two and a half weeks ago I inoculated 8 jars of wbs, 4 of them germinated, three didnt do anything and 1 got wet spot due to access spore solution (4.5ccs was experimenting)
I took the first completed jar and inoculated the three jars that failed to germinate via g2g from the first completed jar, they were done in about 5-7 days...
So, moral of the story. If you fail to see any growth (Mycellium, mold, bacteria) you can always attempt to re-inoculate.
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PussyFart
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Re: Multispore to Rye Problem [Re: HybridprX]
#16785312 - 09/04/12 05:32 AM (12 years, 4 months ago) |
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Quote:
HybridprX said:
So, moral of the story. If you fail to see any growth (Mycellium, mold, bacteria) you can always attempt to re-inoculate.
Again, bad advice, dont re-inoculate. Start over. If the jar didnt colonize the first time, why would it the 2nd time? It didnt colonize for a reason...
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
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HybridprX
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Re: Multispore to Rye Problem [Re: PussyFart]
#16785331 - 09/04/12 05:38 AM (12 years, 4 months ago) |
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huh?
A properly prepared grain jar, that has been inoculated with a properly prepared syringe will still be good if the spores fail to germinate.
If they've been sitting around for two weeks and nothing is happening (Slimy grains/mold) and shake just like they came out of the pc, they're still good to use.
If not, the cultivator needs to spend some more time fine tuning their sterilization/inoculation procedures.
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PussyFart
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Re: Multispore to Rye Problem [Re: HybridprX]
#16785339 - 09/04/12 05:42 AM (12 years, 4 months ago) |
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You wont see one trusted cultivator here that would suggest to re-inoculate. Why is that?
Just do a search for reinnoculate....
Quote:
HybridprX said: A properly prepared grain jar, that has been inoculated with a properly prepared syringe will still be good if the spores fail to germinate.
Yea if the moisture content isnt off, or if there is no hidden contams....but if this werent the case...wouldnt the first inoculation have worked? Considering the syringe has worked on other jars just fine.
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Edited by PussyFart (09/04/12 05:44 AM)
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un-known-ome
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Re: Multispore to Rye Problem [Re: PussyFart]
#16785526 - 09/04/12 08:00 AM (12 years, 4 months ago) |
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I cannot say for sure what the problem is, but my inoculation technique is fine and am confident that every step was done properly. Or perhaps I didn't prepare my rye properly and the LC's are still able to take off but the multispore solution cannot? It's a thought. I actually will go ahead and reinoculate because I have plenty of syringes and plenty of rye even if it's not recommended. Nothing to lose really if I'm going to toss the jars anyway.
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PussyFart
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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un-known-ome
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Re: Multispore to Rye Problem [Re: PussyFart]
#16785870 - 09/04/12 10:01 AM (12 years, 4 months ago) |
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Quote:
Notahacker420 said:

Hey come on now I have spores a plenty. It might be doomed to fail but if it somehow works then it will have been worth it. Unless someone else can say for sure why I'm not seeing any colonization?
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PussyFart
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BECAUSE THE MOISTURE LEVEL IS WRONG or BECAUSE OF A HIDDEN CONTAM!!!!
Waste ur spores i dont care, but if 1 or 2 jars goin bad is that important to u, i dont see u getting far in this hobby. Let it go and start over, why risk another fail?
It didnt colonize for a reason, duh!
But just add more spores, that should help, just fill the jar!
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
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Edited by PussyFart (09/04/12 10:19 AM)
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HybridprX
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Re: Multispore to Rye Problem [Re: PussyFart]
#16786046 - 09/04/12 10:38 AM (12 years, 4 months ago) |
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Wow.... lol
un-known-ume, There's no such thing as a hidden contam, either you're going to see mold somewhere in the jar or the grains will be slimy when moved side to side.
If the grain jars are sterilized properly, If your spore syringe was clean and your inoculation procedure is proper then there is no reason for bacteria/mold to be present in the jar. The moisture content should not be offset either.
Throwing things out that dont need to be thrown out is very wasteful, if it was contaminated, sure.
And like I said, the only type of "hidden" contaminate is bacteria and to eliminate that all you need to do is shake the jar side to side, crack the lid abit and smell, if it smells like grains, you're good to re-use.
Spores fail to germinate all the time even though I make and use the syringes within 72 hours, I always get a few stubborn jars.
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PussyFart
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Re: Multispore to Rye Problem [Re: HybridprX]
#16786052 - 09/04/12 10:39 AM (12 years, 4 months ago) |
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Quote:
HybridprX said: Wow.... lol
un-known-ume, There's no such thing as a hidden contam
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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TheDeathryder
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Re: Multispore to Rye Problem [Re: PussyFart]
#16786091 - 09/04/12 10:50 AM (12 years, 4 months ago) |
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Looks like it's time to break out the raincoats in this 
If the op wants to go ahead and try re-inoculating them, that's their choice. Once advice/opinion have been given, we've done our part.
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HybridprX
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This is far from a shit storm..... no ones even posted a picture of a gun threatening to shoot me yet over whos giving better advice, lol.
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cloudpersona
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Re: Multispore to Rye Problem [Re: HybridprX]
#16786106 - 09/04/12 10:54 AM (12 years, 4 months ago) |
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Quote:
HybridprX said: If your spore syringe was clean
No spore syringe is 100% clean, because no spore print is 100% clean.
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