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InvisibleBrain Fart
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Registered: 12/19/07
Posts: 2,538
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Re: Water bottle LC [Re: Hellyen]
    #16778341 - 09/02/12 07:42 PM (12 years, 4 months ago)

Quote:

DynGBreeD said:
Is this a Tek?
I'm assuming to replace a Glove Box..

Because I always do open air inoculation without contaminates.
But that doesn't mean its a good idea, or that it's proven to work.

Just wanted to add that what works for one or "some" won't work
for all. A lot of times you get Beginners looking to cut corners,
finding these Tek's.
Then Using them, end up with failed results. Then come and ask "why"
it failed....

In all actuality a hot oven creates air movement into the oven.(cold, high density air
always flows towards warm, low density air) the expansion of the hot air causes turbulence,
which increases the air movement around your sterile work area. Which is what you don't want...

You would be better using a Box with a Suran Wrap window.





Sorry dude, he's right. Oven tek is way outdated and actually more bad then good. RR will chime in and say so also.. that's why everyone is giving you so much heat. Simplefarmer is a stinky troll but in all actuality your oven tek thing is way way outdated and is actually advised against in several posts by trusted cultivators. Posting a tek about using this tek was sure to invoke flaming, surely you knew that?


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OfflineSimpleFarmer
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Registered: 04/04/12
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Re: Water bottle LC [Re: Brain Fart]
    #16778352 - 09/02/12 07:44 PM (12 years, 4 months ago)

Quote:

Simplefarmer is a stinky troll but


:dumbass:

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InvisibleBrain Fart
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Re: Water bottle LC [Re: SimpleFarmer]
    #16778387 - 09/02/12 07:52 PM (12 years, 4 months ago)

Quote:

SimpleFarmer said:
Quote:

Simplefarmer is a stinky troll but


:dumbass:





:trollmove:  :winning1:


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OfflineHellyen
Just Strange.
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Re: Water bottle LC [Re: SimpleFarmer]
    #16778410 - 09/02/12 07:56 PM (12 years, 4 months ago)

This is the last time i'll respond to this in the thread. I dont give a fuck about oven door tech! Its just how i chose to do mine. I understand you suck simple farmer. But apply tech in any clean working space and it = success. Have the guts to try it before you discount it, or just stfu and move on.


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I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

Edited by Hellyen (09/02/12 08:02 PM)

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OfflineSimpleFarmer
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Re: Water bottle LC [Re: Hellyen]
    #16778416 - 09/02/12 07:58 PM (12 years, 4 months ago)

:obamafrown:

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InvisibleKhii Khwaay
black tooth grin

Registered: 04/16/12
Posts: 2,277
Re: Water bottle LC [Re: SimpleFarmer]
    #16778500 - 09/02/12 08:21 PM (12 years, 4 months ago)

Ignoring the OvenDoor tek for a second... OP, how did you sterilize your medium? It seems to me that you just mixed honey and water and then inoculated.

I understand that you believe that bottled water is sterile, but dude, honey is FILTHY. It comes from bees and is exposed to all sorts of airborne contaminates before it even goes in the bottle.

Did you PC/Steam the bottle before you shot it up? And if you did, how did you keep it from becoming a lump in the bottom of the pot?

:shrug:

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OfflineHellyen
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Re: Water bottle LC [Re: Brain Fart]
    #16778541 - 09/02/12 08:33 PM (12 years, 4 months ago)

Yes Brain Fart (lol @ ur name) I admit my oven tek for innoc is flawed. I was not trying to convert fellow mycoligist to it. I was simply showing a hella simple method of producing an LC within a water bottle. I apologize for any post I come across as dickish. Im just slightly defensive because I know this works. I understand ppl are skiddish to somethin.. I dont facebook or do any other social media. I like his forum for the unique things we talk about. So I encourage you to try it. Hell tell me a better way to do it! But don't say it doesn't work becuz it does.


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I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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OfflineHellyen
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Re: Water bottle LC [Re: Brain Fart]
    #16778562 - 09/02/12 08:40 PM (12 years, 4 months ago)

To answer your ? Khii.

It was a relatively fresh thing of honey. But i would not PC a plastic bottle. No you introduce it in plastic bottle like I said. You should be able to spot contams in your bottle. Fortunately ive always had healthy white myc.  i copied ratio of honey from another tek. I think I use too little why bottle is clear. But it still works so dont overdue it.


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I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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OfflineSimpleFarmer
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Re: Water bottle LC [Re: Hellyen]
    #16778576 - 09/02/12 08:43 PM (12 years, 4 months ago)

You can't always spot contams in lc :wtfsonic: that's why most people use agar , if they are serious ..

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OfflineHellyen
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Re: Water bottle LC [Re: Brain Fart]
    #16778628 - 09/02/12 08:57 PM (12 years, 4 months ago)

Im serius Simple farmer. I always inject test jars before I commit to a grow. PF posted his stuff in 92 or whatever and newbs are directed to those posts. So take it and expand. Easy ass LC. Never done agar but then again I just grow enough for me and the wife.. Plus friends. So I dont go overboard.  can't we all just trip together...?


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I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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InvisibleRahz
Alive Again
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Re: Water bottle LC [Re: Hellyen]
    #16778771 - 09/02/12 09:25 PM (12 years, 4 months ago)

The tek doesn't say how much honey to use.

Quote:

Your bottle cap acts as a sort of filter.




In your favor the initial condition of the bottle water and honey should be pretty close to sterile, so it's not surprising that it works but the cap isn't going to filter the air.


--------------------
rahz

comfort pleasure power love     truth awareness peace I am      I feel      I do     I love  I speak    I see    I know

"If you knew how quickly people forget the dead... you would stop living to impress people." — Christopher Walken

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InvisibleKhii Khwaay
black tooth grin

Registered: 04/16/12
Posts: 2,277
Re: Water bottle LC [Re: Hellyen]
    #16778839 - 09/02/12 09:38 PM (12 years, 4 months ago)

No, what I'm saying is that honey is NOT STERILE, even in an unopened jar. Think about it... that bee is flying around, landing on flowers and kicking up all kinds of dust, spores etc with the pollen it collects. And then coming back to the hive and mixing it all together.

You really have to sterilize your media.

You should also edit your first post to say "TRY AT YOUR OWN RISK, if only for the noobs.

For gods sake man, think about the poor noobs.

:mygoditsfullofstars:

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OfflineHellyen
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Re: Water bottle LC [Re: Brain Fart]
    #16778848 - 09/02/12 09:40 PM (12 years, 4 months ago)

Do the tech as described and you will see how it does. You squeeze all air out. And if bottle cap is just slightly unscrewed you will see how minimal air is introduced in this LC. Just enough clean air. For extra precaution only vent air in your Clean Working Space. Dont use the oven or youll go to hell and argue on shroomery all day. Hell half the good info on this place is a decade old. Why ppl always bitch at new ppl for not using Search. Just my idea. So flame me.. And if you are bold. Try it.


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I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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OfflinePussyFart
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Re: Water bottle LC [Re: Hellyen]
    #16778899 - 09/02/12 09:51 PM (12 years, 4 months ago)

if u find old and outdated info on the main page please post it here:

http://www.shroomery.org/forums/showflat.php/Number/15329243

Quote:

Hellyen said:
Dont use the oven or youll go to hell and argue on shroomery all day.




:kaneclap:

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InvisibleKhii Khwaay
black tooth grin

Registered: 04/16/12
Posts: 2,277
Re: Water bottle LC [Re: Hellyen]
    #16778917 - 09/02/12 09:57 PM (12 years, 4 months ago)

With all due respect, I bought a $35 PC and made a $5 glove box, so, no, I don't think I'll be doing any open-air inoculations of un-sterile media, thank you very much.

I've grown my share of jars full of bacteria LC, and they're actually kind of scary. I'm sure some of that shit can make you sick.

But hey, if it works for you, then keep on truckin', bro.

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InvisibleKhii Khwaay
black tooth grin

Registered: 04/16/12
Posts: 2,277
Re: Water bottle LC [Re: PussyFart]
    #16778940 - 09/02/12 10:02 PM (12 years, 4 months ago)

The problem is there is old outdated info littered throughout the site, in the form of old posts.

When people UTFSE or look at the "related threads" at the bottom of the thread, they can dig up stuff that will basically get them laughed at when they ask about it.

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InvisibleRahz
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Re: Water bottle LC [Re: Hellyen]
    #16778960 - 09/02/12 10:07 PM (12 years, 4 months ago)

Quote:

And if bottle cap is just slightly unscrewed you will see how minimal air is introduced in this LC.




The amount of air isn't as important as what's in the air and how it is filtered. What do you think the micron rating on a loose cap is? If it works it works, but this can't be considered a sterile tek. I remember seeing a post from 6 years ago, a guy used a jar with a 1/16" hole in the lid covered with a piece of foil. It was working for him and others, but there was nothing to keep germs from just floating in at some point. These days there are a variety of ways to build LC containers with a filter and inoculation port.


--------------------
rahz

comfort pleasure power love     truth awareness peace I am      I feel      I do     I love  I speak    I see    I know

"If you knew how quickly people forget the dead... you would stop living to impress people." — Christopher Walken

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OfflineHellyen
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Re: Water bottle LC [Re: Brain Fart]
    #16778991 - 09/02/12 10:14 PM (12 years, 4 months ago)

As i said before. Half the relevant shit from this site is ten years old.  now its a bunch of pokemon generation faggots. Hah. So as I've said do what you want. I'll keep taking this shit to the next lvl.


--------------------
I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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OfflinePussyFart
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Re: Water bottle LC [Re: Hellyen]
    #16779000 - 09/02/12 10:16 PM (12 years, 4 months ago)

Quote:

Hellyen said:
Half the relevant shit from this site is ten years old.  now its a bunch of pokemon generation faggots.




Pokemon was soo ten years ago....lol.....nice try tho

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OfflineHellyen
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Re: Water bottle LC [Re: Brain Fart]
    #16779031 - 09/02/12 10:22 PM (12 years, 4 months ago)

Lol. I said pokemon fags are ten yrs old just like that info. You proved my point. Getting alot of hits ppl must be intrigued by this bullshit. For every hater 1 person try it and lets see results.


--------------------
I'm a mushroom cloud layin' Mother Fucker... Mother Fucker.

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